公开(公告)号：US08481050B2

公开(公告)日：2013-07-09

申请号：US12310309

申请日：2007-08-22

申请人： Qui-Lim Choo ,  Jang Han ,  Michael Houghton ,  Taewoo Kwon ,  Hyun Chul Song ,  Yifei Zhu

发明人： Qui-Lim Choo ,  Jang Han ,  Michael Houghton ,  Taewoo Kwon ,  Hyun Chul Song ,  Yifei Zhu

IPC分类号： A61K48/00 ,  A61K50/00

CPC分类号： C12N7/00 ,  A61K2039/525 ,  C07K14/005 ,  C07K2319/61 ,  C12N2760/16022 ,  C12N2770/24243 ,  G01N2333/186 ,  G01N2500/10

摘要： A tissue culture system for production of infectious hepatitis C virus is described. In particular, the invention provides recombinant monocistronic and bicistronic genomic constructs for production of virus, including constructs for production of wild-type HCV type 2a strain JFH1 and constructs for production of chimeric viruses comprising HCV proteins from strain JFH1 and a second HCV isolate. Constructs of the invention also include a reporter gene to facilitate measurement of RNA replication and viral infectivity in cultures. The cell culture system may also include various factors that improve viral replication or infectivity. In addition, a neutralization assay using HCV grown in cell culture is described.

摘要翻译： 描述了用于生产感染性丙型肝炎病毒的组织培养系统。 特别地，本发明提供了用于产生病毒的重组单顺反子和双顺反子基因组构建体，包括用于产生野生型HCV 2a型菌株JFH1的构建体和用于产生包含来自菌株JFH1的HCV蛋白质和第二HCV分离物的嵌合病毒的构建体。 本发明的构建体还包括有利于测量培养物中RNA复制和病毒感染性的报告基因。 细胞培养系统还可以包括改善病毒复制或感染性的各种因素。 此外，描述了使用在细胞培养物中生长的HCV的中和测定。

公开(公告)号：US20110070658A1

公开(公告)日：2011-03-24

申请号：US12784280

申请日：2010-05-20

申请人： William J. Rutter ,  Jang H. Han ,  Taewoo Kwon

发明人： William J. Rutter ,  Jang H. Han ,  Taewoo Kwon

IPC分类号： G01N33/566 ,  C07K19/00 ,  G01N30/00

CPC分类号： G01N33/723 ,  G01N33/526 ,  G01N33/558 ,  G01N33/721 ,  Y10T436/25375

摘要： Described herein are systems and methods for assaying a sample to quantitatively determine the percentage of glycated hemoglobin in the sample.

摘要翻译： 本文描述了用于测定样品以定量测定样品中糖化血红蛋白百分比的系统和方法。

公开(公告)号：US20100227311A1

公开(公告)日：2010-09-09

申请号：US12310309

申请日：2007-08-22

申请人： Jang Han ,  Qui-Lim Choo ,  Michael Houghton ,  Taewoo Kwon ,  Hyun Chul Song ,  Yifei Zhu

发明人： Jang Han ,  Qui-Lim Choo ,  Michael Houghton ,  Taewoo Kwon ,  Hyun Chul Song ,  Yifei Zhu

IPC分类号： C12Q1/70 ,  C12N15/74 ,  C12N5/10 ,  C12N7/00 ,  G01N33/567

CPC分类号： C12N7/00 ,  A61K2039/525 ,  C07K14/005 ,  C07K2319/61 ,  C12N2760/16022 ,  C12N2770/24243 ,  G01N2333/186 ,  G01N2500/10

摘要： A tissue culture system for production of infectious hepatitis C virus is described. In particular, the invention provides recombinant monocistronic and bicistronic genomic constructs for production of virus, including constructs for production of wild-type HCV type 2a strain JFH1 and constructs for production of chimeric viruses comprising HCV proteins from strain JFH1 and a second HCV isolate. Constructs of the invention also include a reporter gene to facilitate measurement of RNA replication and viral infectivity in cultures. The cell culture system may also include various factors that improve viral replication or infectivity. In addition, a neutralization assay using HCV grown in cell culture is described.

