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    Gene transfer in intact mammals
    4.
    发明授权
    Gene transfer in intact mammals 失效
    完整哺乳动物的基因转移

    公开(公告)号：US4497796A

    公开(公告)日：1985-02-05

    申请号：US447858

    申请日：1982-12-08

    申请人： Winston A. Salser ,  Martin J. Cline ,  Howard D. Stang

    发明人： Winston A. Salser ,  Martin J. Cline ,  Howard D. Stang

    IPC分类号： C12N15/00 ,  A61K35/12

    CPC分类号： C12N15/00

    摘要： Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Particularly, mammalian cells were modified with genes providing for overproduction of a particular enzyme. The modified cells were reintroduced in the host under conditions providing for selective advantage of the modified cells.

    摘要翻译： 提供方法和组合物用于在宿主中表达外源性遗传物质的基因转移到完整的哺乳动物。 再生的，通常高度增殖或受到诱导增殖的哺乳动物宿主细胞在体外用能够在宿主细胞中复制和表达的DNA进行转化或修饰，其中DNA被并入细胞中。 发现修饰的细胞在宿主中通过引入的DNA的表达再生。 特别地，用提供特定酶的过度产生的基因修饰哺乳动物细胞。 在提供修饰细胞的选择性优势的条件下，将修饰的细胞重新引入宿主。

    Gene transfer in intact mammals
    8.
    发明授权
    Gene transfer in intact mammals 失效

    公开(公告)号：US4396601A

    公开(公告)日：1983-08-02

    申请号：US134234

    申请日：1980-03-26

    申请人： Winston A. Salser ,  Martin J. Cline ,  Howard D. Stang

    发明人： Winston A. Salser ,  Martin J. Cline ,  Howard D. Stang

    IPC分类号： A01K67/027 ,  A61K37/48 ,  A61K35/14

    CPC分类号： A01K67/0275 ,  A01K2217/05

    摘要： Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Particularly, mammalian cells were modified with genes providing for overproduction of a particular enzyme. The modified cells were reintroduced in the host under conditions providing for selective advantage of the modified cells.