Method for introducing substance into plant

    公开(公告)号:US11549120B2

    公开(公告)日:2023-01-10

    申请号:US16089813

    申请日:2017-03-28

    摘要: The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises the steps of: obtaining an enzymatically treated and isolated fertilized egg cell by (1-i) isolating a fertilized egg cell from a plant tissue containing a fertilized egg cell, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, (1-ii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the fertilized egg cell that has been enzymatically treated, (1-iii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and simultaneously isolating the fertilized egg cell that has been enzymatically treated, (1-iv) isolating an egg cell and a sperm cell from a plant to produce a fertilized egg by fusing the cells, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, or (1-v) treating a plant tissue containing an egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the egg cell that has been enzymatically treated, and further fusing the egg cell with an isolated sperm cell; and (2) introducing a substance selected from the group consisting of nucleic acids, proteins, and peptides into the resultant enzymatically treated and isolated fertilized egg cell.

    OUTPUT VOLTAGE CONTROL CIRCUIT DEVICE FOR PLURALITY OF POWER SUPPLY CELLS CONNECTED IN SERIES

    公开(公告)号:US20200321482A1

    公开(公告)日:2020-10-08

    申请号:US16823896

    申请日:2020-03-19

    摘要: An output voltage control circuit device includes a rectifying unit configured to permit a flow of a current from a negative electrode side to a positive electrode side of a power supply cell, a voltage generation unit, through which a current is able to flow in both directions between both ends inserted between a negative electrode of an odd-numbered power supply cell counted from the negative electrode side of the power supply cells and an anode of the rectifying unit, and a generated voltage between both ends is able to be cyclically inverted; a voltage holding capacitor, and a voltage control circuit unit configured to generate a voltage, a direction of which is cyclically inverted, between both ends of the voltage generation unit on the negative electrode side of the power supply cells.

    Atomic oscillator
    4.
    发明授权

    公开(公告)号:US10720745B2

    公开(公告)日:2020-07-21

    申请号:US16331910

    申请日:2017-08-29

    发明人: Shigeyoshi Goka

    摘要: The present invention provides an atomic oscillator comprising: a first frequency synthesizer for multiplying a reference frequency signal oscillated with a first voltage control oscillator by a first multiplication ratio and synthesizing a first high frequency signal; a first semiconductor laser for emitting a first laser light modulated by the first high frequency signal; a second frequency synthesizer for multiplying an output signal oscillated by a second voltage control oscillator with a second multiplication ratio and synthesizing a second high frequency signal; a second semiconductor laser for emitting a second laser modulated by the second high frequency signal; a gas cell being emitted with the first laser light and the second laser light simultaneously, the gas cell enclosing a first alkali-metal atom gas, a second alkali-metal atom gas, and buffer gas; and a computer for setting the first multiplication ratio in the first frequency synthesizer.

    FINGER MECHANISM AND HUMANOID HAND INCORPORATING SAME FINGER MECHANISM

    公开(公告)号:US20200206950A1

    公开(公告)日:2020-07-02

    申请号:US16498926

    申请日:2018-03-20

    发明人: Naoki Fukaya

    IPC分类号: B25J15/00 B25J15/02

    摘要: A finger mechanism for a robot, an artificial hand, and the like, wherein a fourth bone member (14) of the bone members of the finger mechanism and corresponding to the distal phalanx comprises: a support portion (15) that is rotatably coupled to a third bone member corresponding to the middle phalanx by a rotational shaft (g5); and a nail portion (16). The nail portion (16) can freely rotate about a shaft (g7) at a right angle or a near right angle to the rotational shaft (g5), and a return mechanism (17) to return the rotated nail portion (16) to a reference position is provided between the support portion (15) and the nail portion (16). In this manner, in response to the amount of force applied to the fourth bone member (14), it is possible for only the nail portion (16) to rotate in a direction to easily grasp an object to be held.

    Method for manufacturing secondary battery separator and method for manufacturing lithium secondary battery

    公开(公告)号:US10096809B2

    公开(公告)日:2018-10-09

    申请号:US14895389

    申请日:2014-06-04

    摘要: A method for manufacturing, a secondary battery separator including a porous resin film in which pores have three-dimensionally ordered structure and are in mutual communication via through-holes. The method includes: uniformly dispersing spherical microparticles having narrow particle size distribution in a dispersion medium to prepare a microparticles-dispersed slurry; drying slurry to obtain a spherical microparticles-dispersed film; heat-treating the film to form a microparticles-resin film in which the microparticles are regularly arrayed in three-dimensions in a resin matrix; and contacting the microparticles-resin film with an organic acid, water, an alkaline solution or an inorganic acid other than hydrofluoric acid to dissolve and remove the microparticles, or heating the microparticles-resin film to remove the microparticles, to form pores which are in mutual communication and regularly arrayed in the resin matrix. The medium has a resin precursor for the resin matrix and surfaces of the microparticles are inactive against the medium.