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公开(公告)号:US06787368B1
公开(公告)日:2004-09-07
申请号:US09517597
申请日:2000-03-02
申请人: Wah Y. Wong , Heman Chao , Donald Segal , Jerry McElroy
发明人: Wah Y. Wong , Heman Chao , Donald Segal , Jerry McElroy
IPC分类号: G01N3353
CPC分类号: G01N33/54373 , G01N33/5438
摘要: A diagnostic method and device for use in detecting or quantitating an analyte present in a liquid sample. The method includes reacting an analyte-containing sample with reagents capable of generating a first coil-forming peptide in solution form. This peptide is then contacted with a biosensor whose detection surface has surface-bound molecules of a second, oppositely charged coil-forming peptide, under conditions effective to form a stable &agr;-helical coiled-coil heterodimer on the detection surface. The formation of the coiled-coil heterodimer produces a measurable change in biosensor signal, which is measured to detect the presence of or quantitate the amount of analyte in a sample. Also disclosed is a biosensor device for carrying out the reaction.
摘要翻译: 用于检测或定量存在于液体样品中的分析物的诊断方法和装置。 所述方法包括使含分析物的样品与能够以溶液形式产生第一螺旋形成肽的试剂反应。 然后在检测表面具有表面结合分子的第二个带相反电荷的线圈形成肽的生物传感器上,在有效地在检测表面上形成稳定的α-螺旋卷曲线圈异二聚体的条件下接触该肽。 螺旋线圈异二聚体的形成产生生物传感器信号的可测量的变化,其被测量以检测样品中分析物的量的存在或定量。 还公开了用于进行反应的生物传感器装置。
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公开(公告)号:US06300141B1
公开(公告)日:2001-10-09
申请号:US09518178
申请日:2000-03-02
申请人: Donald Segal , Heman Chao , Wah Y. Wong , Jerry McElroy
发明人: Donald Segal , Heman Chao , Wah Y. Wong , Jerry McElroy
IPC分类号: G01N33543
CPC分类号: G01N33/5438 , G01N33/54366
摘要: A diagnostic card device for use in detecting or quantitating an analyte present in a liquid sample, comprising a card substrate having a sample introduction region, a biosensor, and a sample-flow pathway communicating between the sample-introduction region and the biosensor, circuitry for generating an analyte-dependent electrical signal from the biosensor; and a signal-responsive element for recording such signal. In one embodiment, the biosensor includes a detection surface with surface-bound molecules of a first charged, coil-forming peptide capable of interacting with a second, oppositely charged coil-forming peptide to form a stable &agr;-helical coiled-coil heterodimer, where the binding of the second peptide to the first peptide, to form such heterodimer, is effective to measurably alter a signal generated by the biosensor. The sample-flow pathway contains diffusibly bound conjugate of the second coil-forming peptide and the analyte (or an analyte analog) and immobilized analyte-binding agent. The analyte in the liquid sample and the conjugate compete for binding with the immobilized analyte-binding agent. Unbound conjugate migrates by capillarity to the biosensor. Liquid sample containing conjugate migrates in the sample flow pathway by capillary action or is driven by a micro-pump. In another embodiment, the biosensor includes an electrode substrate coated with a high-dielectric hydrocarbon-chain monolayer, and having analyte-binding agent attached to the exposed monolayer surface. Binding of analyte to the monolayer-bound analyte-binding agent, and the resultant perturbation of the monolayer structure, causes ion-mediated electron flow across the monolayer.
