公开(公告)号：US08663967B2

公开(公告)日：2014-03-04

申请号：US13214398

申请日：2011-08-22

申请人： Yanshan Huang ,  Jiwan Qiu ,  Xiaoyu Fu ,  Min Fan ,  Yujiao Wang ,  Yefei Wang

发明人： Yanshan Huang ,  Jiwan Qiu ,  Xiaoyu Fu ,  Min Fan ,  Yujiao Wang ,  Yefei Wang

IPC分类号： C12N11/02 ,  C12N11/08 ,  C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/78 ,  A61K38/00 ,  C12Y305/03006

摘要： The present invention relates to an arginine deiminase mutant with partial lysine-deficient and preparation and application thereof. The arginine deiminase mutant of the present invention has enzymatic activity of degrading arginine into citruline; compared with the arginine deiminase with the amino acid sequence of SEQ ID NO: 1, the amino acid sequence comprises one or more of K9N, T, K59Q, K66R, A, K93E, A, Q, K111R, A, K119Q, L, M, K121Q, I, K122E, L, K126E, S, R, K178I, E, D, K196I, R, K209G, T, D, K243E, V, R, K249D, Q, K263N, Q, K279Y, T, K293R, H, E, K325V, I, K380T, R, E, and K406E, D, S substitutions. Compared with PEG modified natural derived arginine deiminase, the PEG modified arginine deiminase mutant of the present invention retain better bioactivity; and because the quantity of lysine in arginine deiminase is reduced, the PEG modified products are more uniform and can be applied to clinical treatment of hepatoma, melanoma and the like.

摘要翻译： 本发明涉及具有部分赖氨酸缺陷型及其制备及应用的精氨酸脱亚胺酶突变体。 本发明的精氨酸脱亚胺酶突变体具有将精氨酸降解成柠檬酸的酶活性; 与具有SEQ ID NO：1的氨基酸序列的精氨酸脱亚氨酶相比，氨基酸序列包含K9N，T，K59Q，K66R，A，K93E，A，Q，K111R，A，K119Q，L， M，K121Q，I，K122E，L，K126E，S，R，K178I，E，D，K196I，R，K209G，T，D，K243E，V，R，K249D，Q，K263N，Q，K279Y， K293R，H，E，K325V，I，K380T，R，E和K406E，D，S取代。 与PEG改性天然衍生的精氨酸脱亚胺酶相比，本发明的PEG修饰的精氨酸脱亚氨酶突变体保持较好的生物活性; 并且由于精氨酸去酰亚胺酶中赖氨酸的量减少，PEG改性产物更均匀，可用于肝癌，黑素瘤等的临床治疗。

公开(公告)号：USRE44622E1

公开(公告)日：2013-12-03

申请号：US13645489

申请日：2012-10-04

申请人： Xiaoyu Fu ,  Jun Ma ,  Nabil Yousef

发明人： Xiaoyu Fu ,  Jun Ma ,  Nabil Yousef

IPC分类号： H04K1/10

CPC分类号： H04L27/2676 ,  H04L27/2659

摘要： A method of estimating coarse frequency offset of received symbols based on a received frequency domain sample at a kth sub-carrier of a 53rd Orthogonal Frequency Division Multiplexing (OFDM) data symbol in a jth time slot (TS) of a receiver in a China Multimedia Mobile Broadcasting (CMMB) mobile television communication network includes dividing a received sample, Ykj, into two sets of noise only tones and data plus noise tones Dkj, obtaining a received sample only if there is a coarse frequency offset mismatch between a transmitter and the receiver, dividing a summation of a power of the data plus noise tones by a summation of a power of the noise only tones to obtain Λkj, and estimating an integer coarse frequency offset estimate, Δ{circumflex over (f)}Ij, of the received symbols when the Λkj is a maximum.

摘要翻译： 一种基于中国多媒体接收机的第j个时隙（TS）中第53个正交频分复用（OFDM）数据符号的第k个子载波的接收频域样本估计接收符号的粗略偏移的方法 移动广播（CMMB）移动电视通信网络包括将接收到的采样Ykj划分为两组仅噪声音调和数据加噪声音调Dkj，仅在发射机和接收机之间存在粗略的频率偏移失配时才获得接收的采样 将所述数据的功率和噪声音调的加和除以所述仅噪声音调的功率的和，以获得Lambdakj，并且估计所接收到的所述接收到的噪声音调的整数粗略频率偏移估计值Delta（f（f）} Ij） 当Lambdakj是最大值时的符号。

