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公开(公告)号:US20120244534A1
公开(公告)日:2012-09-27
申请号:US13397416
申请日:2012-02-15
申请人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
发明人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
CPC分类号: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
摘要: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
摘要翻译: 本发明涉及用于进行多级扩增反应的系统,方法和装置,特别是在流体封闭的条件下。 在一个方面,本发明的方法在流体封闭的反应体系中进行,其允许分离第一(或先前)反应混合物的一部分,并将其用作第二(或后续)反应混合物中的样品或样品 ,从而基本上避免了如果两个反应混合物简单地组合在一起,第一反应组分可能在第二反应中可能具有干扰效应。 在这方面,本发明的系统,方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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公开(公告)号:US08728765B2
公开(公告)日:2014-05-20
申请号:US13397416
申请日:2012-02-15
申请人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wong , Kendra Lara Paul , Reuel Van Atta
发明人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wong , Kendra Lara Paul , Reuel Van Atta
IPC分类号: C12P19/34
CPC分类号: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
摘要: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
摘要翻译: 本发明涉及用于进行多级扩增反应的系统,方法和装置,特别是在流体封闭的条件下。 在一个方面,本发明的方法在流体封闭的反应体系中进行,其允许分离第一(或先前)反应混合物的一部分,并将其用作第二(或后续)反应混合物中的样品或样品 ,从而基本上避免了如果两个反应混合物简单地组合在一起,第一反应组分可能在第二反应中可能具有干扰效应。 在这方面,本发明的系统,方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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公开(公告)号:US20060177844A1
公开(公告)日:2006-08-10
申请号:US11262523
申请日:2005-10-27
申请人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Wang , Wendy Wong , Kendra Paul , Reuel Van Atta
发明人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Wang , Wendy Wong , Kendra Paul , Reuel Van Atta
CPC分类号: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
摘要: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
摘要翻译: 本发明涉及用于进行多级扩增反应的系统,方法和装置,特别是在流体封闭的条件下。 在一个方面,本发明的方法在流体封闭的反应体系中进行,其允许分离第一(或先前)反应混合物的一部分,并将其用作第二(或后续)反应混合物中的样品或样品 ,从而基本上避免了如果两个反应混合物简单地组合在一起,第一反应组分可能在第二反应中可能具有干扰效应。 在这方面,本发明的系统,方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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公开(公告)号:US20080057572A1
公开(公告)日:2008-03-06
申请号:US11977697
申请日:2007-10-24
申请人: Kurt Petersen , Michael Taylor , Farzad Pourahmadi , William McMillan , Ronald Chang , Stanley Sakai , Jesus Ching , Douglas Dority , Phillip Belgrader , M. Northrup
发明人: Kurt Petersen , Michael Taylor , Farzad Pourahmadi , William McMillan , Ronald Chang , Stanley Sakai , Jesus Ching , Douglas Dority , Phillip Belgrader , M. Northrup
IPC分类号: C12M3/00
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体,裂解细胞或病毒以从其中分离分析物(例如核酸),以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断,生命科学研究,环境或法医应用,用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号:US20050042137A1
公开(公告)日:2005-02-24
申请号:US10955811
申请日:2004-09-29
CPC分类号: B01L7/52 , B01L3/502 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0858 , B01L2300/087 , B01L2300/123 , B01L2300/14 , B01L2300/168 , B01L2300/18 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12Q1/6844 , G01N33/5302 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要: A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.
摘要翻译: 用于进行化学反应的药筒包括具有形成在其中的至少一个流路的主体。 药筒还包括从身体延伸的反应容器,用于保持用于化学反应和光学检测的反应混合物。 容器包括限定反应室侧壁的刚性框架。 该框架包括连接到该腔室的流动路径的至少一个通道。 容器还包括附接到刚性框架的相对侧的柔性膜或片,以形成腔室的相对的主壁。 此外,至少两个侧壁是光学透射的并且与每个侧壁成角度地偏移以允许对反应室中的分析物的实时光学检测。
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公开(公告)号:US20060275178A1
公开(公告)日:2006-12-07
申请号:US11146304
申请日:2005-06-06
申请人: Ronald Chang , Douglas Dority , Steven Montgomery
发明人: Ronald Chang , Douglas Dority , Steven Montgomery
IPC分类号: B01L9/00
CPC分类号: B01L3/527 , B01J19/0046 , B01J2219/00468 , B01J2219/005 , B01J2219/00657 , B01L3/0289 , B01L2200/0647 , B01L2200/16 , G01N2035/00574 , Y10T436/25
摘要: Embodiments of the invention provide an efficient and effective technique for storing and dispensing reagent beads. In one embodiment, an apparatus is provided for dispensing reagent beads contained in a bead storage device which includes a bead carrier having a plurality of wells; a plurality of reagent beads disposed in the wells; and a cover tape releasably attached to the bead carrier to cover the wells and retain the reagent beads in the wells. The apparatus comprises a channel in which to place the bead storage device with the bead carrier facing a support wall of the channel and the cover tape facing a stripping wall of the channel. The stripping wall includes a stripping gap disposed between a stripping edge and an opposite edge, and a dispense opening provided adjacent the opposite edge on a side of the stripping wall opposite from the stripping edge. The cover tape is insertable through the stripping gap to be pulled against the stripping edge to peel the cover tape from the bead carrier to move the wells of the bead carrier inside the channel toward the dispense opening and expose the wells individually to dispense the reagent beads.
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公开(公告)号:US08709363B2
公开(公告)日:2014-04-29
申请号:US13436475
申请日:2012-03-30
CPC分类号: B01L7/52 , B01L3/502 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0858 , B01L2300/087 , B01L2300/123 , B01L2300/14 , B01L2300/168 , B01L2300/18 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12Q1/6844 , G01N33/5302 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要: A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.
摘要翻译: 用于进行化学反应的药筒包括具有形成在其中的至少一个流路的主体。 药筒还包括从身体延伸的反应容器,用于保持用于化学反应和光学检测的反应混合物。 容器包括限定反应室侧壁的刚性框架。 该框架包括连接到该腔室的流动路径的至少一个通道。 容器还包括附接到刚性框架的相对侧的柔性膜或片,以形成腔室的相对的主壁。 此外,至少两个侧壁是光学透射的并且与每个侧壁成角度地偏移以允许对反应室中的分析物的实时光学检测。
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