公开(公告)号：US5243038A

公开(公告)日：1993-09-07

申请号：US114618

申请日：1987-10-29

Applicant: Franco A. Ferrari ,  Charles Richardson ,  James Chambers ,  Stuart C. Causey ,  Thomas J. Pollock ,  Joseph Capello ,  John W. Crissman

Inventor： Franco A. Ferrari ,  Charles Richardson ,  James Chambers ,  Stuart C. Causey ,  Thomas J. Pollock ,  Joseph Capello ,  John W. Crissman

IPC: C07K14/435 ,  C07K14/78 ,  C12N1/06 ,  C12N15/11 ,  C12N15/62 ,  C12N15/70 ,  C12N15/75

CPC classification number: C07K14/78 ,  C07K14/43586 ,  C12N1/06 ,  C12N15/11 ,  C12N15/62 ,  C12N15/70 ,  C12N15/75 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/61

Abstract: Methods are provided for the production of large polypeptides containing repeating sequences of amino acids utilizing biochemical techniques, specifically DNA sequences coding for the expression of the large polypeptides. Systems utilizing exogenous transcriptional and translational regions to control the production of the large polypeptides are also provided.

Abstract translation: 提供了使用生化技术生产含有氨基酸重复序列的大多肽的方法，特别是编码大多肽表达的DNA序列。 还提供了利用外源转录和翻译区以控制大多肽的产生的系统。

公开(公告)号：US06916646B1

公开(公告)日：2005-07-12

申请号：US08876132

申请日：1997-06-23

Applicant: Timothy Fowler ,  Stuart C. Causey

Inventor： Timothy Fowler ,  Stuart C. Causey

IPC: C07K14/24 ,  C12N15/31 ,  C12N15/74 ,  C12P7/60 ,  C12N1/36 ,  C12N1/20

CPC classification number: C07K14/24 ,  C12N15/74 ,  C12P7/60

Abstract: Methods are provided for preparing improved fermentation strains of the family Enterobacteriaceae which comprise the steps of eliminating the cryptic plasmid from the progenitor strain thereby creating the improved strain. Methods for reducing the mobilization properties of resident plasmids in an Enterobacteriaceae strain containing a cryptic plasmid are also provided. The present invention provides the nucleic acid sequence of pS, a cryptic plasmid found in Pantoea which can be used to identify the cryptic plasmid in strains of Enterobacteriaceae.

Abstract translation: 提供了用于制备肠杆菌科的改良发酵菌株的方法，其包括从祖细胞菌株中消除神经质粒的步骤，从而产生改良的菌株。 还提供了用于降低含有隐性质粒的肠杆菌科菌株中驻留质粒的动员特性的方法。 本发明提供了在Pantoea中发现的可用于鉴定肠杆菌科菌株中的隐性质粒的隐神质的pS的核酸序列。