公开(公告)号：US20230390074A1

公开(公告)日：2023-12-07

申请号：US18206451

申请日：2023-06-06

申请人： Encore Medical, LP DBA DJO Surgical

发明人： Joseph P. Iannotti

IPC分类号： A61F2/40 ,  C12N15/86 ,  A61F2/46

CPC分类号： A61F2/4014 ,  C12N15/86 ,  A61F2/4003 ,  A61F2/4059 ,  A61F2/4612 ,  C12N2770/24243 ,  A61F2002/30253

摘要： A humeral prosthetic head has a non-spherical articulation surface coupled with an intermediate component connecting the head and the humerus, the intermediate component connected to the epiphysis, metaphysis, or diaphysis, or to one or more additional components connected to the humerus. The intermediate portion provides for axial and angular offset of the head with respect to a connection to the humerus, using a curvilinear tapered engagement.

公开(公告)号：US09683269B2

公开(公告)日：2017-06-20

申请号：US14514023

申请日：2014-10-14

申请人： Hvidovre Hospital ,  The United States of America, as Represented by the Secretary, Department of Health and Human Services

发明人： Judith M. Gottwein ,  Troels Kasper Hoyer Scheel ,  Robert Purcell ,  Jens Bukh

IPC分类号： C12N7/00 ,  C12Q1/70 ,  C12N15/86 ,  G01N33/50

CPC分类号： C12Q1/18 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/24221 ,  C12N2770/24243 ,  C12Q1/707 ,  G01N33/5008

摘要： The present invention relates to molecular approaches to the production of nucleic acid sequences, which comprises the genome of infectious hepatitis C virus. In particular, the invention provides nucleic acid sequences which comprise the genomes of infectious hepatitis C viruses of either genotype 3a (strain S52) or genotype 4a (strain ED43). The invention therefore relates to the use of the nucleic acid sequences and polypeptides encoded by all or part of the sequences in the development of vaccines and diagnostic assays for HCV and in the development of screening assays for the identification of antiviral agents for HCV. The invention therefore also relates to the use of viral particles derived from laboratory animals infected with S52 and ED43 viruses.

公开(公告)号：US09234184B2

公开(公告)日：2016-01-12

申请号：US14009049

申请日：2012-03-30

申请人： Takaji Wakita ,  Tomoko Date ,  Yasuhito Tanaka ,  Masashi Mizokami

发明人： Takaji Wakita ,  Tomoko Date ,  Yasuhito Tanaka ,  Masashi Mizokami

IPC分类号： C12N7/08 ,  C12N7/00 ,  C12N7/04 ,  C12N15/86 ,  C07K16/10 ,  C12Q1/70 ,  C07K16/08 ,  C07K14/005 ,  A61K39/12 ,  A61K39/00

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  C07K14/005 ,  C07K16/082 ,  C07K16/109 ,  C12N15/86 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24231 ,  C12N2770/24234 ,  C12N2770/24243 ,  C12N2770/24251 ,  C12Q1/707 ,  C12Q2600/136 ,  C12Q2600/156

摘要： A subgenomic replicon RNA (nucleic acid) having an excellent autonomously replicating ability and a fullgenomic replicon RNA (nucleic acid) having an excellent autonomously replicating ability and infectious HCV particle-producing ability, each derived from a novel HCV of genotype 1b, are provided. Particularly, a subgenomic replicon RNA (nucleic acid) and a fullgenomic replicon RNA (nucleic acid), each derived from an HCV genome of the NC1 strain which is a novel HCV genotype 1b isolated from a patient with acute severe hepatitis C, are provided.

摘要翻译： 提供了具有优异的自主复制能力的亚基因组复制子RNA（核酸）和具有优异的自主复制能力和感染性HCV颗粒产生能力的全基因组复制子RNA（核酸），各自源自基因型1b的新型HCV。 特别地，提供了从NC1菌株的HCV基因组衍生的亚基因组复制子RNA（核酸）和全基因组复制子RNA（核酸），其是从急性重症丙型肝炎患者分离的新型HCV基因型1b。

公开(公告)号：US08481050B2

公开(公告)日：2013-07-09

申请号：US12310309

申请日：2007-08-22

申请人： Qui-Lim Choo ,  Jang Han ,  Michael Houghton ,  Taewoo Kwon ,  Hyun Chul Song ,  Yifei Zhu

发明人： Qui-Lim Choo ,  Jang Han ,  Michael Houghton ,  Taewoo Kwon ,  Hyun Chul Song ,  Yifei Zhu

IPC分类号： A61K48/00 ,  A61K50/00

CPC分类号： C12N7/00 ,  A61K2039/525 ,  C07K14/005 ,  C07K2319/61 ,  C12N2760/16022 ,  C12N2770/24243 ,  G01N2333/186 ,  G01N2500/10

摘要： A tissue culture system for production of infectious hepatitis C virus is described. In particular, the invention provides recombinant monocistronic and bicistronic genomic constructs for production of virus, including constructs for production of wild-type HCV type 2a strain JFH1 and constructs for production of chimeric viruses comprising HCV proteins from strain JFH1 and a second HCV isolate. Constructs of the invention also include a reporter gene to facilitate measurement of RNA replication and viral infectivity in cultures. The cell culture system may also include various factors that improve viral replication or infectivity. In addition, a neutralization assay using HCV grown in cell culture is described.

