公开(公告)号：US06673576B1

公开(公告)日：2004-01-06

申请号：US08913816

申请日：1998-05-13

Applicant: Yoshihiro Usuda ,  Hisashi Kawasaki ,  Megumi Shimaoka ,  Takashi Utagawa

Inventor： Yoshihiro Usuda ,  Hisashi Kawasaki ,  Megumi Shimaoka ,  Takashi Utagawa

IPC: C12D1930

CPC classification number: C12N9/12 ,  C12P19/32

Abstract: The present invention relates to a process for producing 5′-inosinic acid or 5′-guanylic acid for use in seasonings or the like from inosine or guanosine or a precursor thereof using an adenosine triphosphate (ATP)-regenerating microorganism containing a DNA encoding a protein that has the activity of forming 5′-inosinic acid or 5′-guanylic acid from inosine or guanosine. Further, the present invention relates to a novel protein having the inosine-guanosine kinase activity, a gene encoding said protein, a recombinant DNA containing said gene, and a microorganism which is transformed with said recombinant DNA.

Abstract translation: 本发明涉及一种使用三磷酸腺苷（ATP）再生微生物从肌苷或鸟苷或其前体制备用于调味料等的5'-肌苷酸或5'-鸟苷酸的方法，该微生物含有编码 具有从肌苷或鸟苷形成5'-肌苷酸或5'-鸟苷酸活性的蛋白质。 此外，本发明涉及具有肌苷 - 鸟苷激酶活性的新型蛋白质，编码所述蛋白质的基因，含有所述基因的重组DNA和用所述重组DNA转化的微生物。

公开(公告)号：US06355472B2

公开(公告)日：2002-03-12

申请号：US09727578

申请日：2000-12-04

Applicant: Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

Inventor： Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

IPC: C12N120

CPC classification number: C12N9/16 ,  C12P19/30

Abstract: A method for producing nucleoside-5′-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phosphate group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5′-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.

Abstract translation: 通过使用对核苷具有增加的亲和力的酸性磷酸酶和/或在pH3.0至pH3.0的pH升高的温度稳定性磷酸化磷酸基团供体的核苷而廉价且高收率地制备核苷-5'-磷酸酯的方法， 5.5，生成核苷-5'-磷酸酯。 还提供了对核苷具有增加的亲和力和/或提高的温度稳定性的突变型酸性磷酸酶。

公开(公告)号：US6010851A

公开(公告)日：2000-01-04

申请号：US750145

申请日：1997-01-21

Applicant: Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

Inventor： Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

IPC: C12N15/09 ,  C12N9/16 ,  C12N15/55 ,  C12P19/30 ,  C12P19/32 ,  C12R1/01 ,  C12R1/19 ,  C12Q1/68

CPC classification number: C12N9/16 ,  C12P19/32

Abstract: Nucleoside-5'-phosphate ester is produced inexpensively and efficiently by allowing an acid phosphatase, especially an acid phosphatase having a lowered phosphomonoesterase activity to act under a condition of pH 3.0 to 5.5 on a nucleoside and a phosphate group donor selected from the group consisting of polyphosphoric acid or a salt thereof, phenylphosphoric acid or a salt thereof, and carbamyl phosphate or a salt thereof to produce nucleoside-5'-phosphate ester, and collecting it.

Abstract translation: PCT No.PCT / JP96 / 01402 Sec。 371日期1997年1月21日 102（e）1997年1月21日PCT PCT 1996年5月24日PCT公布。 WO96 / 37603 PCT出版物 日期：1996年11月28日核苷-5'-磷酸酯是通过使酸性磷酸酶，特别是磷酸单酯酶活性降低的酸性磷酸酶在pH3.0〜5.5的条件下在核苷和磷酸基供体上起作用而廉价而有效地生产的 选自多磷酸或其盐，苯基磷酸或其盐，磷酸氨基戊酯或其盐，以制备核苷-5'-磷酸酯，并将其收集。

公开(公告)号：US4458016A

公开(公告)日：1984-07-03

申请号：US356405

申请日：1982-03-09

Applicant: Shigeru Yamanaka ,  Takashi Utagawa ,  Tadao Kobayashi

Inventor： Shigeru Yamanaka ,  Takashi Utagawa ,  Tadao Kobayashi

IPC: C12P19/28 ,  C07H19/056 ,  C12P19/38 ,  C12R1/01 ,  C12R1/025 ,  C12R1/05 ,  C12R1/06 ,  C12R1/07 ,  C12R1/08 ,  C12R1/13 ,  C12R1/15 ,  C12R1/18 ,  C12R1/185 ,  C12R1/19 ,  C12R1/20 ,  C12R1/22 ,  C12R1/265 ,  C12R1/37 ,  C12R1/38 ,  C12R1/42 ,  C12R1/425 ,  C12R1/44 ,  C12R1/45 ,  C12R1/63 ,  C12R1/64 ,  C12R1/72 ,  C12R1/74 ,  C12R1/85 ,  C12R1/865 ,  C12P17/04 ,  C12P17/10 ,  C12P17/16

