公开(公告)号：US20140243228A1

公开(公告)日：2014-08-28

申请号：US14141498

申请日：2013-12-27

Applicant: AbbVie, Inc.

Inventor： Lorenzo BENATUIL ,  Jennifer PEREZ ,  Chung-Ming HSIEH

IPC: G01N33/68

CPC classification number: G01N33/6854 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/244 ,  C07K16/245 ,  C07K16/468 ,  C07K2317/64 ,  C07K2317/92 ,  C12N15/1037 ,  C40B20/04 ,  C40B30/04

Abstract: System and methods are disclosed for identifying and isolating antibodies with specific affinity with an antigen of interest. Multiple DNA libraries encoding antibodies or their fragments are designed such that the encoded antibodies from different libraries are tagged differently. These libraries may be transformed into yeast cells. The variants of the antibodies are displayed on the surface of the yeast cells and flow cytometry may be used to sort the cells based on antigen affinity and the different tags on the antibodies. By allowing multiple libraries to be screened simultaneously, the disclosed methods help improve the efficiency of affinity.

Abstract translation: 公开了鉴定和分离与感兴趣的抗原具有特异性亲和力的抗体的系统和方法。 设计编码抗体或其片段的多个DNA文库，使得来自不同文库的编码抗体被不同地标记。 这些文库可以转化成酵母细胞。 抗体的变体显示在酵母细胞的表面上，并且流式细胞术可以用于基于抗体的抗原亲和力和不同标签对细胞进行分类。 通过允许同时筛选多个库，所公开的方法有助于提高亲和力的效率。

公开(公告)号：US20140235476A1

公开(公告)日：2014-08-21

申请号：US14141501

申请日：2013-12-27

Applicant: AbbVie, Inc.

Inventor： Jijie GU ,  Maria Cristina HARRIS ,  Lorenzo BENATUIL ,  Chung-Ming HSIEH

IPC: G01N33/566 ,  C07K16/18

CPC classification number: C07K16/18 ,  C07K16/22 ,  C07K16/244 ,  C07K16/245 ,  C07K2317/31 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/626 ,  C07K2317/92 ,  G01N33/6845

Abstract: Provided are protein, nucleic acid, and cellular libraries of multivalent binding proteins (e.g., DVD-Fab or DVD-Ig molecules) and the use of these libraries for the screening of multivalent binding proteins using cell surface display technology (e.g., yeast display).

Abstract translation: 提供了多价结合蛋白（例如，DVD-Fab或DVD-Ig分子）的蛋白质，核酸和细胞文库，以及使用这些文库用于使用细胞表面显示技术（例如酵母展示）筛选多价结合蛋白的方法， 。

公开(公告)号：US20170015742A1

公开(公告)日：2017-01-19

申请号：US15183725

申请日：2016-06-15

Applicant: ABBVIE INC.

Inventor： Jijie GU ,  Diana BOWLEY ,  Lucia EATON ,  Feng DONG ,  Lorenzo BENATUIL ,  Tariq GHAYUR ,  Ravi CHARI ,  Matthew RIESER ,  Anca CLABBERS

IPC: C07K16/22 ,  A61K39/395 ,  C07K16/46 ,  A61K45/06

CPC classification number: C07K16/22 ,  A61K39/3955 ,  A61K45/06 ,  A61K2039/505 ,  C07K14/00 ,  C07K16/24 ,  C07K16/468 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/51 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2317/94

Abstract: Binding proteins that bind one or more of VEGF, PDGF and/or their receptors, including antibodies, CDR-grafted antibodies, humanized antibodies, binding fragments, fusion proteins, and bispecific or multispecific proteins thereof are disclosed. Also disclosed are methods of making and using the binding proteins.

Abstract translation: 公开了结合一种或多种VEGF，PDGF和/或其受体的结合蛋白，包括抗体，CDR-移植的抗体，人源化抗体，结合片段，融合蛋白和双特异性或多特异性蛋白。 还公开了制备和使用结合蛋白的方法。

公开(公告)号：US20170088611A1

公开(公告)日：2017-03-30

申请号：US15252115

申请日：2016-08-30

Applicant: AbbVie Inc.

Inventor： Lorenzo BENATUIL ,  Chung-Ming HSIEH

IPC: C07K16/24 ,  C12N15/10 ,  C07K16/22

CPC classification number: C07K16/245 ,  C07K16/22 ,  C07K16/241 ,  C07K16/244 ,  C07K16/468 ,  C07K2317/35 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/626 ,  C12N15/1082 ,  C40B40/10

Abstract: Provided are protein, nucleic acid, and cellular libraries of single chain multivalent binding proteins (e.g., scDVD and scDVDFab molecules) and methods of using these of these libraries for the screening of single chain multivalent binding proteins using cell surface display technology (e.g., yeast display).

公开(公告)号：US20140221621A1

公开(公告)日：2014-08-07

申请号：US14141500

申请日：2013-12-27

Applicant: AbbVie, Inc.

Inventor： Lorenzo BENATUIL ,  Chung-Ming HSIEH

IPC: C07K16/46 ,  A61K39/395 ,  A61K47/48

CPC classification number: C07K16/245 ,  C07K16/22 ,  C07K16/241 ,  C07K16/244 ,  C07K16/468 ,  C07K2317/35 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/626 ,  C12N15/1082 ,  C40B40/10

Abstract: Provided are protein, nucleic acid, and cellular libraries of single chain multivalent binding proteins (e.g., scDVD and scDVDFab molecules) and methods of using these of these libraries for the screening of single chain multivalent binding proteins using cell surface display technology (e.g., yeast display).

Abstract translation: 提供单链多价结合蛋白（例如scDVD和scDVDFab分子）的蛋白质，核酸和细胞文库以及使用这些文库用于使用细胞表面显示技术筛选单链多价结合蛋白的方法（例如，酵母 显示）。