摘要翻译： 描述了用于生产感染性丙型肝炎病毒的组织培养系统。 特别地，本发明提供了用于产生病毒的重组单顺反子和双顺反子基因组构建体，包括用于产生野生型HCV 2a型菌株JFH1的构建体和用于产生包含来自菌株JFH1的HCV蛋白质和第二HCV分离物的嵌合病毒的构建体。 本发明的构建体还包括有利于测量培养物中RNA复制和病毒感染性的报告基因。 细胞培养系统还可以包括改善病毒复制或感染性的各种因素。 此外，描述了使用在细胞培养物中生长的HCV的中和测定。

公开(公告)号：US20130115592A1

公开(公告)日：2013-05-09

申请号：US13734630

申请日：2013-01-04

申请人： Tokyo Metropolitan Institute Of Medical Science ,  Toray Industries, Inc.

发明人： Takaji WAKITA ,  Takanobu KATO ,  Tomoko DATE ,  Michiko MIYAMOTO ,  Ralf BARTENSCHLAGER ,  Jun-ichi TANABE ,  Saburo SONE

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K2039/525 ,  C12N15/86 ,  C12N2770/24221 ,  C12N2770/24243 ,  C12N2840/203 ,  C12Q1/6883

摘要： The present invention provides modified hepatitis C virus genomic RNA, comprising nucleotide sequences of genomic RNA portions of two or more types of hepatitis C viruses, which comprises a 5′ untranslated region, a core protein coding sequence, an E1 protein coding sequence, a p7 protein coding sequence, an E2 protein coding sequence, an NS2 protein coding sequence, an NS3 protein coding sequence, an NS4A protein coding sequence, an NS4B protein coding sequence, an NS5A protein coding sequence, an NS5B protein coding sequence, and a 3′ untranslated region, and which can be autonomously replicated. In particular, the present invention relates to modified hepatitis C virus genomic RNA, which can be autonomously replicated by substitution of the RNA sequence portion encoding NS3, NS4, NS5A, and NS5B proteins of hepatitis C virus genomic RNA with a partial RNA sequence encoding NS3, NS4, NS5A, and NS5B proteins of a JFH1 strain shown in SEQ ID NO: 1.

摘要翻译： 本发明提供了修饰的丙型肝炎病毒基因组RNA，其包含两种或更多种类型的丙型肝炎病毒的基因组RNA部分的核苷酸序列，其包含5'非翻译区，核心蛋白编码序列，E1蛋白编码序列，p7 蛋白质编码序列，E2蛋白编码序列，NS2蛋白编码序列，NS3蛋白编码序列，NS4A蛋白编码序列，NS4B蛋白编码序列，NS5A蛋白编码序列，NS5B蛋白编码序列和3' 非翻译区域，可以自主复制。 特别地，本发明涉及经修饰的丙型肝炎病毒基因组RNA，其可以通过用编码NS3的部分RNA序列替代编码丙型肝炎病毒基因组RNA的NS3，NS4，NS5A和NS5B蛋白的RNA序列部分来自主复制 ，NS4，NS5A和NS5B蛋白，如SEQ ID NO：1所示。

公开(公告)号：US08367401B2

公开(公告)日：2013-02-05

申请号：US11975658

申请日：2007-10-19

申请人： Stanley M. Lemon ,  MinKyung Yi

发明人： Stanley M. Lemon ,  MinKyung Yi

IPC分类号： C12Q1/70 ,  C12N15/63

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N7/00 ,  C12N2770/24243 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203

摘要： The present invention provides a replication competent hepatitis C virus that includes a heterologous polynucleotide. The invention also includes methods for modifying a hepatitis C virus polynucleotide, selecting a replication competent hepatitis C virus polynucleotide, detecting a replication competent hepatitis C virus polynucleotide, and identifying a compound that inhibits replication of a hepatitis C virus polynucleotide.

摘要翻译： 本发明提供了包含异源多核苷酸的复制型丙型肝炎病毒。 本发明还包括用于修饰丙型肝炎病毒多核苷酸，选择复制感染型丙型肝炎病毒多核苷酸，检测可复制感染性丙型肝炎病毒多核苷酸以及鉴定抑制丙型肝炎病毒多核苷酸复制的化合物的方法。

公开(公告)号：US20110289610A1

公开(公告)日：2011-11-24

申请号：US13054356

申请日：2009-07-15

申请人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

发明人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

IPC分类号： A01K67/027 ,  C07K14/18 ,  C12N15/63 ,  C12N5/10 ,  C12N15/51 ,  C12N7/01

CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24243

摘要： An HCV/GBV-B chimeric virus which maintains the replication function of HCV and is capable of infecting tamarin is disclosed in order to construct an HCV animal model which can be used as a development or evaluation system for therapeutic agents for HCV. The HCV/GBV-B chimeric RNA comprises an RNA of hepatitis C virus and an RNA of GB virus-B, wherein the RNA of hepatitis C virus comprises an RNA encoding leucine at the 1804th position and lysine at the 1966th position in the amino acid sequence of the polyprotein of hepatitis C virus.