CPC classification number: C12P19/38 ,  C07H19/056

Abstract: The production of a 1-.beta.-D-ribofuranosyl-1,2,4-triazole of the formula ##STR1## wherein R is hydroxy, amino, or alkoxy, is carried out by reacting a 1,2,4 triazole of formula ##STR2## wherein R is hydroxy, amino, or alkoxy group, with a greater than equimolar amount of a ribofuranosyl group donor in the presence of a nucleoside phosphorylase preparation at a temperature in the range from 40.degree. to 65.degree. C. for more than 10 minutes, and recovering a 1-.beta.-D-ribofuranosyl-1,2,4-triazole from the reaction solution. The temperature is preferably from 55.degree. to 65.degree. C. and the time is preferably 10 minutes to 20 hours. An elevated temperature higher than the usual enzyme reaction temperature isused to prevent undesirable enzymatic reactions.

Abstract translation: 其中R是羟基，氨基或烷氧基的式“IMAGE”的1-β-D-呋喃核糖基-1,2,4-三唑的制备是通过使式 IMAGE>其中R是羟基，氨基或烷氧基，在核苷磷酸化酶制剂存在下，在40-65℃的温度范围内具有大于等摩尔量的呋喃核糖基供体，超过10 分钟，并从反应溶液中回收1-β-D-呋喃核糖基-1,2,4-三唑。 温度优选为55〜65℃，优选为10分钟〜20小时。 使用高于通常酶反应温度的升高温度来防止不期望的酶反应。

公开(公告)号：US4371613A

公开(公告)日：1983-02-01

申请号：US930046

申请日：1978-08-01

Applicant: Takashi Utagawa ,  Takeshi Miyoshi ,  Hirokazu Morisawa ,  Akihiro Yamazaki ,  Fumihiro Yoshinaga ,  Koji Mitsugi

Inventor： Takashi Utagawa ,  Takeshi Miyoshi ,  Hirokazu Morisawa ,  Akihiro Yamazaki ,  Fumihiro Yoshinaga ,  Koji Mitsugi

IPC: C07H19/16 ,  C12P19/40 ,  C12P19/32

CPC classification number: C12P19/40 ,  C07H19/16

Abstract: Purine-arabinosides and a method for making purine-arabinosides are disclosed. The method comprises contacting an arabinose donor and a purine source in the presence of an effective amount of enzyme produced by a bacterium and capable of transarabinosylation from the arabinose donor to the purine source, whereby a 9-(.beta.-D-arabinofuranosyl)-purine is produced.

Abstract translation: 公开了嘌呤 - 阿拉伯糖苷和制备嘌呤 - 阿拉伯糖苷的方法。 该方法包括在有效量的由细菌产生并能够从阿拉伯糖供体转运到嘌呤源的阿拉伯糖基化反应的存在下使阿拉伯糖供体和嘌呤源接触，由此将9-（β-D-阿拉伯呋喃糖基） - 嘌呤 生产。

公开(公告)号：US06207435B1

公开(公告)日：2001-03-27

申请号：US09417090

申请日：1999-10-13

Applicant: Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

Inventor： Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

IPC: C12N916

CPC classification number: C12N9/16 ,  C12P19/30

Abstract: A method for producing nucleoside-5′-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5′-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.

Abstract translation: 通过使用对核苷具有增加的亲和性的酸性磷酸酶和/或在pH3.0至pH3.0的pH升高的温度稳定性，磷酸化具有磷酸基供体的核苷的方法，以高产率生产核苷-5'-磷酸酯 5.5，生成核苷-5'-磷酸酯。 还提供了对核苷具有增加的亲和力和/或提高的温度稳定性的突变型酸性磷酸酶。

公开(公告)号：US06015697A

公开(公告)日：2000-01-18

申请号：US975698

申请日：1997-11-21

Applicant: Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

Inventor： Yasuhiro Mihara ,  Takashi Utagawa ,  Hideaki Yamada ,  Yasuhisa Asano

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N15/00 ,  C12N15/55 ,  C12P19/30 ,  C12P19/36 ,  C12R1/01 ,  C12R1/185 ,  C12R1/19 ,  C12R1/22 ,  C12R1/425 ,  C12P19/38

CPC classification number: C12N9/16 ,  C12P19/30

Abstract: A method for producing nucleoside-5'-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5'-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.

Abstract translation: 通过使用对核苷具有增加的亲和性的酸性磷酸酶和/或在pH3.0至pH3.0的pH升高的温度稳定性，磷酸化具有磷酸基供体的核苷的方法，以高产率生产核苷-5'-磷酸酯 5.5，生成核苷-5'-磷酸酯。 还提供了对核苷具有增加的亲和力和/或提高的温度稳定性的突变型酸性磷酸酶。