公开(公告)号：US20210010072A1

公开(公告)日：2021-01-14

申请号：US16766125

申请日：2018-11-22

申请人： Koei SUZUKI ,  Hirotaka UNNO ,  Manabu SEO ,  Satoshi IZUMI ,  Yusuke OSAKI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO

发明人： Koei SUZUKI ,  Hirotaka UNNO ,  Manabu SEO ,  Satoshi IZUMI ,  Yusuke OSAKI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO

IPC分类号： C12Q1/6851 ,  C12Q1/686

摘要： Provided is a device including: a plurality of wells; a reagent composition located in each of the wells and containing an amplifiable reagent in a specific copy number; and two or more groups into which the wells are divided to have the amplifiable reagent located in the same specific copy number but to be varied in composition of the reagent composition except for the specific copy number. Preferably, composition except for the specific copy number of the amplifiable reagent includes at least any one of primer and amplifying reagent. More preferably, the device includes two or more groups varied in the specific copy number. Yet more preferably, the groups of wells include at least a negative control group in which the specific copy number of the amplifiable reagent is 0, and a group in which the specific copy number of the amplifiable reagent is close to the limit of detection.

公开(公告)号：US11040349B2

公开(公告)日：2021-06-22

申请号：US16196790

申请日：2018-11-20

申请人： Yudai Kawashima ,  Satoshi Izumi ,  Manabu Seo

发明人： Yudai Kawashima ,  Satoshi Izumi ,  Manabu Seo

IPC分类号： B01L3/00 ,  B01L3/02 ,  G01N15/14 ,  C12Q1/686 ,  C12Q1/6844 ,  G01N15/10

摘要： Provided is a device including: at least one well in which an amplifiable reagent is contained in a specific copy number of less than 100; and at least one well in which the amplifiable reagent is contained in a specific copy number of 100 or greater, wherein for the at least one well in which the specific copy number of the amplifiable reagent is less than 100, a formula: CV

公开(公告)号：US20190151843A1

公开(公告)日：2019-05-23

申请号：US16196790

申请日：2018-11-20

申请人： Yudai Kawashima ,  Satoshi Izumi ,  Manabu Seo

发明人： Yudai Kawashima ,  Satoshi Izumi ,  Manabu Seo

IPC分类号： B01L3/00 ,  C12Q1/6844

摘要： Provided is a device including: at least one well in which an amplifiable reagent is contained in a specific copy number of less than 100; and at least one well in which the amplifiable reagent is contained in a specific copy number of 100 or greater, wherein for the at least one well in which the specific copy number of the amplifiable reagent is less than 100, a formula: CV

公开(公告)号：US20190136304A1

公开(公告)日：2019-05-09

申请号：US16161203

申请日：2018-10-16

申请人： Manabu SEO ,  Satoshi IZUMI ,  Yudai KAWASHIMA

发明人： Manabu SEO ,  Satoshi IZUMI ,  Yudai KAWASHIMA

IPC分类号： C12Q1/6844

摘要： Provided is a detection accuracy identifying method for identifying a detection accuracy for detecting a testing target nucleic acid, the method including identifying a detection accuracy ability of the testing target nucleic acid, using a standard substance containing a known number of nucleic acid molecule and based on a probability at which the known number of nucleic acid molecule is detected.

公开(公告)号：US20230323295A1

公开(公告)日：2023-10-12

申请号：US18176959

申请日：2023-03-01

申请人： Masayuki YUMOTO ,  Yudai KAWASHIMA ,  Hikaru WATANABE ,  Toshihiko HOSOYA ,  Minoru KO

发明人： Masayuki YUMOTO ,  Yudai KAWASHIMA ,  Hikaru WATANABE ,  Toshihiko HOSOYA ,  Minoru KO

IPC分类号： C12N5/079

CPC分类号： C12N5/0622 ,  C12N2501/60 ,  C12N2506/45

摘要： There is provided a production method for microglia, including a step of introducing, into pluripotent stem cells, a nucleic acid including an open reading frame of a transcription factor that increases expression of a Complement C3 (C3) gene. In the production method, the transcription factor is selected from the group consisting of SPI1, CEBPA, PTAFR, FLI1, MEF2C, EGR2, RUNX1, TNF, CEBPB, IKZF1, KLF6, NFIC, ELF4, PAX8, PRDM1, MEF2A, and NR4A3.

公开(公告)号：US20210309958A1

公开(公告)日：2021-10-07

申请号：US16763161

申请日：2018-11-13

申请人： Satoshi IZUMI ,  Manabu SEO ,  Yudai KAWASHIMA ,  Yusuke OSAKI

发明人： Satoshi IZUMI ,  Manabu SEO ,  Yudai KAWASHIMA ,  Yusuke OSAKI

IPC分类号： C12M1/00 ,  C12M1/32 ,  C12Q1/6851

摘要： Provided is a device including at least one well and an amplifiable reagent contained in a specific copy number in the at least one well. In a preferable mode, the device includes information on the specific copy number of the amplifiable reagent. In a more preferable mode, the device includes information on uncertainty as the information on the specific copy number, and the information on uncertainty includes a coefficient of variation CV of the amplifiable reagent, and the coefficient of variation CV satisfies a relational expression: CV

公开(公告)号：US20210147906A1

公开(公告)日：2021-05-20

申请号：US16766117

申请日：2018-11-22

申请人： Yusuke OSAKI ,  Satoshi IZUMI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO ,  Manabu SEO ,  Koei SUZUKI ,  Hirotaka UNNO

发明人： Yusuke OSAKI ,  Satoshi IZUMI ,  Yudai KAWASHIMA ,  Michie HASHIMOTO ,  Manabu SEO ,  Koei SUZUKI ,  Hirotaka UNNO

IPC分类号： C12Q1/6806 ,  B01L3/02 ,  C12Q1/689 ,  C12Q1/70

摘要： Provided is a detection determining method used in detection of a testing target in a sample by amplification of the testing target and an amplifiable reagent, wherein the amplifiable reagent is provided in a number of 200 or less, the detection determining method including a determining step of determining that the testing target is present and a detection result is positive when the amplifiable reagent is amplified and the testing target is amplified, and determining that the testing target is absent or at least less than a specific copy number of the amplifiable reagent and a detection result is negative when the amplifiable reagent is amplified and the testing target is not amplified. Also provided are a detection determining device, a detection determining program, and a device used for the detection determining method.

公开(公告)号：US20190284611A1

公开(公告)日：2019-09-19

申请号：US16356591

申请日：2019-03-18

申请人： YUDAI KAWASHIMA ,  MASAYUKI YUMOTO ,  SATOSHI IZUMI ,  MANABU SEO

发明人： YUDAI KAWASHIMA ,  MASAYUKI YUMOTO ,  SATOSHI IZUMI ,  MANABU SEO

IPC分类号： C12Q1/6806 ,  B01L7/00 ,  B01L3/00 ,  C12Q1/6809 ,  G01N21/64 ,  B41M5/00

摘要： Provided is a nucleic acid sample-contained container including; a first nucleic acid molecule including an intended base sequence and a base sequence for detection different from the intended base sequence; and a second nucleic acid molecule free of the intended base sequence but including the base sequence for detection, wherein the nucleic acid sample-contained container includes the first nucleic acid molecule in a predetermined number. In a preferable mode, the copy number of the intended base sequence is less than 1,000, and the coefficient of variation (CV value) for the copy number is lower than 20%. In a more preferable mode, the nucleic acid molecules are artificially synthesized nucleic acid molecules. In a yet more preferable mode, the first nucleic acid molecule includes the intended base sequence in a plural number in the same molecule.