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    METHOD FOR PRODUCING REGENERATED T CELL POPULATION VIA iPS CELLS
    1.
    发明申请
    METHOD FOR PRODUCING REGENERATED T CELL POPULATION VIA iPS CELLS 有权

    公开(公告)号：US20210238550A1

    公开(公告)日：2021-08-05

    申请号：US17264485

    申请日：2019-07-30

    申请人： Thyas Co. Ltd.

    发明人： Yutaka Yasui ,  Yasumichi Hitoshi ,  Hiroyuki Ueno ,  Yoshimoto Katsura ,  Shin Kaneko

    IPC分类号： C12N5/0783 ,  A61K35/17

    摘要： The present invention provides a method for producing a regenerated T cell population via iPS cells, wherein the said regenerated T cell population maintains the repertoire diversity of a T cell population before initialization and has specificity to an antigen. The method comprises steps of (1) bringing a T cell population into contact with one or more tumor-related antigens, or a biological tissue containing one or more tumor-related antigens, or a crushed material or a lysate thereof, and enriching the T cell population having specificity to the tumor-related antigens, (2) initializing the enriched T cell population to iPS cells, and culturing while maintaining the repertoire diversity of the enriched T cell population, and (3) producing a regenerated T cell population from the cultured iPS cells.

    METHOD FOR PRODUCING REGENERATED T CELL VIA IPS CELL
    3.
    发明公开
    METHOD FOR PRODUCING REGENERATED T CELL VIA IPS CELL 审中-公开

    公开(公告)号：US20240052309A1

    公开(公告)日：2024-02-15

    申请号：US18259687

    申请日：2021-12-29

    申请人： THYAS CO. LTD. ,  KOTAI BIOTECHNOLOGIES, INC.

    发明人： Shin Kaneko ,  Ryosuke Gonotsubo ,  Mitsujiro Osawa ,  Yasumichi Hitoshi ,  Kazuo Yamashita ,  Nicolas Claude Paul Sax

    IPC分类号： C12N5/0783 ,  A61P35/00 ,  C07K14/725 ,  C12N9/22 ,  A01N1/02

    CPC分类号： C12N5/0636 ,  A61P35/00 ,  C07K14/7051 ,  C12N9/22 ,  A01N1/0284 ,  C12N2506/45 ,  C12N2310/20 ,  C12N2506/115

    摘要： [Problems]
    To provide a method for producing regenerated T cells via iPS cells into which TCRs isolated from tumor tissue-infiltrating CD106-positive T cells have been introduced.
    [Solutions]
    Provided is a method for producing regenerated T cells via iPS cells, comprising: 1. A step for preparing cDNAs respectively encoding a TCR α chain and a TCR β chain from individual cells in a CD106-positive T cell population obtained from subjects and having reactivity with a tumor-related antigen; 2. A step for reprogramming peripheral blood mononuclear cells which are obtained from the subjects and from which B cells and T cells have been removed or T cells into iPS cells, and selecting iPS cell clones having high efficiency of differentiation into T cells from the obtained iPS cells; 3. A step for introducing the cDNAs into the iPS cell clones, hematopoietic stem cells differentiated from the iPS cell clones, immature T cells differentiated from the hematopoietic stem cells, or mature T cells differentiated from the immature T cells; and 4. A step for performing the differentiation of the iPS cell clones having the cDNAs introduced therein, the hematopoietic stem cells or the immature T cells which have been obtained in step 3 into mature T cells and the proliferation of the mature T cells.

    METHOD FOR PRODUCING REGENERATED T CELLS VIA IPS CELLS
    5.
    发明公开
    METHOD FOR PRODUCING REGENERATED T CELLS VIA IPS CELLS 审中-公开

    公开(公告)号：US20230183645A1

    公开(公告)日：2023-06-15

    申请号：US17924480

    申请日：2021-09-17

    申请人： Thyas Co. Ltd

    发明人： Shin Kaneko ,  Kyosuke Gonotsubo ,  Mitsujiro Osawa ,  Yasumichi Hitoshi

    IPC分类号： C12N5/0783 ,  A61P35/00 ,  C12N15/63

    CPC分类号： C12N5/0636 ,  A61P35/00 ,  C12N15/63 ,  C12N2506/45

    摘要： [Problem]
    To provide a method for producing regenerated T cells by introducing antigen-specific T cell receptors to iPS cell clones or hematopoietic stem cells differentiated from iPS cell clones, immature T cells or mature T cells.
    [Solution]
    The present invention is a method for producing regenerated T cells via iPS cells, the method comprising: (1) a step for obtaining T cells from a subject, and preparing, from individual cells in T cell population that is reactive to a tumor-related antigen, cDNAs that code for TCR α chain and TCR β chain; (2) a step for initializing a subject-derived peripheral blood mononuclear cells from which B cells and T cells have been removed, or T cells, to iPS cells, and selecting, from the iPS cells thus obtained, iPS cell clones that demonstrate high differentiation efficiency into T cells; (3) a step for introducing the cDNAs from the individual cells into the iPS cell clones, into hematopoietic stem cells differentiated from the iPS cell clones, into immature T cells differentiated from the hematopoietic stem cells, or into mature T cells differentiated from the immature T cells; and (4) a step for differentiating the iPS cell clones, the hematopoietic stem cells or the immature T cells, which are obtained in step (3) and into which the cDNAs have been introduced, into mature T cells and proliferating the mature T cells.