Hydrogel-enzyme systems and methods

    公开(公告)号:US12098272B2

    公开(公告)日:2024-09-24

    申请号:US17397967

    申请日:2021-08-09

    IPC分类号: C08L33/10 C12N9/16

    摘要: In one embodiment, a hydrogel-enzyme construct for performing high temperature enzymatic reaction on paraoxon, and/or for performing enzymatic reaction on paraoxon following exposure to high temperature, includes a hydrogel having multiple layers of poly(methacrylic acid) (PMAA) and a plurality of dPTE2 enzyme molecules. Individual dPTE2 enzyme molecules are embedded between adjacent PMAA layers and are covalently bonded with respective individual PMAA layers. The hydrogel-enzyme construct is capable of performing enzymatic reaction on the paraoxon when the paraoxon is exposed to the hydrogel-enzyme construct under a temperature condition of up to above 99° C. and below 100° C. or when the paraoxon is exposed to the hydrogel-enzyme construct after the hydrogel-enzyme construct has been heated to a temperature condition of up to 550° C., where the enzymatic reaction on the paraoxon by individual dPTE2 molecules embedded within the hydrogel occurs at a residual activity of between 20% and 100%.

    Modular bathymetry systems and methods

    公开(公告)号:US12046150B2

    公开(公告)日:2024-07-23

    申请号:US17039777

    申请日:2020-09-30

    IPC分类号: G09B23/12

    CPC分类号: G09B23/12

    摘要: In one embodiment, a bathymetry system includes a base slab, multiple spacer assemblies, a decking layer, multiple computer numerical control routed blocks, a waterproof coating, and multiple floor joints. The spacer assemblies are coupled with a top surface of the base slab, the decking layer is disposed on top surfaces of the spacer assemblies, the blocks are disposed on a top surface of the decking layer, floor joints are disposed between blocks and the decking layer, and the waterproof coating is disposed on top surfaces of the blocks. The top surfaces of the routed blocks are shaped so that the blocks collectively provide a bathymetry profile, such as a bottom contour of a riverbed.