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公开(公告)号：US07005518B2
公开(公告)日：2006-02-28
申请号：US10693300
申请日：2003-10-23
Applicant: Xinzhan Peng , Daniel R. Draney , Jiyan Chen
Inventor： Xinzhan Peng , Daniel R. Draney , Jiyan Chen
IPC: C07B47/00 , C07F5/10 , A61B10/00 , C07D487/22 , C09B47/04
CPC classification number: C09B47/32 , C09B69/008
Abstract: Fluorescent dyes are disclosed which are useful as reporter groups for labeling biomolecules. The silicon phthalocyanine dyes disclosed are preferably water soluble, isomericly pure, possess high quantum yield, and are useful in bioassays.
Abstract translation: 公开了可用作标记生物分子的报告基团的荧光染料。 所公开的硅酞菁染料优选是水溶性的，异构纯的，具有高量子产率，并且可用于生物测定。
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公开(公告)号：US20110014612A1
公开(公告)日：2011-01-20
申请号：US12748359
申请日：2010-03-26
Applicant: STEPHEN P. HENDRICKS , MICHAEL PHELAN , MARIAN PERIS , CHENG-YAO CHEN , DANIEL MAZUR , XINZHAN PENG , AMY CASTILLO
Inventor： STEPHEN P. HENDRICKS , MICHAEL PHELAN , MARIAN PERIS , CHENG-YAO CHEN , DANIEL MAZUR , XINZHAN PENG , AMY CASTILLO
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
Abstract translation: 本文公开了显示改变的聚合酶活性的修饰聚合酶组合物，其可用于多种生物应用。 本文还公开了制备和使用这些组合物的方法。 在一些实施方案中，组合物显示改变的性质，其可以增强其在各种生物应用中的效用。 这种改变的性质可以包括例如改变的核苷酸结合亲和力，改变的核苷酸掺入动力学，改变的光稳定性和/或改变的纳米颗粒耐受性，以及本文公开的一系列其它性质。
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公开(公告)号：US20120322057A1
公开(公告)日：2012-12-20
申请号：US13540935
申请日：2012-07-03
Applicant: Stephen P. HENDRICKS , Michael PHELAN , Marian PERIS , Cheng-Yao CHEN , Daniel MAZUR , Xinzhan PENG , Amy CASTILLO
Inventor： Stephen P. HENDRICKS , Michael PHELAN , Marian PERIS , Cheng-Yao CHEN , Daniel MAZUR , Xinzhan PENG , Amy CASTILLO
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
Abstract translation: 本文公开了显示改变的聚合酶活性的修饰聚合酶组合物，其可用于多种生物应用。 本文还公开了制备和使用这些组合物的方法。 在一些实施方案中，组合物显示改变的性质，其可以增强其在各种生物应用中的效用。 这种改变的性质可以包括例如改变的核苷酸结合亲和力，改变的核苷酸掺入动力学，改变的光稳定性和/或改变的纳米颗粒耐受性，以及本文公开的一系列其它性质。
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公开(公告)号：US20070042398A1
公开(公告)日：2007-02-22
申请号：US11423675
申请日：2006-06-12
Applicant: Xinzhan Peng , Xinshe Xu , Daniel Draney , Garrick Little , Jiyan Chen , William Volcheck , Charles Prescott
Inventor： Xinzhan Peng , Xinshe Xu , Daniel Draney , Garrick Little , Jiyan Chen , William Volcheck , Charles Prescott
IPC: C12Q1/68 , G01N33/53 , C07D403/14 , C07D403/02
CPC classification number: G01N33/542 , C09B23/0066 , C09B23/083 , G01N33/532
Abstract: The present invention provides for cyanine dyes as near IR quenchers. The cyanine dyes have absorption wavelengths in the near-infrared region of about 650-900 nm and are essentially non-fluorescent. The dyes of the invention have at least one linking group. The present invention also provides substantially non-fluorescent, NIR probes. Biological assays based on these novel, substantially non-fluorescent, NIR probes are also provided.
Abstract translation: 本发明提供花青染料作为近红外猝灭剂。 花青染料在约650-900nm的近红外区域具有吸收波长，并且基本上是非荧光的。 本发明的染料具有至少一个连接基团。 本发明还提供基本上非荧光的NIR探针。 还提供了基于这些新颖的，基本上非荧光的NIR探针的生物测定。
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公开(公告)号：US08603792B2
公开(公告)日：2013-12-10
申请号：US12748355
申请日：2010-03-26
Applicant: Theo Nikiforov , Daniel Mazur , Xinzhan Peng , Tommie Lloyd Lincecum, Jr. , Yuri Belosludtsev , Howard Reese , Dmitriy Gremyachinskiy , Roman Rozhkov , John M. Mauro , Joseph Beechem , Eric Tulsky , Imad Naasani , Kari Haley , Joseph A. Treadway
Inventor： Theo Nikiforov , Daniel Mazur , Xinzhan Peng , Tommie Lloyd Lincecum, Jr. , Yuri Belosludtsev , Howard Reese , Dmitriy Gremyachinskiy , Roman Rozhkov , John M. Mauro , Joseph Beechem , Eric Tulsky , Imad Naasani , Kari Haley , Joseph A. Treadway
IPC: C12N9/12
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
Abstract translation: 本文公开了包含连接到具有生物活性并可用于多种生物应用的标签的生物分子的缀合物。 例如，本文提供了聚合酶 - 纳米颗粒缀合物，其包括与纳米颗粒连接的聚合酶，其中缀合物具有聚合酶活性。 这样的缀合物可以表现出减少的聚集和改进的沉淀物，其中平均生物分子：纳米颗粒比接近或等于1:1。 本文还公开了制备这种缀合物的改进方法，以及在生物应用中使用这种缀合物的方法和系统，例如核苷酸掺入，引物延伸和单分子测序。
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Patent Agency Ranking
6.发明申请METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION 有权使用能量转移检测的单分子序列的方法和装置公开(公告)号：US20120329042A1
公开(公告)日：2012-12-27
申请号：US13562159
申请日：2012-07-30
Applicant: Joseph BEECHEM , Theo NIKIFOROV , Vi-En CHOONG , Xinzhan PENG , Guobin LUO , Cheng-Yao CHEN , Michael PREVITE
Inventor： Joseph BEECHEM , Theo NIKIFOROV , Vi-En CHOONG , Xinzhan PENG , Guobin LUO , Cheng-Yao CHEN , Michael PREVITE
IPC: G01N21/64
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.
