公开(公告)号：US20060088859A1

公开(公告)日：2006-04-27

申请号：US11226605

申请日：2005-09-14

申请人： Thomas Hosted ,  Scott Walker ,  Marvin Bayne

发明人： Thomas Hosted ,  Scott Walker ,  Marvin Bayne

IPC分类号： C12Q1/68 ,  C12Q1/18 ,  C12N1/18 ,  C12N15/74

CPC分类号： C12Q1/18 ,  C07K14/395 ,  C12Q1/6897 ,  C12Q2600/158

摘要： The present invention comprises responsive promoters along with screening methods which make use of the promoters in order to identify anti-fungal substances.

摘要翻译： 本发明包括响应性启动子以及利用启动子以筛选抗真菌物质的筛选方法。

公开(公告)号：US20050233414A1

公开(公告)日：2005-10-20

申请号：US11021825

申请日：2004-12-23

申请人： Thomas Hosted ,  Tim Wang ,  Ann Horan

发明人： Thomas Hosted ,  Tim Wang ,  Ann Horan

IPC分类号： C12N1/21 ,  C12N9/22 ,  C12N15/52 ,  C12N15/76 ,  C12N15/90 ,  C12P19/04 ,  C12P19/60 ,  C12P21/06 ,  C07H21/04 ,  C12N9/10 ,  C12N15/74

CPC分类号： C12N9/22 ,  C12N15/52 ,  C12N15/76 ,  C12N15/90 ,  C12N2800/30 ,  C12P19/04 ,  C12P19/60

摘要： This invention is directed to nucleic acids which encode the proteins that direct the synthesis of the orthosomycin everninomicin and to use of the nucleic acids and proteins to produce compounds exhibiting antibiotic activity based on the everninomycin structure. The DNA sequence for the gene clusters responsible for encoding everninomicin biosynthetic genes, which provide the machinery for producing everninomicin, are provided. Thus, this invention provides the nucleic acid sequences needed to synthesize novel everninomicin-related compounds based on everninomicin, arising from modifications of the DNA sequence designed to change glycosyl and modified orsellinic acid groups contained in everninomicin. A Micromonospora site-specific integrase gene is also provided, which can be incorporated in a vector for integration into any actinomycete, and, particularly into Monospora. Thus, the invention further provides methods for introducing heterologous genes into an actinomycete chromosome using this particular vector.

摘要翻译： 本发明涉及编码引导正交霉素埃芬诺米星的合成的蛋白质的核酸，以及使用核酸和蛋白质产生基于依维诺霉素结构显示抗生素活性的化合物的核酸。 提供了负责编码everninomicin生物合成基因的基因簇的DNA序列，其提供了生产everninomicin的机制。 因此，本发明提供了合成基于everninomicin的新型everninomicin相关化合物所需的核酸序列，其由设计用于改变everninomicin中所含的糖基和修饰的orsellinic酸基团的DNA序列的修饰产生。 还提供了一个小单孢菌属位点特异性整合酶基因，其可以并入载体中以整合到任何放线菌，特别是单孢菌素中。 因此，本发明还提供了使用该特定载体将异源基因引入放线菌染色体的方法。