公开(公告)号：US12221613B2

公开(公告)日：2025-02-11

申请号：US17923455

申请日：2021-04-29

Applicant: Academia Sinica

Inventor： Su-Ying Yeh ,  Hsin-Hung Lin ,  Maurice S. B. Ku ,  Wen-Hsiung Li

IPC: C12N15/82

Abstract: A transgenic rice plant containing in its genome a recombinant DNA construct that includes a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′ untranslated region (5′UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its natural promoter and 5′UTR, the second GLK gene being distinct from the first GLK gene. The first GLK gene and the second GLK gene are both from a C4 plant and the transgenic rice plant exhibits a 65-106% increase in shoot biomass and a 50-95% increase in grain yield, as compared to an untransformed wild-type rice plant. Also provided is a method for producing the transgenic rice plant and a recombinant DNA construct that can be used in the method.

公开(公告)号：US20230203521A1

公开(公告)日：2023-06-29

申请号：US17923455

申请日：2021-04-29

Applicant: Academia Sinica

Inventor： Su-Ying Yeh ,  Hsin-Hung Lin ,  Maurice S. B. Ku ,  Wen-Hsiung Li

IPC: C12N15/82

CPC classification number: C12N15/8269 ,  C12N15/8265

Abstract: A transgenic rice plant containing in its genome a recombinant DNA construct that includes a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′ untranslated region (5′UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its natural promoter and 5′UTR, the second GLK gene being distinct from the first GLK gene. The first GLK gene and the second GLK gene are both from a C4 plant and the transgenic rice plant exhibits a 65-106% increase in shoot biomass and a 50-95% increase in grain yield, as compared to an untransformed wild-type rice plant. Also provided is a method for producing the transgenic rice plant and a recombinant DNA construct that can be used in the method.

公开(公告)号：US11858965B2

公开(公告)日：2024-01-02

申请号：US17327514

申请日：2021-05-21

Applicant: ACADEMIA SINICA

Inventor： Wen-Hsiung Li ,  Anandharaj Marimuthu ,  Jui-Jen Chang ,  Yu-Ju Lin ,  Rizwana Parveen Rani Mohamed Gobil ,  Chieh-Chen Huang

IPC: C07K14/195 ,  C12P7/06 ,  C12P19/02 ,  C12N9/42 ,  C12N9/02 ,  C12P19/14

CPC classification number: C07K14/195 ,  C12N9/0069 ,  C12N9/2437 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  C07K2319/00

Abstract: The present disclosure relates to a recombinant protein comprising a plurality of type II cohesin repeats. The present disclosure provides a recombinant cellulosome complex comprising: the recombinant protein comprising a plurality of type II cohesin repeats; a recombinant cellulosome complex integrating protein A comprising a plurality of type I cohesin repeats, a plurality of cellulose-binding modules and a type II dockerin; and a plurality of recombinant enzymes each comprising a type I dockerin. A cell, a method for digesting a cellulose and a method for producing ethanol are also provided.

公开(公告)号：US11781124B2

公开(公告)日：2023-10-10

申请号：US17094623

申请日：2020-11-10

Applicant: ACADEMIA SINICA

Inventor： Jui-Jen Chang ,  Caroline Thia ,  Hao-Yeh Lin ,  Yu-Ju Lin ,  Chieh-Chen Huang ,  Wen-Hsiung Li

IPC: C12N9/14 ,  C12P23/00 ,  C12N15/81

CPC classification number: C12N9/14 ,  C12N15/815 ,  C12P23/00

Abstract: Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.

公开(公告)号：US10087450B2

公开(公告)日：2018-10-02

申请号：US15156998

申请日：2016-05-17

Applicant: Academia Sinica

Inventor： Jui-Jen Chang ,  Wen-Hsiung Li ,  Chieh-Chen Huang ,  Hao-Yeh Lin ,  Ming-Che Shih

IPC: C12N1/15 ,  C12N15/52 ,  C12N15/65 ,  C12N9/42 ,  C12N15/81 ,  A01H5/02

Abstract: Described herein is an engineered Kluyveromyces marxianus cell, the cell comprising in its genome: (i) two different nucleic acid molecules that each contain a promoter operably linked to a gene encoding a functional enzyme, and (ii) a selection nucleic acid molecule that contains a promoter operably linked to a gene encoding a selectable marker, wherein all of the nucleic acids molecules of (i) and (ii) are in tandem and the engineered cell expresses all of the proteins encoded by the genes of (i) and (ii).

公开(公告)号：US20160251663A1

公开(公告)日：2016-09-01

申请号：US15156998

申请日：2016-05-17

Applicant: Academia Sinica

Inventor： Jui-Jen Chang ,  Wen-Hsiung Li ,  Chieh-Chen Huang ,  Hao-Yeh Lin ,  Ming-Che Shih

IPC: C12N15/52 ,  C12N9/42 ,  C07K14/395 ,  C12N15/65

CPC classification number: C12N15/52 ,  A01H5/02 ,  C12N9/2437 ,  C12N9/2445 ,  C12N15/65 ,  C12N15/815 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091

Abstract: Described herein is an engineered Kluyveromyces marxianus cell, the cell comprising in its genome: (i) two different nucleic acid molecules that each contain a promoter operably linked to a gene encoding a functional enzyme, and (ii) a selection nucleic acid molecule that contains a promoter operably linked to a gene encoding a selectable marker, wherein all of the nucleic acids molecules of (i) and (ii) are in tandem and the engineered cell expresses all of the proteins encoded by the genes of (i) and (ii).

Abstract translation: 本文描述的是一种工程化的克鲁维酵母克氏乳杆菌细胞，该细胞在其基因组中包含：（i）两种不同的核酸分子，每种核酸分子含有可操作地连接到编码功能性酶的基因的启动子，和（ii）选择性核酸分子， 与编码可选择标记的基因可操作地连接的启动子，其中（i）和（ii）的所有核酸分子是串联的，并且工程化细胞表达由（i）和（ii）的基因编码的所有蛋白质 ）。