公开(公告)号：US20180094291A1

公开(公告)日：2018-04-05

申请号：US15569552

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  EVAN D. BRUTINEL ,  ADAM J. STANENAS

IPC: C12Q1/06 ,  C12M1/12 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12Q1/06 ,  C12M23/04 ,  C12M23/20 ,  C12M23/34 ,  C12M25/06 ,  C12M41/34 ,  C12M41/36

Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and a dry oxygen-scavenging reagent are adhered to the at least one adhesive layer. The oxygen-scavenging reagent consists essentially of particles having a diameter of less than 106 microns.

公开(公告)号：US20170247738A1

公开(公告)日：2017-08-31

申请号：US15593598

申请日：2017-05-12

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： CHIRSTINE A. BINSFELD ,  PATRICK A. MACH ,  MARA S. CELT ,  ADAM J. STANENAS

IPC: C12Q1/10 ,  C12Q1/34

CPC classification number: C12Q1/10 ,  C12Q1/04 ,  C12Q1/34 ,  C12Q2304/00 ,  C12Q2334/00 ,  C12Y302/01023 ,  Y02A50/451

Abstract: A method of detecting a Salmonella microorganism is provided. The method includes the use of a selective growth medium, a first indicator system that is converted to a first detectable product by a Salmonella microorganism, and a second indicator system that is converted to a second detectable product by β-galactosidase enzyme activity. The method further comprises inoculating the growth medium and incubating the inoculated growth medium at a temperature higher than 40 degrees C.

公开(公告)号：US20190194595A1

公开(公告)日：2019-06-27

申请号：US16288712

申请日：2019-02-28

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  MARA S. CELT ,  ADAM J. STANENAS

IPC: C12M1/34 ,  C12M1/00 ,  C12Q1/04 ,  C12Q1/26

CPC classification number: C12M41/36 ,  C12M23/20 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/26 ,  G01N2333/90225 ,  G01N2333/90235

Abstract: A self-contained anaerobic environment-generating culture device is provided. The culture device includes a first substrate having opposing inner and outer surfaces, a second substrate having opposing inner and outer surfaces, a growth region disposed between the inner surfaces of the first and second substrates, an effective amount of a substantially dry enzyme component of an enzyme-mediated oxygen depletion system, an effective amount of a substantially dry enzyme substrate component of the enzyme-mediated oxygen depletion system, and a dry, cold-water-soluble gelling agent disposed in the growth region. The enzyme and enzyme substrate components are disposed in coatings in the growth region. The first and second substrates are substantially nontransmissible to gaseous oxygen. Methods of making and using the culture device area also provided.

公开(公告)号：US20180298322A1

公开(公告)日：2018-10-18

申请号：US15569451

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  EVAN D. BRUTINEL ,  ADAM J. STANENAS

IPC: C12M1/34 ,  C12M1/12 ,  C12M1/00

Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer is adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and an effective amount of a dry carbon dioxide-generating reagent are adhered to the at least one adhesive layer. The carbon dioxide-generating reagent consists essentially of particles having a diameter of less than 106 microns.

公开(公告)号：US20180312895A1

公开(公告)日：2018-11-01

申请号：US15569579

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  EVAN D. BRUTINEL ,  ADAM J. STANENAS

IPC: C12Q1/04 ,  C12M1/34 ,  C12M1/12 ,  C12N1/20 ,  C12P7/56

CPC classification number: C12M25/06 ,  C12M23/04 ,  C12M23/20 ,  C12M41/34 ,  C12M41/36 ,  C12Q1/04

Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.

公开(公告)号：US20170233691A1

公开(公告)日：2017-08-17

申请号：US15503481

申请日：2015-08-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： EVAN D. BRUTINEL ,  JASON W. BJORK ,  ADAM J. STANENAS

IPC: C12M1/26 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/04 ,  C12M1/12

Abstract: The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.

公开(公告)号：US20170191108A1

公开(公告)日：2017-07-06

申请号：US15308622

申请日：2015-05-13

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  ADAM J. STANENAS ,  WENSHENG XIA

IPC: C12Q1/04 ,  C12N1/20 ,  C12Q1/26

CPC classification number: C12Q1/04 ,  C12N1/20 ,  C12Q1/26

Abstract: A culture system for culturing a microaerophilic or an anaerobic microorganism is provided. The culture system can include effective amounts of i) an enzyme of an oxidoreductase family and ii) a substrate for said enzyme, a container, and a predetermined volume of aqueous medium that supports growth of said anaerobic or microaerophilic microorganism. The enzyme can be selected from a group consisting of ascorbic acid oxidase and laccase. The effective amounts are effective to deplete dissolved oxygen in the predetermined volume to a concentration that facilitates growth of a microaerophilic microorganism or an obligately-anaerobic microorganism. A method of using the system is also provided.