公开(公告)号：US20240318132A1

公开(公告)日：2024-09-26

申请号：US18733979

申请日：2024-06-05

申请人： Miyaka Ogihara ,  Natsuko Hemmi ,  Naoki Satoh ,  Hidekazu Yaginuma ,  Masayuki Yumoto ,  Toshihiko Hosoya

发明人： Miyaka Ogihara ,  Natsuko Hemmi ,  Naoki Satoh ,  Hidekazu Yaginuma ,  Masayuki Yumoto ,  Toshihiko Hosoya

IPC分类号： C12N5/00 ,  B33Y70/10 ,  C08J3/075 ,  C12N5/0793

CPC分类号： C12N5/0062 ,  B33Y70/10 ,  C08J3/075 ,  C12N5/0619 ,  C08J2389/00

摘要： An object of the present invention is to provide a hydrogel that has both gel shapeability and cell adhesiveness, and enables a long-time cell culture. Provided is a hydrogel (1) including: a tetra-armed polymer (2) containing polyethylene glycol as a backbone, and containing one or more electrophilic functional groups or nucleophilic functional groups in at least one of a side chain and a terminal; and a linear polymer (3) containing two or more nucleophilic functional groups or electrophilic functional groups in at least one of a side chain and a terminal; the tetra-armed polymer (2) and the linear polymer (3) being cross-linked with each other; wherein the hydrogel is characterized in that the linear polymer (3) is protein, peptide, or polysaccharide, that the electrophilic functional group(s) is/are one or more selected from the group consisting of maleimidyl, N-hydroxy-succinimidyl (NHS), sulfosuccinimidyl, phthalimidyl, imidazoyl, acryloyl, and nitrophenyl, and that the nucleophilic functional group(s) is/are one or more selected from the group consisting of thiol, amino, and —CO2PhNO2.

公开(公告)号：US20230323295A1

公开(公告)日：2023-10-12

申请号：US18176959

申请日：2023-03-01

申请人： Masayuki YUMOTO ,  Yudai KAWASHIMA ,  Hikaru WATANABE ,  Toshihiko HOSOYA ,  Minoru KO

发明人： Masayuki YUMOTO ,  Yudai KAWASHIMA ,  Hikaru WATANABE ,  Toshihiko HOSOYA ,  Minoru KO

IPC分类号： C12N5/079

CPC分类号： C12N5/0622 ,  C12N2501/60 ,  C12N2506/45

摘要： There is provided a production method for microglia, including a step of introducing, into pluripotent stem cells, a nucleic acid including an open reading frame of a transcription factor that increases expression of a Complement C3 (C3) gene. In the production method, the transcription factor is selected from the group consisting of SPI1, CEBPA, PTAFR, FLI1, MEF2C, EGR2, RUNX1, TNF, CEBPB, IKZF1, KLF6, NFIC, ELF4, PAX8, PRDM1, MEF2A, and NR4A3.

公开(公告)号：US20190284611A1

公开(公告)日：2019-09-19

申请号：US16356591

申请日：2019-03-18

申请人： YUDAI KAWASHIMA ,  MASAYUKI YUMOTO ,  SATOSHI IZUMI ,  MANABU SEO

发明人： YUDAI KAWASHIMA ,  MASAYUKI YUMOTO ,  SATOSHI IZUMI ,  MANABU SEO

IPC分类号： C12Q1/6806 ,  B01L7/00 ,  B01L3/00 ,  C12Q1/6809 ,  G01N21/64 ,  B41M5/00

摘要： Provided is a nucleic acid sample-contained container including; a first nucleic acid molecule including an intended base sequence and a base sequence for detection different from the intended base sequence; and a second nucleic acid molecule free of the intended base sequence but including the base sequence for detection, wherein the nucleic acid sample-contained container includes the first nucleic acid molecule in a predetermined number. In a preferable mode, the copy number of the intended base sequence is less than 1,000, and the coefficient of variation (CV value) for the copy number is lower than 20%. In a more preferable mode, the nucleic acid molecules are artificially synthesized nucleic acid molecules. In a yet more preferable mode, the first nucleic acid molecule includes the intended base sequence in a plural number in the same molecule.