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1.发明授权Insulin-secreting immortalized liver cell line modified by glucose sensitivity 失效通过葡萄糖敏感性修饰的胰岛素分泌永生化肝细胞系公开(公告)号：US07312077B2
公开(公告)日：2007-12-25
申请号：US10432592
申请日：2002-10-02
申请人： Naoya Kobayashi , Noriaki Tanaka , Teru Okitsu , Ji-Won Yoon , Hee-Sook Jun , Seungjin Shin
发明人： Naoya Kobayashi , Noriaki Tanaka , Teru Okitsu , Ji-Won Yoon , Hee-Sook Jun , Seungjin Shin
CPC分类号： C12N15/907 , A61K35/12 , A61K48/00 , C07K14/62 , C12N5/067 , C12N15/63 , C12N2501/70 , C12N2510/00 , C12N2510/04
摘要： The present invention provides a cell line which can be substituted for β cells in human mature pancreatic islets and express insulin in a glucose-concentration dependent manner, and enables the easy obtainment of the number of cells which meets the demand. The present invention also provides a therapeutical cell preparation for treating diabetes. The cell lines of the present invention can be obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human immortalized hepatic cell line FERM BP-7498 containing the TERT gene inserted in between a pair of LoxP sequences.
摘要翻译： 本发明提供了可以在人成熟胰岛中代替β细胞并以葡萄糖浓度依赖性方式表达胰岛素的细胞系，并且能够容易地获得满足需求的细胞数量。 本发明还提供了治疗糖尿病的治疗细胞制剂。 本发明的细胞系可以通过将编码他莫昔芬诱导的Cre重组酶的核苷酸序列与编码由葡萄糖敏感性启动子调节的胰岛素的核苷酸序列整合到人永生化肝细胞系FERM BP-7498中而获得，所述人类永生化肝细胞系FERM BP-7498含有 TERT基因插入一对LoxP序列之间。
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2.发明授权Fluorescent hydrogel and method for producing the same, and sensor for measuring saccharides using the same 有权荧光水凝胶及其制造方法以及使用该水凝胶测定糖的传感器公开(公告)号：US08822722B2
公开(公告)日：2014-09-02
申请号：US13877004
申请日：2011-09-07
申请人： Yun-Jung Heo , Hideaki Shibata , Tetsuro Kawanishi , Shoji Takeuchi , Yukiko Matsunaga , Teru Okitsu
发明人： Yun-Jung Heo , Hideaki Shibata , Tetsuro Kawanishi , Shoji Takeuchi , Yukiko Matsunaga , Teru Okitsu
IPC分类号： C07F5/02 , C08F222/00 , G01N33/53 , C09K11/06 , A61B5/145 , G01N21/64 , C08F220/54 , A61B5/00 , C08F220/36 , G01N21/77
CPC分类号： A61B5/0071 , A61B5/14532 , C08F220/36 , C08F220/54 , C08F290/062 , G01N21/6428 , G01N2021/7786 , Y10S436/80
摘要： To provide a fluorescent hydrogel having superior detectability of saccharides such as glucose and minimal invasiveness, a method for producing the same, and a sensor for measuring saccharides using the same. A florescent hydrogel having a structure represented by the following chemical formula 1, a method for producing the same, and a sensor for measuring saccharides using the same.
摘要翻译： 为了提供具有优异的糖类如葡萄糖的可检测性和最小的侵入性的荧光水凝胶，其制造方法和使用该荧光测定糖的传感器。 具有由以下化学式1表示的结构的荧光水凝胶，其制造方法和使用其的糖类测定用传感器。
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3.发明申请FLUORESCENT HYDROGEL AND METHOD FOR PRODUCING THE SAME, AND SENSOR FOR MEASURING SACCHARIDES USING THE SAME 有权荧光水凝胶及其制造方法，以及使用其测量酸度的传感器公开(公告)号：US20130267800A1
公开(公告)日：2013-10-10
申请号：US13877004
申请日：2011-09-07
申请人： Yun-Jung Heo , Hideaki Shibata , Tetsuro Kawanishi , Shoji Takeuchi , Yukiko Matsunaga , Teru Okitsu
发明人： Yun-Jung Heo , Hideaki Shibata , Tetsuro Kawanishi , Shoji Takeuchi , Yukiko Matsunaga , Teru Okitsu
CPC分类号： A61B5/0071 , A61B5/14532 , C08F220/36 , C08F220/54 , C08F290/062 , G01N21/6428 , G01N2021/7786 , Y10S436/80
摘要： To provide a fluorescent hydrogel having superior detectability of saccharides such as glucose and minimal invasiveness, a method for producing the same, and a sensor for measuring saccharides using the same. A florescent hydrogel having a structure represented by the following chemical formula 1, a method for producing the same, and a sensor for measuring saccharides using the same.
