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    Buffering system and its use in electrophoretic processes
    1.
    发明授权
    Buffering system and its use in electrophoretic processes 失效
    缓冲系统及其在电泳过程中的应用

    公开(公告)号：US5447612A

    公开(公告)日：1995-09-05

    申请号：US363640

    申请日：1994-12-22

    申请人： Milan Bier ,  Terry D. Long ,  Rodolfo B. Marguez ,  James A. Ostrem

    发明人： Milan Bier ,  Terry D. Long ,  Rodolfo B. Marguez ,  James A. Ostrem

    IPC分类号： G01N27/447 ,  C25B7/00

    CPC分类号： G01N27/44795 ,  G01N27/44747

    摘要： A novel pH buffering system which is functionally stable under electrophoretic conditions employs buffering components in complementary buffer pairs. The buffering components are selected from among simple chemically defined ampholytes, weak acids and weak bases, and are paired together on the basis of their dissociation characteristics to define narrow overlapping buffering zones in which the buffering components exhibit low net electrophoretic mobility combined with desired pH buffering capacity. The narrow buffering zones cover from 0.4 to 1.25 pH units within a broad range of pH values of from about pH 3 to about pH 10. Specific buffering pH values within the narrow buffering zones are obtainable by varying the molar ratios of the two buffering components of the selected buffer pair. The buffering system may be used to form functionally stable precast narrow pH zone gradients in free solution, and has particular utility in isoelectric focusing and other electrophoretic processes.

    摘要翻译： 在电泳条件下功能稳定的新型pH缓冲系统使用互补缓冲液对中的缓冲组分。 缓冲组分选自简单的化学定义的两性电解质，弱酸和弱碱，并且基于它们的解离特性配对在一起，以限定窄的重叠缓冲区，其中缓冲组分表现出低的电泳迁移率与期望的pH缓冲相结合 容量。 窄的缓冲区域在pH范围为约pH 3至约pH 10的范围内，覆盖0.4至1.25个pH单位。窄缓冲区域内的特定缓冲pH值可通过改变两个缓冲组分的摩尔比来获得 所选择的缓冲区对。 缓冲系统可用于在游离溶液中形成功能稳定的预制窄pH区梯度，并且在等电聚焦和其他电泳过程中具有特殊的用途。

    Multi-channel separation device
    2.
    发明授权
    Multi-channel separation device 失效
    多通道分离装置

    公开(公告)号：US5540826A

    公开(公告)日：1996-07-30

    申请号：US403982

    申请日：1995-03-15

    申请人： Milan Bier ,  Terry D. Long ,  Rodolfo Marquez ,  Anthony R. Ford

    发明人： Milan Bier ,  Terry D. Long ,  Rodolfo Marquez ,  Anthony R. Ford

    IPC分类号： B01D35/06 ,  B01D61/46 ,  B01D63/08 ,  G01N27/447 ,  B01D61/42

    CPC分类号： G01N27/44795 ,  B01D35/06 ,  B01D61/46 ,  B01D63/087

    摘要： A multi-channel separation device is provided for use in separation technology, particularly in electrophoresis and in isoelectric focusing. The device includes two mating plates, at least one of which contains an array of at least three cavities arranged to define a serpentine fluid pathway through the cavities when the plates are mated. Each of the cavities includes fluid input and output ports to facilitate transfer of process fluid through the cavities. A septum is disposed between the plates for streamlining fluid flow between the input and output ports by restricting but not preventing free fluid flow between adjacent cavities. The device also includes anodic and cathodic compartments containing electrodes for establishing an electric field across the cavities.

    摘要翻译： 提供用于分离技术的多通道分离装置，特别是在电泳和等电聚焦中。 该装置包括两个配合板，其中至少一个包含至少三个空腔的阵列，其布置成当板配合时限定通过空腔的蛇形流体路径。 每个空腔包括流体输入和输出端口，以便于工艺流体通过空腔的传送。 隔板设置在板之间，用于通过限制但不防止相邻空腔之间的自由流体流动来简化输入和输出端口之间的流体流动。 该装置还包括阳极和阴极室，其包含用于在空腔之间建立电场的电极。