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1.发明授权Plant cell culture for production of natural products with reduced glucosinolate contamination 失效用于生产具有降低的硫代葡萄糖苷污染的天然产物的植物细胞培养物公开(公告)号：US08642340B2
公开(公告)日：2014-02-04
申请号：US13140381
申请日：2009-12-17
IPC分类号： C12N5/00
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译： 本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。 所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。 该方法还可以包括从培养物中回收天然产物。 还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。 该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。 该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。 最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。
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2.发明申请PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED GLUCOSINOLATE CONTAMINATION 失效用于生产具有降低的葡萄糖苷酸盐污染的天然产品的植物细胞培养物公开(公告)号：US20110251408A1
公开(公告)日：2011-10-13
申请号：US13140381
申请日：2009-12-17
IPC分类号： C07D311/60 , C12P17/06
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译： 本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。 所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。 该方法还可以包括从培养物中回收天然产物。 还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。 该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。 该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。 最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。
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3.发明申请公开(公告)号：US20090007298A1
公开(公告)日：2009-01-01
申请号：US12160066
申请日：2007-01-03
CPC分类号： C12N15/8205
摘要： Reported herein are methods for obtaining stably transformed callus in Taxus media ‘Hicksii’, including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in Taxus media cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., Taxus plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.
摘要翻译： 本文报道的是在紫杉属培养基“Hicksii”中获得稳定转化的愈伤组织的方法，特别涉及使用针，茎或树皮剥离作为转化的外植体材料的方法。 还提供了在Taxus培养基细胞，特别是悬浮培养物，愈伤组织和针头细胞中指导报道基因表达的若干启动子活性的描述。 还提供转基因植物（例如紫杉属植物），植物细胞，细胞系和组织（包括种子），特别是在紫杉醇生物合成途径中表达一种或多种酶的那些。
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公开(公告)号：US06222522B1
公开(公告)日：2001-04-24
申请号：US09156595
申请日：1998-09-18
申请人： Max V. Mathews , Thomas E. Oberheim
发明人： Max V. Mathews , Thomas E. Oberheim
IPC分类号： G09G500
CPC分类号： G06F3/044 , G10H1/00 , G10H2220/185 , G10H2220/401 , G10H2220/425
摘要： A position sensor is used in conjunction with one or more batons, each baton having a transmitter that transmits a distinct radio frequency signal at a position in space. The position sensor determines the current position of each baton transmitter in terms of X, Y, and Z coordinates. The position sensor includes a tablet having a flat support member, with at least two pairs of electrodes coupled to the flat support member. Each of the electrodes is a separate antenna. A first pair of the electrodes is shaped so that the amount of capacitive coupling between each baton transmitter and the first pair of electrodes corresponds to the position of the baton transmitter with respect to the X axis. A second pair of the electrodes is shaped so that the amount of capacitive coupling between each baton transmitter and the second pair of electrodes corresponds to the position of the baton transmitter with respect to the Y axis. The regions forming the first pair electrodes are preferably interleaved with the regions forming the second pair of electrodes. The X, Y position of the baton can be computed with uniform accuracy anywhere in the X-Y plane by measuring the capacitance values between the baton and the four antennae.
摘要翻译： 位置传感器与一个或多个警棍一起使用，每个警棍具有发射器，其在空间中的位置发射不同的射频信号。 位置传感器根据X，Y和Z坐标确定每个指挥发射机的当前位置。 位置传感器包括具有平坦支撑构件的片剂，其具有耦合到平坦支撑构件的至少两对电极。 每个电极是单独的天线。 第一对电极被成形为使得每个指挥棒发射器和第一对电极之间的电容耦合量对应于指挥发射机相对于X轴的位置。 第二对电极被成形为使得每个指挥棒发射器和第二对电极之间的电容耦合量对应于指挥发射机相对于Y轴的位置。 形成第一对电极的区域优选地与形成第二对电极的区域交错。 在X-Y平面的任何地方，可以通过测量指挥棒与四个天线之间的电容值，以均匀的精度计算指挥棒的X，Y位置。
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公开(公告)号：US5750870A
公开(公告)日：1998-05-12
申请号：US384556
申请日：1995-02-03
CPC分类号： C12N9/0071 , A01H4/001 , A01H4/008 , C12N15/8205 , C12N15/8209 , C12N15/8249 , C12N9/14 , C12Y114/17004
摘要： An efficient transformation system for plants has been developed that yields high transformation efficiencies and pure transgenic plants. Genomic integration of transgenes was confirmed by genomic DNA hybridization analysis. Pure transgenic plants have been successfully established in soil.
摘要翻译： 已经开发了一种有效的植物转化系统，其产生高转化效率和纯转基因植物。 通过基因组DNA杂交分析证实转基因的基因组整合。 在土壤中成功建立了纯转基因植物。
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6.发明申请IDENTIFICATION AND CHARACTERIZATION OF AN ANTHOCYANIN MUTANT (ANT1) IN TOMATO 审中-公开苯甲酰胺突变体（ANT1）在TOMATO中的鉴定和表征公开(公告)号：US20100016565A1
公开(公告)日：2010-01-21
申请号：US12534826
申请日：2009-08-03
IPC分类号： C07H1/06
CPC分类号： C07K14/415 , C12N15/8212 , C12N15/825
摘要： Flavonoids are obtained from plants that overexpress an ANT1 gene compared to wild-type plants. The plant may be a transgenic plant that contains a transformation vector that causes the overexpression of ANT1. Alternatively, the plant can be selectively bred to have an allele of or mutation in an endogenous ANT1 gene that causes the overexpression of ANT1 compared to plants lacking the allele or mutation.
