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公开(公告)号：US09017976B2
公开(公告)日：2015-04-28
申请号：US13503999
申请日：2010-11-17
申请人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Christopher Collard , Janice G. Pero , R. Rogers Yocum
发明人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Christopher Collard , Janice G. Pero , R. Rogers Yocum
CPC分类号： A61B6/0442 , B29C70/04 , B29L2031/753 , C12N9/1205 , C12N9/88 , C12N15/52 , C12P7/40 , C12P7/46
摘要： This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.
摘要翻译： 本发明涉及从遗传工程和代谢进化的微生物生产化学物质。 已经建立了化学生产的改进，并确定了导致这些改进的特定突变。 在鉴定在遗传工程化生产琥珀酸的细菌菌株的代谢进化期间发生的突变的具体实例。 本发明还提供了用于评估在增加的琥珀酸生产的代谢进化期间选择的突变的工业适用性的方法。 本发明进一步提供了工程改造成具有在代谢进化期间选择的突变的微生物，并有助于改善琥珀酸，其它有机酸和其它商业利益的化学品的生产。
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2.发明申请METABOLIC EVOLUTION OF ESCHERICHIS COLI STRAINS THAT PRODUCE ORGANIC ACIDS 有权生产有机酸的ESCHERICHIS COLI菌株的代谢演化公开(公告)号：US20120202259A1
公开(公告)日：2012-08-09
申请号：US13394176
申请日：2010-11-17
申请人： Tammy Grabar , Wei Gong , R. Rogers Yocum
发明人： Tammy Grabar , Wei Gong , R. Rogers Yocum
CPC分类号： C07K14/245 , C12P7/40 , C12P7/46 , C12P7/54
摘要： This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.
摘要翻译： 本发明涉及先前优化用于在发酵条件下以商业上显着的量生产有机酸的微生物生物的代谢进化，其中使用己糖作为最小矿物培养基中的唯一碳源。 作为这种代谢进化的结果，微生物生物体获得使用来自纤维素材料的戊糖的生长能力，同时保持原始生长动力学，有机酸生产速率和使用己糖作为碳源的能力 。 本发明还公开了赋予在生产商业上大量有机酸中同时使用己糖和戊糖的能力的微生物中的遗传变化。
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公开(公告)号：US20070037265A1
公开(公告)日：2007-02-15
申请号：US11501137
申请日：2006-08-08
申请人： Shengde Zhou , Lonnie Ingram , Keelnatham Shanmugam , Lorraine Yomano , Tammy Grabar , Jonathan Moore
发明人： Shengde Zhou , Lonnie Ingram , Keelnatham Shanmugam , Lorraine Yomano , Tammy Grabar , Jonathan Moore
摘要： The present invention provides derivatives of ethanologenic Escherichia coli K011 constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.
摘要翻译： 本发明提供了用于生产乳酸的酿酒酵母大肠杆菌K011的衍生物。 本发明的转化的大肠杆菌通过删除编码竞争性途径的基因，随后是具有改进性能的突变体的基于生长的选择来制备。 这些转化的大肠杆菌可用于提供用于食品和工业应用的增加的乳酸供应。
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公开(公告)号：US20120220000A1
公开(公告)日：2012-08-30
申请号：US13503999
申请日：2010-11-17
申请人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Chirstopher Collard , Janice G. Pero , R. Rogers Yocum
发明人： Wei Gong , Sudhanshu Dole , Tammy Grabar , Andrew Chirstopher Collard , Janice G. Pero , R. Rogers Yocum
CPC分类号： A61B6/0442 , B29C70/04 , B29L2031/753 , C12N9/1205 , C12N9/88 , C12N15/52 , C12P7/40 , C12P7/46
摘要： This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.
摘要翻译： 本发明涉及从遗传工程和代谢进化的微生物生产化学物质。 已经建立了化学生产的改进，并确定了导致这些改进的特定突变。 在鉴定在遗传工程化生产琥珀酸的细菌菌株的代谢进化期间发生的突变的具体实例。 本发明还提供了用于评估在增加的琥珀酸生产的代谢进化期间选择的突变的工业适用性的方法。 本发明进一步提供了工程改造成具有在代谢进化期间选择的突变的微生物，并有助于改善琥珀酸，其它有机酸和其它商业利益的化学品的生产。
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5.发明授权公开(公告)号：US08871489B2
公开(公告)日：2014-10-28
申请号：US13394176
申请日：2010-11-17
申请人： Tammy Grabar , Wei Gong , R. Rogers Yocum
发明人： Tammy Grabar , Wei Gong , R. Rogers Yocum
CPC分类号： C07K14/245 , C12P7/40 , C12P7/46 , C12P7/54
摘要： This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.
摘要翻译： 本发明涉及先前优化用于在发酵条件下以商业上显着的量生产有机酸的微生物生物的代谢进化，其中使用己糖作为最小矿物培养基中的唯一碳源。 作为这种代谢进化的结果，微生物生物体获得使用来自纤维素材料的戊糖的生长能力，同时保持原始生长动力学，有机酸生产速率和使用己糖作为碳源的能力 。 本发明还公开了赋予在生产商业上大量有机酸中同时使用己糖和戊糖的能力的微生物中的遗传变化。
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