公开(公告)号：US20180094291A1

公开(公告)日：2018-04-05

申请号：US15569552

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  EVAN D. BRUTINEL ,  ADAM J. STANENAS

IPC: C12Q1/06 ,  C12M1/12 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12Q1/06 ,  C12M23/04 ,  C12M23/20 ,  C12M23/34 ,  C12M25/06 ,  C12M41/34 ,  C12M41/36

Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and a dry oxygen-scavenging reagent are adhered to the at least one adhesive layer. The oxygen-scavenging reagent consists essentially of particles having a diameter of less than 106 microns.

公开(公告)号：US20180312895A1

公开(公告)日：2018-11-01

申请号：US15569579

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  EVAN D. BRUTINEL ,  ADAM J. STANENAS

IPC: C12Q1/04 ,  C12M1/34 ,  C12M1/12 ,  C12N1/20 ,  C12P7/56

CPC classification number: C12M25/06 ,  C12M23/04 ,  C12M23/20 ,  C12M41/34 ,  C12M41/36 ,  C12Q1/04

Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.

公开(公告)号：US20170233691A1

公开(公告)日：2017-08-17

申请号：US15503481

申请日：2015-08-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： EVAN D. BRUTINEL ,  JASON W. BJORK ,  ADAM J. STANENAS

IPC: C12M1/26 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/04 ,  C12M1/12

Abstract: The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.

公开(公告)号：US20180298322A1

公开(公告)日：2018-10-18

申请号：US15569451

申请日：2016-04-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： JASON W. BJORK ,  EVAN D. BRUTINEL ,  ADAM J. STANENAS

IPC: C12M1/34 ,  C12M1/12 ,  C12M1/00

Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer is adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and an effective amount of a dry carbon dioxide-generating reagent are adhered to the at least one adhesive layer. The carbon dioxide-generating reagent consists essentially of particles having a diameter of less than 106 microns.

公开(公告)号：US20170240949A1

公开(公告)日：2017-08-24

申请号：US15503496

申请日：2015-08-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： EVAN D. BRUTINEL ,  KURT J. HALVERSON

IPC: C12Q1/06 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34

CPC classification number: C12Q1/06 ,  C12M23/34 ,  C12M23/38 ,  C12M25/06 ,  C12M37/04 ,  C12M41/36 ,  C12Q1/04

Abstract: The present disclosure provides a device that includes a base comprising a substrate having a first major surface, a pressure sensitive adhesive adhered to at least a portion of the first major surface, a polymeric cover film coupled to the substrate via the adhesive, a plurality of isolated closed compartments disposed between the substrate and the cover film, and an aqueous liquid disposed in two or more of the closed compartments. The cover film is a composite film comprising ethylene vinyl acetate copolymer, a linear copolymer of ethylene and a higher alkene, and a tackifier. Each compartment of the plurality is defined by a seal that prevents liquid communication with at least one other compartment of the plurality. Methods of using the device for partitioning a sample, for analyzing a sample, and for culturing a microorganism are also provided.