公开(公告)号：US20110196142A1

公开(公告)日：2011-08-11

申请号：US13062178

申请日：2008-09-04

申请人： Lei Wang ,  YunLiu Fan

发明人： Lei Wang ,  YunLiu Fan

IPC分类号： C07H21/04 ,  C12P19/34

CPC分类号： C12N15/1093 ,  C12N15/10 ,  C12N15/111 ,  C12N15/66 ,  C12N2310/14 ,  C12N2330/31 ,  C12N2330/51

摘要： DNA molecule for expressing hairpin RNA, the constructing method and the use thereof are provided. The DNA molecule is a closed single-strand cyclic DNA molecule which is constructed by linking four fragments A, B, C and D as following order: A-C-B-D or A-D-B-C, wherein A is a sense strand or anti-sense strand fragment of the target double-strand DNA; B is the fragment which is reversely complementary to A; C and D are DNA fragments with stem-loop structure and they meet one of the following requirements: 1) the nucleotide sequence of fragment C or D comprises at least one sequence e; 2) the nucleotide sequence of fragment C comprises at least one sequence e, the nucleotide sequence of fragment D comprises at least one sequence f, wherein sequences e and f individually are one strand of different restriction endonuclease recognition sequences. The DNA molecule for expressing hairpin RNA and its constructing method are useful in constructing lhRNA library.

摘要翻译： 提供用于表达发夹RNA的DNA分子，其构建方法及其用途。 DNA分子是封闭的单链环状DNA分子，其通过以下顺序连接四个片段A，B，C和D来构建：ACBD或ADBC，其中A是靶双链的有义链或反义链片段 品牌DNA; B是与A反向互补的片段; C和D是具有茎环结构的DNA片段，它们满足以下要求之一：1）片段C或D的核苷酸序列包含至少一个序列e; 2）片段C的核苷酸序列包含至少一个序列e，片段D的核苷酸序列包含至少一个序列f，其中序列e和f分别是不同限制性内切核酸酶识别序列的一条链。 用于表达发夹RNA的DNA分子及其构建方法可用于构建lhRNA文库。

公开(公告)号：US20080060098A1

公开(公告)日：2008-03-06

申请号：US11810795

申请日：2007-06-07

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01H5/00 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N5/06

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

公开(公告)号：US07323339B2

公开(公告)日：2008-01-29

申请号：US11046255

申请日：2005-01-28

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01N5/00 ,  C12N15/82 ,  C12N15/87 ,  C12N5/14 ,  C07H21/00

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

摘要翻译： 本发明公开了玉米bZIP转录因子，即ABP2，ABP4和ABP9，编码这些因子的基因及其用途。 转录因子是具有SEQ ID NO：2,4或6所示氨基酸序列的蛋白质，或通过SEQ ID NO：2,4或6所示的氨基酸残基的取代，缺失或添加而得到的蛋白质。 或6，并且具有与SEQ ID NO：2,4或6所示的蛋白质相同的活性。分别编码这些因子的ABP2，ABP4和ABP9基因是具有大于90％同一性的DNA序列， SEQ ID NO：1,3或5所示的序列，以及具有相同功能的编码蛋白质。 这些基因对于对非生物胁迫具有增强的耐受性并且改善植物对非生物胁迫的耐受性的育种植物品种是重要的。

公开(公告)号：US20080060097A1

公开(公告)日：2008-03-06

申请号：US11810784

申请日：2007-06-07

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01H5/00 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N5/06

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

公开(公告)号：US20060021091A1

公开(公告)日：2006-01-26

申请号：US11046255

申请日：2005-01-28

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N5/04 ,  C07H21/04

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factor, the genes encoding these factors and the use thereof. Those transcriptional factors ABP2, ABP4 and ABP9, respectively, are the proteins having the amino acid sequences shown by sequence 2, 4, and 6 in the sequence listing, or the proteins derived from thereof which are produced by substitution, deletion or addition of at least one or more amino acid residues of the amino acid sequence shown by sequence 2, 4 and 6 in the sequence listing, having the same activity as the proteins shown by sequence 2, 4 and 6 in the sequence listing. The genes ABP2, ABP4 and ABP9 encoding these said facters respectively, are the DNA sequences having an identity of more than 90% to the sequences shown by SEQ ID No. 1, 3 and 5 in the sequence listing and the encoded proteins having such same functions. The genes of the invention have important significance for breeding plant varieties with enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

摘要翻译： 本发明公开了玉米bZIP转录因子，编码这些因子的基因及其用途。 那些转录因子ABP2，ABP4和ABP9分别是具有序列表中序列2,4和6所示氨基酸序列的蛋白质，或其衍生自其的蛋白质，其通过置换，缺失或添加产生 序列表中序列2,4和6所示的氨基酸序列的至少一个或多个氨基酸残基具有与序列表中序列2,4和6所示蛋白相同的活性。 分别编码这些所述因子的基因ABP2，ABP4和ABP9分别是与序列表中SEQ ID No.1,3和5所示序列具有超过90％的同一性的DNA序列，并且编码的蛋白具有相同的 功能。 本发明的基因对于对非生物胁迫耐受性增强的植物品种以及提高植物对非生物胁迫的耐受性具有重要意义。