摘要翻译: 一种用于检测或定量存在于液体样品中的分析物的诊断卡装置,包括具有样品引入区域的卡片基底,生物传感器和在样品导入区域和生物传感器之间连通的样品流动路径,用于 从所述生物传感器产生与分析物相关的电信号; 以及用于记录这种信号的信号响应元件。 在一个实施方案中,生物传感器包括具有能够与第二带相反电荷的卷曲形成肽相互作用以形成稳定的α-螺旋卷曲螺旋状异二聚体的第一带电荷的线圈形成肽的表面结合分子的检测表面,其中 第二肽与第一肽的结合以形成这种异源二聚体对于可测量地改变由生物传感器产生的信号是有效的。 样品流动路径包含第二个螺旋形成肽和分析物(或分析物类似物)和固定的分析物结合剂的扩散结合的缀合物。 液体样品中的分析物和缀合物竞争与固定的分析物结合剂结合。 未结合的共轭体通过生物传感器的毛细作用迁移。 含有共轭的液体样品通过毛细管作用在样品流动路径中迁移或由微型泵驱动。 在另一个实施方案中,生物传感器包括涂覆有高电介质烃链单层的电极基底,并且具有附着到暴露的单层表面的分析物结合剂。 分析物与单层结合的分析物结合剂的结合以及由此导致的单层结构的扰动引起跨越单层的离子介导的电子流。
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公开(公告)号:US5738996A
公开(公告)日:1998-04-14
申请号:US260199
申请日:1994-06-15
申请人: Robert S. Hodges , Randall T. Irvin , Arne Holm , Wah Y. Wong , Hasmukh B. Sheth , Devon L. Husband
发明人: Robert S. Hodges , Randall T. Irvin , Arne Holm , Wah Y. Wong , Hasmukh B. Sheth , Devon L. Husband
CPC分类号: C40B80/00 , B01J19/0046 , C07K1/047 , G01N33/53 , B01J2219/00315 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要: A combinatorial library composition and method for using the library to construct oligomers effective to bind to a selected ligand is disclosed. The library composition includes at least two sets of ar oligomer libraries, each library set having selected oligomer subunit positions filled by known subunits, and other subunit positions containing permutations of subunits. In the selection method, oligomers from each library set are identified, and a new permutation library formed of subunits corresponding to the highest binding affinity oligomers in each library is screened for binding affinity to the selected ligand.
摘要翻译: 公开了组合文库组合物和使用文库构建有效结合选定配体的低聚物的方法。 文库组合物包括至少两组ar寡聚体文库,每个文库集合具有由已知亚基填充的选定的寡聚体亚单位,以及含有亚基排列的其它亚基位置。 在选择方法中,鉴定了来自每个文库的寡聚体,并筛选了与每个文库中最高结合亲和性寡聚体相应的亚基形成的新的置换文库,以对所选择的配体进行结合亲和力。
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公开(公告)号:US07264800B2
公开(公告)日:2007-09-04
申请号:US11046271
申请日:2005-01-27
申请人: Donald Segal , Jerry McElroy , Heman Chao , Wah Y. Wong , John Docherty , Jodi Dickstein
发明人: Donald Segal , Jerry McElroy , Heman Chao , Wah Y. Wong , John Docherty , Jodi Dickstein
CPC分类号: B82Y5/00 , A61K38/50 , A61K47/66 , A61K47/6815 , A61K47/6845 , A61K47/6851 , A61K47/6891 , A61K48/00
摘要: Improvements in methods of treating cancer with weakly basic anti-cancer compounds are provided. In one aspect, the invention provides an improvement in a method of treating cancer cells whose extracellular environment contains 1–8 mM urea, by exposing the cells to a weakly basic anti-cancer compound which is effective in inhibiting the growth of the cells. The improvement includes (a) exposing the cells to a urease enzyme composition and, (b) by step (a), reducing the amount of anti-cancer compound required to produce a given extent of inhibition in the growth of the cells when the cells are exposed to the anti-cancer agent. Methods of potentiating the specific therapeutic activity of a weakly basic anti-cancer compound in the treatment of a given mammalian cancer which is responsive to the compound are provided as are pharmaceutical compositions for use in intravenous administration to a subject are also provided.
摘要翻译: 提供了用弱碱性抗癌化合物治疗癌症的方法的改进。 一方面,本发明提供一种通过将细胞暴露于有效抑制细胞生长的弱碱性抗癌化合物来治疗细胞外环境含有1-8mM尿素的癌细胞的方法的改进。 改进包括(a)将细胞暴露于脲酶组合物,和(b)通过步骤(a),减少当细胞生长时产生给定程度的抑制所需的抗癌化合物的量 暴露于抗癌剂中。 提供了弱化碱性抗癌化合物在治疗对化合物有反应性的给定哺乳动物癌症中的特异性治疗活性的方法,也用于静脉内施用于受试者的药物组合物。
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公开(公告)号:US06767545B2
公开(公告)日:2004-07-27
申请号:US09865159
申请日:2001-05-24
申请人: Randall T. Irvin , Randy J. Read , Bart Hazes , Wah Y. Wong , Sastry A. Parimi , Linda M. G. Glasier
发明人: Randall T. Irvin , Randy J. Read , Bart Hazes , Wah Y. Wong , Sastry A. Parimi , Linda M. G. Glasier
IPC分类号: A61K3902
CPC分类号: A61K38/164 , C07K14/21 , C07K2319/00
摘要: A composition and method for treating or preventing infection by Pseudomonas aeruginosa is disclosed. The composition includes a P. aeruginosa pilin peptide modified to prevent oligomerization of the pilin. The method involves administered the composition to a person infected with Pseudomonas are at risk of such infection.
