公开(公告)号：US12234507B2

公开(公告)日：2025-02-25

申请号：US18366621

申请日：2023-08-07

Applicant: 10x Genomics, Inc.

Inventor： Hong Chen ,  Joshua Delaney ,  Yi Luo ,  Meiliana Tjandra

IPC: C12Q1/6841 ,  C12Q1/6806 ,  C12Q1/6876 ,  G01N1/30 ,  G01N21/64

Abstract: The present disclosure generally relates to methods and compositions for in situ analysis or detection of analytes in a biological sample. More specifically, the present disclosure relates to methods for reducing autofluorescence in tissue samples, methods for analyzing tissue samples, and compounds for use in the same. The methods and compounds of the present disclosure may be especially suitable for analytical methods employing fluorescence in situ hybridization techniques over multiple cycles of imaging.

公开(公告)号：US20240117410A1

公开(公告)日：2024-04-11

申请号：US18499129

申请日：2023-10-31

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini Gohil ,  Veronica Emelina Gonzalez Munoz ,  Joshua Gu ,  Albert Dale Kim ,  Yi Luo ,  Tathagata Mukherjee ,  Monica Nagendran ,  James Francis Perna, III ,  Kristen Nguyen Pham ,  Meiliana Tjandra

IPC: C12Q1/6806 ,  C12Q1/6823

CPC classification number: C12Q1/6806 ,  C12Q1/6823

Abstract: The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.

公开(公告)号：US20240043914A1

公开(公告)日：2024-02-08

申请号：US18366621

申请日：2023-08-07

Applicant: 10X Genomics, Inc.

Inventor： Hong CHEN ,  Joshua Delaney ,  Yi Luo ,  Meiliana Tjandra

IPC: C12Q1/6841 ,  C12Q1/6876 ,  G01N21/64 ,  G01N1/30 ,  C12Q1/6806

CPC classification number: C12Q1/6841 ,  C12Q1/6876 ,  G01N21/64 ,  G01N1/30 ,  C12Q1/6806 ,  G01N2021/6432 ,  G01N2021/6439

Abstract: The present disclosure generally relates to methods and compositions for in situ analysis or detection of analytes in a biological sample. More specifically, the present disclosure relates to methods for reducing autofluorescence in tissue samples, methods for analyzing tissue samples, and compounds for use in the same. The methods and compounds of the present disclosure may be especially suitable for analytical methods employing fluorescence in situ hybridization techniques over multiple cycles of imaging.

公开(公告)号：US12258624B2

公开(公告)日：2025-03-25

申请号：US18499129

申请日：2023-10-31

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Veronica Emelina Gonzalez Muñoz ,  Joshua Gu ,  Albert Dale Kim ,  Yi Luo ,  Tathagata Mukherjee ,  Monica Nagendran ,  James Francis Perna, III ,  Kristen Nguyen Pham ,  Meiliana Tjandra

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6841 ,  C12Q1/6876

Abstract: The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.

公开(公告)号：US12209273B2

公开(公告)日：2025-01-28

申请号：US17346077

申请日：2021-06-11

Applicant: 10x Genomics, Inc.

Inventor： Felice Alessio Bava ,  David M. Patterson ,  Meiliana Tjandra ,  Yi Luo

IPC: C12Q1/6816

Abstract: Provided herein are methods of sequencing comprising click chemistry bioconjugation. In some embodiments, target polynucleotide sequences on the same or different molecules are contacted with and hybridize to probes comprising click functional groups. Probes (e.g., reading probes) hybridizing to adaptor sequences adjacent to different sequences of interest (e.g., barcodes to be sequenced) can be hybridized simultaneously in large pools. In some embodiments, the provided methods achieve multiplexing without requiring separate hybridization of probes (e.g., reading probes) for each sequencing-by-ligation cycle, thereby reducing total hybridization time which is typically a most time-consuming step in in situ technologies. In some aspects, the hybridized probes (e.g., reading probes) are clicked onto detectable probes to analyze a sequence of a target polynucleotide in a sequencing-by-ligation fashion.

公开(公告)号：US20240035071A1

公开(公告)日：2024-02-01

申请号：US18336866

申请日：2023-06-16

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini Gohil ,  Veronica Emelina Gonzalez Muñoz ,  Joshua Gu ,  Albert Dale Kim ,  Yi Luo ,  Tathagata Mukherjee ,  Monica Nagendran ,  James Francis Perna, III ,  Kristen Nguyen Pham ,  Meiliana Tjandra

IPC: C12Q1/6841 ,  C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6841 ,  C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.

公开(公告)号：US20220334031A1

公开(公告)日：2022-10-20

申请号：US17845331

申请日：2022-06-21

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Adam Lowe ,  Yi Luo ,  Dagmar Walter

IPC: G01N1/30 ,  C07D403/12

Abstract: The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.