公开(公告)号：US20080060098A1

公开(公告)日：2008-03-06

申请号：US11810795

申请日：2007-06-07

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01H5/00 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N5/06

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

公开(公告)号：US20110196142A1

公开(公告)日：2011-08-11

申请号：US13062178

申请日：2008-09-04

申请人： Lei Wang ,  YunLiu Fan

发明人： Lei Wang ,  YunLiu Fan

IPC分类号： C07H21/04 ,  C12P19/34

CPC分类号： C12N15/1093 ,  C12N15/10 ,  C12N15/111 ,  C12N15/66 ,  C12N2310/14 ,  C12N2330/31 ,  C12N2330/51

摘要： DNA molecule for expressing hairpin RNA, the constructing method and the use thereof are provided. The DNA molecule is a closed single-strand cyclic DNA molecule which is constructed by linking four fragments A, B, C and D as following order: A-C-B-D or A-D-B-C, wherein A is a sense strand or anti-sense strand fragment of the target double-strand DNA; B is the fragment which is reversely complementary to A; C and D are DNA fragments with stem-loop structure and they meet one of the following requirements: 1) the nucleotide sequence of fragment C or D comprises at least one sequence e; 2) the nucleotide sequence of fragment C comprises at least one sequence e, the nucleotide sequence of fragment D comprises at least one sequence f, wherein sequences e and f individually are one strand of different restriction endonuclease recognition sequences. The DNA molecule for expressing hairpin RNA and its constructing method are useful in constructing lhRNA library.

摘要翻译： 提供用于表达发夹RNA的DNA分子，其构建方法及其用途。 DNA分子是封闭的单链环状DNA分子，其通过以下顺序连接四个片段A，B，C和D来构建：ACBD或ADBC，其中A是靶双链的有义链或反义链片段 品牌DNA; B是与A反向互补的片段; C和D是具有茎环结构的DNA片段，它们满足以下要求之一：1）片段C或D的核苷酸序列包含至少一个序列e; 2）片段C的核苷酸序列包含至少一个序列e，片段D的核苷酸序列包含至少一个序列f，其中序列e和f分别是不同限制性内切核酸酶识别序列的一条链。 用于表达发夹RNA的DNA分子及其构建方法可用于构建lhRNA文库。

公开(公告)号：US07323339B2

公开(公告)日：2008-01-29

申请号：US11046255

申请日：2005-01-28

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01N5/00 ,  C12N15/82 ,  C12N15/87 ,  C12N5/14 ,  C07H21/00

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

摘要翻译： 本发明公开了玉米bZIP转录因子，即ABP2，ABP4和ABP9，编码这些因子的基因及其用途。 转录因子是具有SEQ ID NO：2,4或6所示氨基酸序列的蛋白质，或通过SEQ ID NO：2,4或6所示的氨基酸残基的取代，缺失或添加而得到的蛋白质。 或6，并且具有与SEQ ID NO：2,4或6所示的蛋白质相同的活性。分别编码这些因子的ABP2，ABP4和ABP9基因是具有大于90％同一性的DNA序列， SEQ ID NO：1,3或5所示的序列，以及具有相同功能的编码蛋白质。 这些基因对于对非生物胁迫具有增强的耐受性并且改善植物对非生物胁迫的耐受性的育种植物品种是重要的。

公开(公告)号：US20080060097A1

公开(公告)日：2008-03-06

申请号：US11810784

申请日：2007-06-07

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01H5/00 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N5/06

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

公开(公告)号：US20060021091A1

公开(公告)日：2006-01-26

申请号：US11046255

申请日：2005-01-28

申请人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

发明人： Jun Zhao ,  Lei Wang ,  Yunliu Fan

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N5/04 ,  C07H21/04

CPC分类号： C07K14/415 ,  C12N15/8271

摘要： The invention discloses maize bZIP transcriptional factor, the genes encoding these factors and the use thereof. Those transcriptional factors ABP2, ABP4 and ABP9, respectively, are the proteins having the amino acid sequences shown by sequence 2, 4, and 6 in the sequence listing, or the proteins derived from thereof which are produced by substitution, deletion or addition of at least one or more amino acid residues of the amino acid sequence shown by sequence 2, 4 and 6 in the sequence listing, having the same activity as the proteins shown by sequence 2, 4 and 6 in the sequence listing. The genes ABP2, ABP4 and ABP9 encoding these said facters respectively, are the DNA sequences having an identity of more than 90% to the sequences shown by SEQ ID No. 1, 3 and 5 in the sequence listing and the encoded proteins having such same functions. The genes of the invention have important significance for breeding plant varieties with enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