摘要翻译: 公开了一种用于治疗或预防铜绿假单胞菌感染的组合物和方法。 该组合物包括经改良的铜绿假单胞菌肽蛋白肽,以防止皮林的低聚。 该方法包括将组合物给予感染了假单胞菌的人患有这种感染的风险。
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公开(公告)号:US5494672A
公开(公告)日:1996-02-27
申请号:US638492
申请日:1991-01-04
申请人: Robert S. Hodges , William Paranchych , Randall T. Irvin , Kok K. Lee , Sastry A. Parimi , Dick E. Zoutman , Peter C. Doig , Wah Y. Wong
发明人: Robert S. Hodges , William Paranchych , Randall T. Irvin , Kok K. Lee , Sastry A. Parimi , Dick E. Zoutman , Peter C. Doig , Wah Y. Wong
IPC分类号: A61K39/00 , A61K31/00 , A61K38/00 , A61K39/104 , A61K39/395 , A61P31/00 , A61P31/04 , C07K7/08 , C07K14/195 , C07K14/21 , C07K14/41 , C07K14/705 , C07K16/00 , C07K16/46 , C07K19/00 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/385 , G01N33/53 , G01N33/569 , A61K38/04 , A61K39/02
CPC分类号: C07K16/461 , C07K14/21 , A61K38/00 , A61K39/00 , C07K2319/00
摘要: A peptide having a sequence corresponding to a C-terminal portion of the Pseudomonas aeruginosa pilin protein is disclosed. The peptide is cross-reactive with surface peptides present in certain bacterial and fungal microorganisms, and is effective in inhibiting binding of such organisms to target epithelial cells. The peptide may also be employed in a vaccine composition, for producing immunity against such cross-reactive microorganisms. Also disclosed are methods of preparing peptides which are cross-reactive with the P. aeruginosa pilin peptide, and chimeric monoclonal antibodies immunoreactive with the pilin peptide.
摘要翻译: 公开了具有对应于铜绿假单胞菌毛林蛋白蛋白的C末端部分的序列的肽。 该肽与某些细菌和真菌微生物中存在的表面肽具有交叉反应性,并且有效抑制这些生物体与目标上皮细胞的结合。 肽也可以用于疫苗组合物中,用于产生针对这种交叉反应性微生物的免疫。 还公开了制备与铜绿假单胞菌菌素肽交叉反应的肽的方法和与肽素肽免疫反应的嵌合单克隆抗体。
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公开(公告)号:US06342233B1
公开(公告)日:2002-01-29
申请号:US09329884
申请日:1999-06-11
申请人: Randall T. Irvin , Randy J. Read , Bart Hazes , Wah Y. Wong , Sastry A. Parimi , Linda M. G. Glasier
发明人: Randall T. Irvin , Randy J. Read , Bart Hazes , Wah Y. Wong , Sastry A. Parimi , Linda M. G. Glasier
IPC分类号: A61K39108
CPC分类号: A61K38/164 , C07K14/21 , C07K2319/00
摘要: A composition and method for treating or preventing infection by Pseudomonas aeruginosa is disclosed. The composition includes a P. aeruginosa pilin peptide modified to prevent oligomerization of the pilin. The method involves administered the composition to a person infected with Pseudomonas are at risk of such infection.
摘要翻译: 公开了一种用于治疗或预防铜绿假单胞菌感染的组合物和方法。 该组合物包括经改良的铜绿假单胞菌肽蛋白肽,以防止皮林的低聚。 该方法包括将组合物给予感染了假单胞菌的人患有这种感染的风险。
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