公开(公告)号：US07941117B2

公开(公告)日：2011-05-10

申请号：US12039806

申请日：2008-02-29

申请人： Jun Ma ,  Ahmed Eltawil ,  Xiaoyu Fu

发明人： Jun Ma ,  Ahmed Eltawil ,  Xiaoyu Fu

IPC分类号： H04B1/10

CPC分类号： H04B1/30 ,  H04L27/3863 ,  H04L27/3872

摘要： A method of processing an in-phase signal component and a quadrature signal component of a low intermediate frequency (LIF) signal includes estimating and correcting an amplitude imbalance between a digitized in-phase signal component and a digitized quadrature signal component at a first point in time, and estimating and correcting a phase imbalance between the digitized in-phase signal component and the digitized quadrature signal component at a second point in time in response to the correcting process. The digitized in-phase signal component corresponds to the in-phase signal component at the first point in time and the digitized quadrature signal component corresponds to the quadrature signal component at the first point in time. The second point in the time is subsequent to the first point in time.

摘要翻译： 处理低中频（LIF）信号的同相信号分量和正交信号分量的方法包括：估计和校正数字化的同相信号分量与数字化正交信号分量之间的振幅不平衡 时间，并且响应于校正处理，在第二时间点估计和校正数字化同相信号分量和数字化正交信号分量之间的相位不平衡。 数字化的同相信号分量对应于第一时间点的同相信号分量，数字化的正交信号分量对应于第一时间点的正交信号分量。 第二点在第一时间点之后。

公开(公告)号：US20090221255A1

公开(公告)日：2009-09-03

申请号：US12039806

申请日：2008-02-29

申请人： Jun Ma ,  Ahmed Eltawil ,  Xiaoyu Fu

发明人： Jun Ma ,  Ahmed Eltawil ,  Xiaoyu Fu

IPC分类号： H04B1/10 ,  H03H11/16 ,  H03L5/00

CPC分类号： H04B1/30 ,  H04L27/3863 ,  H04L27/3872

摘要： A method of processing an in-phase signal component and a quadrature signal component of a low intermediate frequency (LIF) signal includes estimating and correcting an amplitude imbalance between a digitized in-phase signal component and a digitized quadrature signal component at a first point in time, and estimating and correcting a phase imbalance between the digitized in-phase signal component and the digitized quadrature signal component at a second point in time in response to the correcting process. The digitized in-phase signal component corresponds to the in-phase signal component at the first point in time and the digitized quadrature signal component corresponds to the quadrature signal component at the first point in time. The second point in the time is subsequent to the first point in time.

摘要翻译： 处理低中频（LIF）信号的同相信号分量和正交信号分量的方法包括：估计和校正数字化的同相信号分量与数字化正交信号分量之间的振幅不平衡 时间，并且响应于校正处理，在第二时间点估计和校正数字化同相信号分量和数字化正交信号分量之间的相位不平衡。 数字化的同相信号分量对应于第一时间点的同相信号分量，数字化的正交信号分量对应于第一时间点的正交信号分量。 第二点在第一时间点之后。

公开(公告)号：US07944983B2

公开(公告)日：2011-05-17

申请号：US12039799

申请日：2008-02-29

申请人： Xiaoyu Fu ,  Jun Ma ,  Nabil Yousef

发明人： Xiaoyu Fu ,  Jun Ma ,  Nabil Yousef

IPC分类号： H04K1/10

CPC分类号： H04L27/2676 ,  H04L27/2659

摘要： A method of estimating coarse frequency offset of received symbols based on a received frequency domain sample at a kth sub-carrier of a 53rd Orthogonal Frequency Division Multiplexing (OFDM) data symbol in a jth time slot (TS) of a receiver in a China Multimedia Mobile Broadcasting (CMMB) mobile television network includes dividing a received sample, Ykj, into two sets of noise only tones and data plus noise tones Dkj, obtaining a received sample only if there is a coarse frequency offset mismatch between a transmitter and the receiver, dividing a summation of a power of the data plus noise tones by a summation of a power of the noise only tones to obtain Λkj, and estimating an integer coarse frequency offset estimate, Δ{circumflex over (f)}Ij, of the received symbols when the Λkj is a maximum.

