公开(公告)号：US20240309448A1

公开(公告)日：2024-09-19

申请号：US18737828

申请日：2024-06-07

Applicant: 10x Genomics, Inc.

Inventor： Katherine PFEIFFER ,  Andrew Scott KOHLWAY ,  Andrew John HILL ,  Paul Eugene LUND

IPC: C12Q1/6874 ,  C12Q1/6818

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240150745A1

公开(公告)日：2024-05-09

申请号：US18481673

申请日：2023-10-05

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY ,  Javelin C. CHI ,  Paul Eugene LUND

IPC: C12N9/00 ,  C07K14/245 ,  C12Q1/6827

CPC classification number: C12N9/93 ,  C07K14/245 ,  C12Q1/6827 ,  C07K2319/21 ,  C07K2319/60 ,  C07K2319/80

Abstract: A recombinant ligase composition including a recombinantly-produced ligase isolated from Acanthocystis turfacea chlorella virus 1 (ATCV-1) fused to at least one polynucleotide-binding polypeptide is provided. Compositions containing the recombinant ligase composition, vectors encoding the recombinant ligase composition, and methods of making and using the recombinant ligase composition are also provided.