公开(公告)号：US20240017259A1

公开(公告)日：2024-01-18

申请号：US18373002

申请日：2023-09-26

Applicant: 10x Genomics, Inc.

Inventor： Brenden Janatpour BROWN ,  Ivan AKHREMICHEV ,  Daniel FREITAS ,  Martin SAUZADE

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L2200/16 ,  B01L2200/0673 ,  B01L2400/0457

Abstract: Devices, methods, and systems for generating droplets are provided. The devices, methods, and systems are designed to maximize droplet recovery, e.g., from a collection reservoir.

公开(公告)号：US20220280933A1

公开(公告)日：2022-09-08

申请号：US17751267

申请日：2022-05-23

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Hanyoup KIM ,  Bill Kengli LIN ,  Marissa PENNELL ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER

IPC: B01L3/02 ,  B01L3/00

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. The devices, systems, and methods may include transporting a first liquid through an outlet of a channel and causing relative motion of the outlet and an interface of a second liquid to produce droplets of the first liquid in the second liquid. The devices, systems, and methods may also include illuminating a portion of the liquid as the liquid exits from an outlet. The invention also provides methods, devices, and systems for changing the size of a droplet and for eliminating a droplet from a plurality of droplets.

公开(公告)号：US20210387195A1

公开(公告)日：2021-12-16

申请号：US17458757

申请日：2021-08-27

Applicant: 10x Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Mohammad Rahimi LENJI ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER ,  Yiran ZHANG

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08 ,  B01F5/04

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20230390771A1

公开(公告)日：2023-12-07

申请号：US18237268

申请日：2023-08-23

Applicant: 10x Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Lynna CHEN ,  Martin SAUZADE

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0681 ,  B01L2400/0487

Abstract: The invention provides devices for forming droplets and concentrating droplets, and methods of their use. During operation of the devices, droplets are generated using two liquid phases. Once droplet formation is complete excess continuous phase is removed by the use of one or more pressure differentials.

公开(公告)号：US20220080424A1

公开(公告)日：2022-03-17

申请号：US17357617

申请日：2021-06-24

Applicant: 10X Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Francis CUI ,  Rachel GERVER ,  Mohammad Rahimi LENJI ,  Bill Kengli LIN ,  Anthony MAKAREWICZ ,  Martin SAUZADE ,  Astha TANNA ,  Fernandino VALDECANAS ,  Tobias Daniel WHEELER ,  Yiran ZHANG ,  Alireza SALMANZADEH

IPC: B01L3/00 ,  B01L7/00

Abstract: Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.