摘要翻译： 一种基于中国多媒体接收机的第j个时隙（TS）中第53个正交频分复用（OFDM）数据符号的第k个子载波的接收频域样本估计接收符号的粗略偏移的方法 移动广播（CMMB）移动电视网络包括将接收到的采样Ykj划分为两组仅噪声的音调和数据加噪声音调Dkj，仅在发射机和接收机之间存在粗略的频率偏移不匹配时才获得接收的采样， 通过仅噪声音调的功率的总和除以数据的加权和噪声音调的和，以获得Λkj，并且估计所接收到的噪声的整数粗频率偏移估计，并且（D（f）} Ij） ∧kj为最大值时的符号。

公开(公告)号：US20130052179A1

公开(公告)日：2013-02-28

申请号：US13214398

申请日：2011-08-22

申请人： Yanshan HUANG ,  Jiwan Qiu ,  Xiaoyu Fu ,  Min Fan ,  Yujiao Wang ,  Yefei Wang

发明人： Yanshan HUANG ,  Jiwan Qiu ,  Xiaoyu Fu ,  Min Fan ,  Yujiao Wang ,  Yefei Wang

IPC分类号： A61K38/46 ,  C12N11/02 ,  C12N11/10 ,  C12N11/08 ,  C12N15/55 ,  A61P35/00 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  A61P31/12 ,  A61P25/28 ,  C12N9/78 ,  C12N15/63

CPC分类号： C12N9/78 ,  A61K38/00 ,  C12Y305/03006

摘要： The present invention relates to an arginine deiminase mutant with partial lysine-deficient and preparation and application thereof. The arginine deiminase mutant of the present invention has enzymatic activity of degrading arginine into citruline; compared with the arginine deiminase with the amino acid sequence of SEQ ID NO: 1, the amino acid sequence comprises one or more of K9N, T, K59Q, K66R, A, K93E, A, Q, K111R, A, K119Q, L, M, K121Q, I, K122E, L, K126E, S, R, K178I, E, D, K196I, R, K209G, T, D, K243E, V, R, K249D, Q, K263N, Q, K279Y, T, K293R, H, E, K325V, I, K380T, R, E, and K406E, D, S substitutions. Compared with PEG modified natural derived arginine deiminase, the PEG modified arginine deiminase mutant of the present invention retain better bioactivity; and because the quantity of lysine in arginine deiminase is reduced, the PEG modified products are more uniform and can be applied to clinical treatment of hepatoma, melanoma and the like.

摘要翻译： 本发明涉及具有部分赖氨酸缺陷型及其制备及应用的精氨酸脱亚胺酶突变体。 本发明的精氨酸脱亚胺酶突变体具有将精氨酸降解成柠檬酸的酶活性; 与具有SEQ ID NO：1的氨基酸序列的精氨酸脱亚氨酶相比，氨基酸序列包含K9N，T，K59Q，K66R，A，K93E，A，Q，K111R，A，K119Q，L， M，K121Q，I，K122E，L，K126E，S，R，K178I，E，D，K196I，R，K209G，T，D，K243E，V，R，K249D，Q，K263N，Q，K279Y， K293R，H，E，K325V，I，K380T，R，E和K406E，D，S取代。 与PEG改性天然衍生的精氨酸脱亚胺酶相比，本发明的PEG修饰的精氨酸脱亚氨酶突变体保持较好的生物活性; 并且由于精氨酸去酰亚胺酶中赖氨酸的量减少，PEG改性产物更均匀，可用于肝癌，黑素瘤等的临床治疗。

公开(公告)号：US08169546B2

公开(公告)日：2012-05-01

申请号：US12033323

申请日：2008-02-19

申请人： Xiaoyu Fu ,  Jun Ma ,  Waleed M. Younis ,  Nabil Yousef ,  Janakan Sivasubramaniam ,  Edward Youssoufian

发明人： Xiaoyu Fu ,  Jun Ma ,  Waleed M. Younis ,  Nabil Yousef ,  Janakan Sivasubramaniam ,  Edward Youssoufian

IPC分类号： H04N5/52 ,  H04N3/27 ,  H04N5/00 ,  H04N5/44 ,  H04B1/40 ,  H04B17/02 ,  H04B1/06 ,  H04B7/00 ,  H04B1/26 ,  H04B1/00 ,  H04L27/06 ,  H04L17/08

CPC分类号： H04N5/52 ,  H03G3/001 ,  H03G3/3068 ,  H04N5/4446

摘要： A technique for providing stable tracking performance to an AGC loop circuit comprises amplifying a wideband radio frequency signal; detecting signals and blockers adjacent to the radio frequency signal; lowering a gain of the radio frequency signal; mixing a local oscillator signal with the radio frequency signal; shifting a frequency of the radio frequency signal from a radio frequency to an intermediate frequency; continuously varying a gain of the intermediate frequency signal; converting the intermediate frequency signal into a digital output signal; comparing the digital output signal with predefined thresholds comprising an upper threshold and a lower threshold; switching a post mixer amplifier (PMA) to a high gain state when an input of a variable gain amplifier (VGA) is greater than the upper threshold, and switching the PMA to a low gain state when an input of the VGA is lower than the lower threshold.

