公开(公告)号：US20240351041A1

公开(公告)日：2024-10-24

申请号：US18634751

申请日：2024-04-12

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON ,  Justin COSTA ,  Shalini GOHIL ,  Hong CHEN ,  Yufeng QIAN ,  Minkyung PARK ,  Xiaoyang CAI ,  Hiroshi SASAKI ,  Naveed BAKH

IPC: B01L3/00

CPC classification number: B01L3/527 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/12

Abstract: The present disclosure provides systems and methods for reagent storage. System may include one or more substrates comprising a partition. The partition may be configured to retain a first reagent separate from a second reagent. The partition may include a seal disposed between the first and second reagent. The seal may be configured to maintain the first reagent separate from the second reagent. The first and second reagent may be disposed in a first layer and a second layer, respectively, of frozen material. The first layer and second layer of frozen material may maintain the first reagent separate from the second reagent.

公开(公告)号：US20230026886A1

公开(公告)日：2023-01-26

申请号：US17812082

申请日：2022-07-12

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN

IPC: A61L24/00 ,  A61L24/04

Abstract: The present disclosure relates in some aspects to methods for preparing a thin polymer matrix (e.g., a hydrogel matrix) having a biological sample embedded therein for in situ analysis of one or more analytes.

公开(公告)号：US20230015226A1

公开(公告)日：2023-01-19

申请号：US17852250

申请日：2022-06-28

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY ,  Zhao ZHAO

IPC: C12Q1/6841

Abstract: The present application provides methods, compositions, and kits for analyzing a biological sample embedded in a three-dimensional polymerized matrix using a primer-exchange reaction (PER). In some embodiments, the methods comprise contacting the sample with a nucleic acid molecule that directly or indirectly binds to an analyte in the sample and immobilizing the nucleic acid molecule in the matrix, wherein the nucleic acid molecule comprises a free 3′ priming region for initiation of PER. In some embodiments, the methods enable sensitive detection of the identity and relative position of analytes in the sample.

公开(公告)号：US20230323437A1

公开(公告)日：2023-10-12

申请号：US17816991

申请日：2022-08-02

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY ,  Shoshoni DROZ ,  Milena LAZOVA ,  Zhao ZHAO

IPC: C12Q1/6806 ,  C12Q1/6841

CPC classification number: C12Q1/6806 ,  C12Q1/6841

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures such as rolling circle amplification products in the biological sample.

公开(公告)号：US20230013775A1

公开(公告)日：2023-01-19

申请号：US17812075

申请日：2022-07-12

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY

IPC: C12Q1/6841 ,  G01N21/64 ,  G01N33/543

Abstract: The present disclosure relates in some aspects to methods for preparing biological samples for in situ analysis of one or more analytes, wherein the biological sample has been previously affixed to a substrate, which is not compatible with in situ analysis, for example, due to the absence of positional markers and/or fiducial markers and/or a region suitable for in situ signal detection on the substrate.

公开(公告)号：US20240043914A1

公开(公告)日：2024-02-08

申请号：US18366621

申请日：2023-08-07

Applicant: 10X Genomics, Inc.

Inventor： Hong CHEN ,  Joshua Delaney ,  Yi Luo ,  Meiliana Tjandra

IPC: C12Q1/6841 ,  C12Q1/6876 ,  G01N21/64 ,  G01N1/30 ,  C12Q1/6806

CPC classification number: C12Q1/6841 ,  C12Q1/6876 ,  G01N21/64 ,  G01N1/30 ,  C12Q1/6806 ,  G01N2021/6432 ,  G01N2021/6439

Abstract: The present disclosure generally relates to methods and compositions for in situ analysis or detection of analytes in a biological sample. More specifically, the present disclosure relates to methods for reducing autofluorescence in tissue samples, methods for analyzing tissue samples, and compounds for use in the same. The methods and compounds of the present disclosure may be especially suitable for analytical methods employing fluorescence in situ hybridization techniques over multiple cycles of imaging.