摘要翻译： 公开了保持HCV的复制功能并能够感染t am素的HCV / GBV-B嵌合病毒，以构建可用作HCV治疗剂的开发或评估系统的HCV动物模型。 HCV / GBV-B嵌合RNA包含丙型肝炎病毒的RNA和GB病毒B的RNA，其中丙型肝炎病毒的RNA包含编码第1804位亮氨酸的RNA，氨基酸中第1966位的赖氨酸 丙型肝炎病毒多蛋白序列。

公开(公告)号：US20100062419A1

公开(公告)日：2010-03-11

申请号：US12581597

申请日：2009-10-19

申请人： Charles Rice ,  Matthew J. Evans ,  Brett D. Lindenbach ,  Christopher Jones

发明人： Charles Rice ,  Matthew J. Evans ,  Brett D. Lindenbach ,  Christopher Jones

IPC分类号： C12Q1/70 ,  C12N7/00 ,  C07H21/04

CPC分类号： C12N7/00 ,  A61K2039/525 ,  C07K2319/61 ,  C12N15/86 ,  C12N2770/24243 ,  C12N2770/24251 ,  C12N2840/203 ,  C12Q1/18 ,  G01N33/5767 ,  G01N2333/18 ,  G01N2500/00

摘要： The present invention provides infectious recombinant Hepatitis C Viruses (HCV), and vectors, cells and animals comprising the same. The present invention provides methods of producing infectious recombinant HCV, and their use in identifying anti-HCV therapeutic agents, as well as sequences of HCV associated with HCV pathogenesis.

摘要翻译： 本发明提供感染性重组丙型肝炎病毒（HCV），以及包含其的载体，细胞和动物。 本发明提供生产感染性重组HCV的方法及其在鉴定抗HCV治疗剂中的用途以及与HCV发病机制相关的HCV序列。

公开(公告)号：US20090042181A1

公开(公告)日：2009-02-12

申请号：US11898468

申请日：2007-09-12

申请人： Takaji Wakita ,  Takanobu Kato ,  Tomoko Date ,  Michiko Miyamoto

发明人： Takaji Wakita ,  Takanobu Kato ,  Tomoko Date ,  Michiko Miyamoto

IPC分类号： C12Q1/70 ,  C07H21/04 ,  C07H21/02 ,  C12P19/34 ,  C12N15/87 ,  C07K2/00 ,  C12N5/10

CPC分类号： C07K14/005 ,  C12N15/86 ,  C12N2770/24222 ,  C12N2770/24243

摘要： The present invention relates to a gene derived from a novel fulminant hepatitis C virus strain, an HCV replicon RNA with a high replication efficiency obtained using the gene, and an HCV replicon-replicating cell transfected with the replicon RNA. When the HCV replicon RNA and the HCV replicon-replicating cell of the present invention are used, HCV proteins can be continuously produced in a large amount.

摘要翻译： 本发明涉及来自新型暴发性丙型肝炎病毒株的基因，使用该基因获得的具有高复制效率的HCV复制子RNA和用复制子RNA转染的HCV复制子复制细胞。 当使用本发明的HCV复制子RNA和HCV复制子复制细胞时，可以大量连续生产HCV蛋白质。

公开(公告)号：US20060246424A1

公开(公告)日：2006-11-02

申请号：US11390632

申请日：2006-03-28

申请人： George Kukolj ,  Arnim Pause

发明人： George Kukolj ,  Arnim Pause

IPC分类号： C12Q1/70 ,  C12N5/08

CPC分类号： C07K14/005 ,  A61K2039/525 ,  C12N15/86 ,  C12N2770/24222 ,  C12N2770/24243 ,  C12N2840/206 ,  C12Q1/6897

摘要： A unique HCV RNA molecule is provided having an enhanced efficiency of establishing cell culture replication. Novel adaptive mutations have been identified within the HCV non-structural region that improves the efficiency of establishing persistently replicating HCV RNA in cell culture. This self-replicating polynucleotide molecule contains, contrary to all previous reports, a 5′-NTR that can be either an A as an alternative to the G already disclosed and therefore provides an alternative to existing systems comprising a self-replicating HCV RNA molecule. The G→A mutation gives rise to HCV RNA molecules that, in conjunction with mutations in the HCV non-structural region, such as the G(2042)C/R mutations, possess greater efficiency of transduction and/or replication. These RNA molecules when transfected in a cell line are useful for evaluating potential inhibitors of HCV replication.