摘要翻译： 本发明公开了玉米bZIP转录因子，编码这些因子的基因及其用途。 那些转录因子ABP2，ABP4和ABP9分别是具有序列表中序列2,4和6所示氨基酸序列的蛋白质，或其衍生自其的蛋白质，其通过置换，缺失或添加产生 序列表中序列2,4和6所示的氨基酸序列的至少一个或多个氨基酸残基具有与序列表中序列2,4和6所示蛋白相同的活性。 分别编码这些所述因子的基因ABP2，ABP4和ABP9分别是与序列表中SEQ ID No.1,3和5所示序列具有超过90％的同一性的DNA序列，并且编码的蛋白具有相同的 功能。 本发明的基因对于对非生物胁迫耐受性增强的植物品种以及提高植物对非生物胁迫的耐受性具有重要意义。

公开(公告)号：US20090000948A1

公开(公告)日：2009-01-01

申请号：US12097409

申请日：2007-01-04

申请人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

发明人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

IPC分类号： G01N27/26 ,  G01N27/00

CPC分类号： C12N13/00 ,  C12M23/16 ,  C12M33/00 ,  C12M35/02

摘要： The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip.

摘要翻译： 本发明提供使用介电电泳辅助细胞定位提高细胞电穿孔效率的方法及其在微流体生物芯片系统中的用途。 细胞首先进行介导电泳，并定位于电场强度足以使细胞电穿孔的区域。 本发明增强了传统微流体生物芯片上原位细胞电穿孔的效率。

公开(公告)号：US20240009411A1

公开(公告)日：2024-01-11

申请号：US18470632

申请日：2023-09-20

申请人： Lei Wang

发明人： Lei Wang

IPC分类号： A61M15/08

CPC分类号： A61M15/08

摘要： A slow release apparatus for slowly releasing a compound in a nasal passage. The apparatus comprises a connection mechanism, two expansion mechanisms, and two release mechanisms; two ends of the connection mechanism are respectively integrally or detachably connected to the two expansion mechanisms that are symmetrically arranged in nasal passages at two sides; and the two release mechanisms are symmetrically arranged, and each release mechanism is correspondingly provided in one of the expansion mechanisms. The slow release apparatus is convenient to carry, and capable of slowly releasing a compound in the nasal passage and synchronously expanding the nasal passage to ensure smooth breathing, while considering functionality, practicability and comfort.

公开(公告)号：USD985256S1

公开(公告)日：2023-05-09

申请号：US29852235

申请日：2022-09-06

申请人： Lei Wang

设计人： Lei Wang

摘要： FIG. 1 is a first perspective view of a walker showing my new design;
FIG. 2 is a second perspective view thereof;
FIG. 3 is a front view thereof;
FIG. 4 is a back view thereof;
FIG. 5 is a left side view thereof;
FIG. 6 is a right side view thereof;
FIG. 7 is a top view thereof; and,
FIG. 8 is a bottom view thereof.
The broken lines shown in the drawings depict portions of the walker that form no part of the claimed design.

公开(公告)号：US11365608B2

公开(公告)日：2022-06-21

申请号：US16148684

申请日：2018-10-01

申请人： Haining Zheng ,  Lei Wang ,  Michael W. Walker

发明人： Haining Zheng ,  Lei Wang ,  Michael W. Walker

IPC分类号： E21B41/00 ,  E21B47/00 ,  E21B44/00 ,  E21B21/08 ,  E21B21/06

摘要： A method of moving a string assembly within a wellbore is disclosed. In some embodiments, the method comprises moving the string assembly within the wellbore; obtaining surface data regarding at least one parameter associated with moving the string assembly within the wellbore over a range of depths; modeling the at least one parameter over the range of depths for a plurality of assumed friction factors to obtain modeled data for each assumed friction factor; calculating a derivative of the surface data over the range of depths; calculating a derivative of the modeled data over the range of depths; comparing the derivative of the surface data to the derivative of the modeled data; determining one or more local friction factors for the range of depths based on the comparison; and adjusting at least one string assembly operating parameter based on the one or more local friction factors.

公开(公告)号：US10352099B2

公开(公告)日：2019-07-16

申请号：US15208704

申请日：2016-07-13

申请人： Benjamin Spivey ,  Gregory S. Payette ,  Darren Pais ,  Krishnan Kumaran ,  Lei Wang ,  Jeffrey R. Bailey ,  Paul E. Pastusek

发明人： Benjamin Spivey ,  Gregory S. Payette ,  Darren Pais ,  Krishnan Kumaran ,  Lei Wang ,  Jeffrey R. Bailey ,  Paul E. Pastusek

IPC分类号： E21B44/02 ,  E21B47/12 ,  E21B47/06 ,  E21B45/00 ,  E21B7/00

摘要： Methods for drilling a wellbore within a subsurface region and drilling assemblies and systems that include and/or utilize the methods are disclosed herein. The methods include receiving a plurality of drilling performance indicator maps, normalizing the plurality of drilling performance indicator maps to generate a plurality of normalized maps, adaptive trending of the plurality of drilling performance indicator maps to generate a plurality of trended maps, summing the plurality of trended maps to generate an objective map, selecting a desired operating regime from the objective map, and adjusting at least one drilling operational parameter of a drilling rig based, at least in part, on the desired operating regime.