摘要翻译： 与野生型植物相比，黄酮类化合物从过表达ANT1基因的植物获得。 植物可以是含有导致ANT1的过表达的转化载体的转基因植物。 或者，与缺乏等位基因或突变的植物相比，植物可以选择性繁殖以具有导致ANT1的过表达的内源性ANT1基因的等位基因或突变。
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7.发明授权Identification and characterization of an Anthocyanin mutant (ANT1) in tomato 失效番茄中花青素突变体（ANT1）的鉴定和表征公开(公告)号：US07304207B2
公开(公告)日：2007-12-04
申请号：US10407845
申请日：2003-04-04
CPC分类号： C12N15/825 , C07K14/415 , C12N15/8212
摘要： The present invention is directed to a novel plant phenotype, designated Anthocyanin 1 (ANT1), a nucleic acid sequence expressed in plants demonstrating the ANT1 phenotype and the corresponding amino acid sequence. Also provided are plant cells and plants that exhibit modified ANT1 expression.
摘要翻译： 本发明涉及一种称为花青素1（ANT1）的新型植物表型，在植物中表达的表达ANT1表型的核酸序列和相应的氨基酸序列。 还提供了表现出修饰的ANT1表达的植物细胞和植物。
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8.发明授权
公开(公告)号：US6127182A
公开(公告)日：2000-10-03
申请号：US177758
申请日：1998-10-23
CPC分类号： A01H4/001 , A01H4/005 , A01H4/008 , C12N15/8201
摘要： An efficient micropropagation system for fruit-bearing trees has been developed for rapid clonal multiplication of large numbers of shoots within a short time. The micropropagation system provides for transformation and vegetative propagation of fruit-bearing tree shoots to produce uniform populations of transgenic fruit-bearing trees.
摘要翻译： 已经开发了一种用于果树的有效微繁殖系统，用于在短时间内快速克隆大量的芽。 微繁殖系统为果实树枝的转化和营养繁殖提供了转基因果实树的均匀种群。
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9.发明授权
公开(公告)号：US6054635A
公开(公告)日：2000-04-25
申请号：US777147
申请日：1996-12-27
CPC分类号： C12N15/8216 , C12N15/8235
摘要： The present invention is directed to the identification and isolation of a promoter region from a raspberry genome. The promoter is operably linked, in a native raspberry genome, to the coding region of a raspberry E4 gene. The raspberry E4 gene promoter of the invention is capable of regulating moderate level, constitutive expression of a heterologous plant gene under its control. The invention is further directed to chimeric genes, cassette vectors, kits, transgenic plants, and methods employing a raspberry E4 promoter.
摘要翻译： 本发明涉及从覆盆子基因组鉴定和分离启动子区。 启动子在天然覆盆子基因组中可操作地连接到覆盆子E4基因的编码区。 本发明的覆盆子E4基因启动子能够调节其控制下的异源植物基因的中等程度的组成型表达。 本发明还涉及嵌合基因，盒载体，试剂盒，转基因植物和使用覆盆子E4启动子的方法。
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公开(公告)号：US4960015A
公开(公告)日：1990-10-02
申请号：US517742
申请日：1990-05-02
申请人： Charlie V. Mathews
发明人： Charlie V. Mathews
CPC分类号： B25G1/04 , B25B13/481 , B25B23/0021 , Y10T16/473
摘要： An extendable torque bar has a multi-sided rack member with teeth extending along a side, which fits within a tubular member whose bore has a geometric configuration corresponding to the rack member. The rack member has an adapter for engaging a socket for a nut or bolt, or the like. The tubular member has a second adapter at its rear end for engaging a tool or other driving mechanism. A pawl is supported by an annular member which is affixed to the tubular member. The annular member has an outer surface shaped like the frustum of a cone. The pawl fits through an opening in the annular member and is held in place and pivots about a pivot member projecting through the annular member. The teeth of the pawl contact the teeth of the rack member by extending through a slotted opening in the tubular member. When the pawl teeth are in contact with the rack teeth, the edge of the pawl opposite the side of the pawl containing the teeth coincides with the plane of the outer surface of the annular member. A sleeve fits over the pawl and annular member. The top inner surface of the sleeve corresponds to the outer surface of the annular member and envelops that outer surface. The sleeve is slidable along the tubular shaft and locks the bar when it is urged against the annular member by biasing means.
摘要翻译： 可延伸的扭矩杆具有多边齿条构件，其具有沿着一侧延伸的齿，所述齿配合在其孔的几何构型对应于齿条构件的管状构件中。 齿条构件具有用于接合用于螺母或螺栓的插座等的适配器。 管状构件在其后端具有第二适配器，用于接合工具或其它驱动机构。 棘爪由固定到管状构件的环形构件支撑。 环形构件具有与锥体的平截头体形状相似的外表面。 棘爪穿过环形构件中的开口并且被保持在适当位置并且围绕通过环形构件突出的枢转构件枢转。 棘爪的齿通过延伸穿过管状构件中的开槽开口而接触齿条构件的齿。 当爪齿与齿条齿接触时，棘爪的与包含齿的棘爪侧相反的边缘与环形构件的外表面的平面重合。 套筒套在棘爪和环形构件上。 套筒的顶部内表面对应于环形构件的外表面并包封该外表面。 套筒可沿着管状轴滑动，并通过偏置装置将其锁定在环形构件上。
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