摘要翻译： 一种用于向AGC环路电路提供稳定跟踪性能的技术包括放大宽带射频信号; 检测与射频信号相邻的信号和阻塞; 降低射频信号的增益; 将本地振荡器信号与射频信号混合; 将射频信号的频率从射频转换到中频; 连续地改变中频信号的增益; 将所述中频信号转换为数字输出信号; 将数字输出信号与包括上阈值和下阈值的预定义阈值进行比较; 当可变增益放大器（VGA）的输入大于上阈值时，将后混频器放大器（PMA）切换到高增益状态，并且当VGA的输入低于该阈值时，将PMA切换到低增益状态 较低的门槛。

公开(公告)号：US20090220015A1

公开(公告)日：2009-09-03

申请号：US12039799

申请日：2008-02-29

申请人： Xiaoyu Fu ,  Jun Ma ,  Nabil Yousef

发明人： Xiaoyu Fu ,  Jun Ma ,  Nabil Yousef

IPC分类号： H04K1/10 ,  H04H60/04

CPC分类号： H04L27/2676 ,  H04L27/2659

摘要： A method of estimating coarse frequency offset of received symbols based on a received frequency domain sample at a kth sub-carrier of a 53rd Orthogonal Frequency Division Multiplexing (OFDM) data symbol in a jth time slot (TS) of a receiver in a China Multimedia Mobile Broadcasting (CMMB) mobile television network includes dividing a received sample, Ykj, into two sets of noise only tones and data plus noise tones Dkj, obtaining a received sample only if there is a coarse frequency offset mismatch between a transmitter and the receiver, dividing a summation of a power of the data plus noise tones by a summation of a power of the noise only tones to obtain Λkj, and estimating an integer coarse frequency offset estimate, Δ{circumflex over (f)}Ij, of the received symbols when the Λkj is a maximum.

摘要翻译： 一种基于中国多媒体接收机的第j个时隙（TS）中第53个正交频分复用（OFDM）数据符号的第k个子载波的接收频域采样来估计接收符号的粗频偏移的方法 移动广播（CMMB）移动电视网络包括将接收到的采样Ykj划分为两组仅噪声的音调和数据加噪声音调Dkj，仅在发射机和接收机之间存在粗略的频率偏移不匹配时才获得接收的采样， 通过仅噪声音调的功率的总和除以数据的加权和噪声音调的和，以获得Lambdakj，并且估计接收符号的整数粗略频率偏移估计值Delta（（f）} Ij） 当Lambdakj是最大值。

公开(公告)号：US20090207311A1

公开(公告)日：2009-08-20

申请号：US12033323

申请日：2008-02-19

申请人： Xiaoyu Fu ,  Jun Ma ,  Waleed M. Younis ,  Nabil Yousef ,  Janakan Sivasubramaniam ,  Edward Youssoufian

发明人： Xiaoyu Fu ,  Jun Ma ,  Waleed M. Younis ,  Nabil Yousef ,  Janakan Sivasubramaniam ,  Edward Youssoufian

IPC分类号： H04N5/52 ,  H04B1/06

CPC分类号： H04N5/52 ,  H03G3/001 ,  H03G3/3068 ,  H04N5/4446

摘要： A technique for providing stable tracking performance to an AGC loop circuit comprises amplifying a wideband radio frequency signal; detecting signals and blockers adjacent to the radio frequency signal; lowering a gain of the radio frequency signal; mixing a local oscillator signal with the radio frequency signal; shifting a frequency of the radio frequency signal from a radio frequency to an intermediate frequency; continuously varying a gain of the intermediate frequency signal; converting the intermediate frequency signal into a digital output signal; comparing the digital output signal with predefined thresholds comprising an upper threshold and a lower threshold; switching a post mixer amplifier (PMA) to a high gain state when an input of a variable gain amplifier (VGA) is greater than the upper threshold, and switching the PMA to a low gain state when an input of the VGA is lower than the lower threshold.

摘要翻译： 一种用于向AGC环路电路提供稳定跟踪性能的技术包括放大宽带射频信号; 检测与射频信号相邻的信号和阻塞; 降低射频信号的增益; 将本地振荡器信号与射频信号混合; 将射频信号的频率从射频转换到中频; 连续地改变中频信号的增益; 将所述中频信号转换为数字输出信号; 将数字输出信号与包括上阈值和下阈值的预定阈值进行比较; 当可变增益放大器（VGA）的输入大于上阈值时，将后混频器放大器（PMA）切换到高增益状态，并且当VGA的输入低于该阈值时，将PMA切换到低增益状态 较低的门槛。