Abstract translation: 本文提供了用于核苷酸掺入反应的系统和方法。 该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。 当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。 当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。 检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。
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7.发明授权Methods and apparatus for single molecule sequencing using energy transfer detection 有权使用能量转移检测的单分子测序的方法和装置公开(公告)号：US08999674B2
公开(公告)日：2015-04-07
申请号：US13562159
申请日：2012-07-30
Applicant: Joseph Beechem , Theo Nikiforov , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
Inventor： Joseph Beechem , Theo Nikiforov , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.
Abstract translation: 本文提供了用于核苷酸掺入反应的系统和方法。 该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。 当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。 当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。 检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。
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公开(公告)号：US08227621B2
公开(公告)日：2012-07-24
申请号：US11423675
申请日：2006-06-12
Applicant: Xinzhan Peng , Xinshe Xu , Daniel R. Draney , Garrick M. Little , Jiyan Chen , William M. Volcheck , Charles Prescott
Inventor： Xinzhan Peng , Xinshe Xu , Daniel R. Draney , Garrick M. Little , Jiyan Chen , William M. Volcheck , Charles Prescott
IPC: C07D209/56 , A61B5/00
CPC classification number: G01N33/542 , C09B23/0066 , C09B23/083 , G01N33/532
Abstract: The present invention provides for cyanine dyes as near IR quenchers. The cyanine dyes have absorption wavelengths in the near-infrared region of about 650-900 nm and are essentially non-fluorescent. The dyes of the invention have at least one linking group. The present invention also provides substantially non-fluorescent, NIR probes. Biological assays based on these novel, substantially non-fluorescent, NIR probes are also provided.
Abstract translation: 本发明提供花青染料作为近红外猝灭剂。 花青染料在约650-900nm的近红外区域具有吸收波长，并且基本上是非荧光的。 本发明的染料具有至少一个连接基团。 本发明还提供基本上非荧光的NIR探针。 还提供了基于这些新颖的，基本上非荧光的NIR探针的生物测定。
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公开(公告)号：US20110003343A1
公开(公告)日：2011-01-06
申请号：US12748355
申请日：2010-03-26
Applicant: Theo Nikiforov , Daniel Mazur , Xinzhan Peng , Tommie Lloyd Lincecum, JR. , Yuri Belosludtsev , Howard Reese , Dmitriy Gremyachinskiy , Roman Rozhkov , John M. Mauro , Joseph Beechem , Eric Tulsky , Imad Naasani , Kari Haley , Joseph A. Treadway
Inventor： Theo Nikiforov , Daniel Mazur , Xinzhan Peng , Tommie Lloyd Lincecum, JR. , Yuri Belosludtsev , Howard Reese , Dmitriy Gremyachinskiy , Roman Rozhkov , John M. Mauro , Joseph Beechem , Eric Tulsky , Imad Naasani , Kari Haley , Joseph A. Treadway
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
Abstract translation: 本文公开了包含连接到具有生物活性并可用于多种生物应用的标签的生物分子的缀合物。 例如，本文提供了聚合酶 - 纳米颗粒缀合物，其包括与纳米颗粒连接的聚合酶，其中缀合物具有聚合酶活性。 这样的缀合物可以表现出减少的聚集和改进的沉淀物，其中平均生物分子：纳米颗粒比接近或等于1:1。 本文还公开了制备这种缀合物的改进方法，以及在生物应用中使用这种缀合物的方法和系统，例如核苷酸掺入，引物延伸和单分子测序。
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10.发明申请METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION 审中-公开使用能量转移检测的单分子序列的方法和装置公开(公告)号：US20100255487A1
公开(公告)日：2010-10-07
申请号：US12748168
申请日：2010-03-26
Applicant: JOSEPH BEECHEM , THEO NIKIFOROV , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
Inventor： JOSEPH BEECHEM , THEO NIKIFOROV , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.
Abstract translation: 本文提供了用于核苷酸掺入反应的系统和方法。 该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。 当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。 当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。 检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。
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