摘要翻译： 描述了用于生产感染性丙型肝炎病毒的组织培养系统。 特别地，本发明提供了用于产生病毒的重组单顺反子和双顺反子基因组构建体，包括用于产生野生型HCV 2a型菌株JFH1的构建体和用于产生包含来自菌株JFH1的HCV蛋白质和第二HCV分离物的嵌合病毒的构建体。 本发明的构建体还包括有利于测量培养物中RNA复制和病毒感染性的报告基因。 细胞培养系统还可以包括改善病毒复制或感染性的各种因素。 此外，描述了使用在细胞培养物中生长的HCV的中和测定。

公开(公告)号：US08703494B2

公开(公告)日：2014-04-22

申请号：US12784280

申请日：2010-05-20

申请人： William J. Rutter ,  Jang H. Han ,  Taewoo Kwon

发明人： William J. Rutter ,  Jang H. Han ,  Taewoo Kwon

IPC分类号： G01N33/72 ,  C12M1/34 ,  G01N33/52

CPC分类号： G01N33/723 ,  G01N33/526 ,  G01N33/558 ,  G01N33/721 ,  Y10T436/25375

摘要： Described herein are systems and methods for assaying a sample to quantitatively determine the percentage of glycated hemoglobin in the sample.

摘要翻译： 本文描述了用于测定样品以定量测定样品中糖化血红蛋白百分比的系统和方法。

公开(公告)号：US09459262B2

公开(公告)日：2016-10-04

申请号：US14196384

申请日：2014-03-04

申请人： William J. Rutter ,  Jang H. Han ,  Taewoo Kwon

发明人： William J. Rutter ,  Jang H. Han ,  Taewoo Kwon

IPC分类号： G01N33/558 ,  G01N33/72 ,  G01N33/52

CPC分类号： G01N33/723 ,  G01N33/526 ,  G01N33/558 ,  G01N33/721 ,  Y10T436/25375

摘要： Described herein are systems and methods for the quantitative analysis of a sample comprising glycated hemoglobin. Also described herein are systems and methods for assaying a sample to quantitatively determine the percentage of glycated hemoglobin in the sample, for example, in a blood sample. The methods and systems described herein may be used in a point-of-care device as well as in other in vitro diagnostic devices.

摘要翻译： 本文描述了用于定量分析包含糖化血红蛋白的样品的系统和方法。 本文还描述了用于测定样品以定量测定样品中糖化血红蛋白的百分比的系统和方法，例如在血液样品中。 本文描述的方法和系统可以用在护理装置以及其它体外诊断装置中。

公开(公告)号：US20110073220A1

公开(公告)日：2011-03-31

申请号：US12955862

申请日：2010-11-29

申请人： Taewoo Kwon ,  Chisang Yoon

发明人： Taewoo Kwon ,  Chisang Yoon

IPC分类号： B22D11/12 ,  B22D27/02 ,  C21D8/02

CPC分类号： C21D1/673 ,  C21D9/48 ,  C21D11/00 ,  C21D2211/008 ,  F27B9/20

摘要： The present invention relates to a method of continuous hardening press and an apparatus thereof. The process of continuous press hardening, includes: slab casting that makes a slab by continuous-casting molten steel refined by an electric furnace or a blast furnace, and then reheats and hot-rolls the slab into a hot-rolled steel sheet; blanking that conveys the hot-rolled steel sheet to a blank through a sealed oven and blanks the hot-rolled steel sheet, after the slab casting; and press hardening that conveys the hot-rolled steel sheet to a press die and presses the hot-rolled steel sheet, after the blanking. According to the embodiments of the present invention, since a high-temperature steel sheet formed by hot rolling is directly put into press hardening, reheating is not required such that the entire process is simplified and reduction of manufacturing cost can be expected.

摘要翻译： 本发明涉及一种连续硬化冲压机的方法及其装置。 连续加压硬化的过程包括：通过由电炉或高炉精炼的连续铸造钢水制成板坯，然后将板坯再加热和热轧成热轧钢板; 通过密封炉将热轧钢板输送到坯料上，在板坯铸造之后对热轧钢板进行冲裁; 以及将热轧钢板输送到压模上的冲压硬化，在冲裁后压制热轧钢板。 根据本发明的实施例，由于通过热轧形成的高温钢板直接进行加压硬化，因此不需要再加热，从而简化整个过程，并且可以预期制造成本的降低。