公开(公告)号：US07618799B2

公开(公告)日：2009-11-17

申请号：US12022789

申请日：2008-01-30

Applicant: Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

Inventor： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

IPC: C12N9/00 ,  C12N15/00 ,  C12N1/04

CPC classification number: C12N15/625 ,  C07K2319/02 ,  C07K2319/036

Abstract: Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

Abstract translation: 提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。 提供了包含细菌分泌信号肽的编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。 本发明的组合物可用于增加正确加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示氨基酸序列的核苷酸序列的分离的核酸分子。 ，以及SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。

公开(公告)号：US07264960B2

公开(公告)日：2007-09-04

申请号：US10502692

申请日：2003-03-21

Applicant: Sol M. Resnick ,  Felipe A. Donate ,  Timothy C. Frank ,  Thomas C. Thyne ,  Paul Foley

Inventor： Sol M. Resnick ,  Felipe A. Donate ,  Timothy C. Frank ,  Thomas C. Thyne ,  Paul Foley

IPC: C12P41/00

CPC classification number: C12P7/18 ,  C12P41/004

Abstract: Glycol ether acetates, and in particular propylene glycol alkyl (or aryl) ether acetates, can be resolved enzymatically by enantioselective hydrolysis with a hydrolase at high concentrations of substrates; in some embodiments, the hydrolase is a lipase. Glycol ethers, and in particular propylene glycol alkyl (or aryl) ethers, can be resolved enzymatically by enantioselective transesterification with a hydrolase, in the presence of an acyl donor, at high concentrations of substrates; in some embodiments, the hydrolase is a lipase.

Abstract translation: 乙二醇醚乙酸酯，特别是丙二醇烷基（或芳基）醚乙酸酯可以通过在高浓度底物下用水解酶进行对映选择性水解来酶解; 在一些实施方案中，水解酶是脂肪酶。 乙二醇醚，特别是丙二醇烷基（或芳基）醚可以通过在酰基供体存在下，在高浓度底物下用水解酶进行对映选择性酯交换酶解解; 在一些实施方案中，水解酶是脂肪酶。

公开(公告)号：US20080193974A1

公开(公告)日：2008-08-14

申请号：US12022789

申请日：2008-01-30

Applicant: Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

Inventor： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

IPC: C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N1/20 ,  C07K14/00 ,  C12N9/90

CPC classification number: C12N15/625 ,  C07K2319/02 ,  C07K2319/036

Abstract: Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

Abstract translation: 提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。 提供了包含细菌分泌信号肽的编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。 本发明的组合物可用于增加正确加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示氨基酸序列的核苷酸序列的分离的核酸分子。 ，以及SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。

公开(公告)号：US06642031B2

公开(公告)日：2003-11-04

申请号：US09570779

申请日：2000-05-14

Applicant: Walter Weyler ,  Sol M. Resnick

Inventor： Walter Weyler ,  Sol M. Resnick

IPC: C12N120

CPC classification number: C12N9/0069 ,  C12N9/86 ,  C12N15/52 ,  C12P7/22 ,  C12P17/10 ,  C12P17/165

Abstract: There is provided novel indole oxidase activity isolated from P. putida, such indole oxidase activity is believed to be useful in the biosynthetic production of indigo from its precursor indole. Also provided are compositions of matter comprising the indole oxidase and methods for producing such.

Abstract translation: 提供从恶臭假单胞菌中分离的新的吲哚氧化酶活性，据信这种吲哚氧化酶活性可用于从其前体吲哚生物合成靛蓝。 还提供了包含吲哚氧化酶的物质组合物及其制备方法。

公开(公告)号：US20160369246A1

公开(公告)日：2016-12-22

申请号：US14898948

申请日：2014-06-18

Applicant: Sungwon LEE ,  Drew D. REGITSKY ,  Sol M. RESNICK ,  CALYSTA, INC.

Inventor： Renee M. Saville ,  Sungwon Lee ,  Drew D. Regitsky ,  Sol M. Resnick ,  Joshua A. Silverman

IPC: C12N9/04 ,  C07C59/08 ,  C07B59/00 ,  C12P7/56 ,  C12N15/74

CPC classification number: C12N9/0006 ,  C07B59/001 ,  C07B2200/05 ,  C07C59/08 ,  C12N1/20 ,  C12N15/74 ,  C12P7/56 ,  C12Y101/01027 ,  C12Y101/01028

Abstract: The present disclosure provides non-naturally occurring c1 microorganisms useful for the production of lactate and related compositions, as well as methods for the biologically production of lactate. In specific embodiments, the present disclosure provides non-naturally occurring methanotrophic bacteria which are useful for producing lactate from c1 substrates.

Abstract translation: 本公开提供了可用于生产乳酸盐和相关组合物的非天然存在的c1微生物以及用于生物地生产乳酸盐的方法。 在具体实施方案中，本公开提供可用于从c1底物产生乳酸盐的非天然存在的甲烷营养细菌。