摘要翻译： 为了提供具有优异的糖类如葡萄糖的可检测性和最小的侵入性的荧光水凝胶，其制造方法和使用该荧光测定糖的传感器。 具有由以下化学式1表示的结构的荧光水凝胶，其制造方法和使用其的糖类测定用传感器。
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4.发明授权Immortalized hepatocyte cell line secreting modified insulin with glucose sensitivity 失效具有葡萄糖敏感性的永生化肝细胞细胞系分泌修饰胰岛素公开(公告)号：US07919080B2
公开(公告)日：2011-04-05
申请号：US11984006
申请日：2007-11-13
申请人： Naoya Kobayashi , Noriaki Tanaka , Teru Okitsu , Ji-Won Yoon , Hee-Sook Jun , Seungjin Shin
发明人： Naoya Kobayashi , Noriaki Tanaka , Teru Okitsu , Ji-Won Yoon , Hee-Sook Jun , Seungjin Shin
CPC分类号： C12N15/907 , A61K35/12 , A61K48/00 , C07K14/62 , C12N5/067 , C12N15/63 , C12N2501/70 , C12N2510/00 , C12N2510/04
摘要： The present invention provides a cell line which can be substituted for β cells in human mature pancreatic islets and express insulin in a glucose-concentration dependent manner, and enables the easy obtainment of the number of cells which meets the demand. The present invention also provides a therapeutical cell preparation for treating diabetes. The cell lines of the present invention can be obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human immortalized hepatic cell line FERM BP-7498 containing the TERT gene inserted in between a pair of LoxP sequences.
摘要翻译： 本发明提供可以代替“bgr”的细胞系。 人成熟胰岛中的细胞并以葡萄糖浓度依赖的方式表达胰岛素，并且能够容易地获得满足需求的细胞数量。 本发明还提供了治疗糖尿病的治疗细胞制剂。 本发明的细胞系可以通过将编码他莫昔芬诱导的Cre重组酶的核苷酸序列与编码由葡萄糖敏感性启动子调节的胰岛素的核苷酸序列整合到人永生化肝细胞系FERM BP-7498中而获得，所述人类永生化肝细胞系FERM BP-7498含有 TERT基因插入一对LoxP序列之间。
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5.发明申请Immortalized hepatocyte cell line secreting modified insulin with glucose sensitivity 失效具有葡萄糖敏感性的永生化肝细胞细胞系分泌修饰胰岛素公开(公告)号：US20090209033A1
公开(公告)日：2009-08-20
申请号：US11984006
申请日：2007-11-13
申请人： Naoya Kobayashi , Noriaki Tanaka , Teru Okitsu , Ji-Won Yoon , Hee-Sook Jun , Seungjin Shin
发明人： Naoya Kobayashi , Noriaki Tanaka , Teru Okitsu , Ji-Won Yoon , Hee-Sook Jun , Seungjin Shin
CPC分类号： C12N15/907 , A61K35/12 , A61K48/00 , C07K14/62 , C12N5/067 , C12N15/63 , C12N2501/70 , C12N2510/00 , C12N2510/04
摘要： The present invention provides a cell line which can be substituted for β cells in human mature pancreatic islets and express insulin in a glucose-concentration dependent manner, and enables the easy obtainment of the number of cells which meets the demand. The present invention also provides a therapeutical cell preparation for treating diabetes. The cell lines of the present invention can be obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human immortalized hepatic cell line FERM BP-7498 containing the TERT gene inserted in between a pair of LoxP sequences.
摘要翻译： 本发明提供了可以在人成熟胰岛中代替β细胞并以葡萄糖浓度依赖性方式表达胰岛素的细胞系，并且能够容易地获得满足需求的细胞数量。 本发明还提供了治疗糖尿病的治疗细胞制剂。 本发明的细胞系可以通过将编码他莫昔芬诱导的Cre重组酶的核苷酸序列与编码由葡萄糖敏感性启动子调节的胰岛素的核苷酸序列整合到人永生化肝细胞系FERM BP-7498中而获得，所述人类永生化肝细胞系FERM BP-7498含有 TERT基因插入一对LoxP序列之间。
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