公开(公告)号：US20170121752A1

公开(公告)日：2017-05-04

申请号：US15311185

申请日：2015-05-14

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Yin Nah TEO ,  Zhi Yi LEE ,  Farid GHADESSY ,  Sarada Srinivasa RAGHAVAN

IPC: C12Q1/02 ,  C09K11/06 ,  G01N21/64

CPC classification number: C12Q1/02 ,  C07D257/04 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1044 ,  G01N21/6428 ,  G01N21/77 ,  G01N2021/6432 ,  G01N2021/7786

Abstract: There is provided a method for detecting the presence or absence of acrylic acid or its derivatives thereof in a sample, the method comprising the steps of: (a) introducing a probe comprising a diaryltetrazole compound to the sample; (b) exposing said sample to light; and (c) detecting the presence or absence of acrylic acid or its derivatives thereof in the sample based on fluorescence emitted by the sample after step (c).

公开(公告)号：US20180172678A1

公开(公告)日：2018-06-21

申请号：US15382570

申请日：2016-12-16

Applicant: Agency for Science, Technology and Research ,  National University of Singapore

Inventor： Yen Nee TAN ,  Xinting ZHENG ,  Farid GHADESSY ,  Walter Leng Peng GOH ,  David LANE ,  Lin-Yue Lanry YUNG

IPC: G01N33/53 ,  G01N33/574

CPC classification number: G01N33/6872 ,  C07K14/4705 ,  C12Q1/6816 ,  G01N33/54346 ,  G01N2015/0222 ,  G01N2500/02 ,  G01N2500/20 ,  C12Q2522/101 ,  C12Q2563/137 ,  C12Q2563/155 ,  C12Q2565/531 ,  C12Q2565/628

Abstract: Methods, systems and nanoprobes for identifying sequence-specific transcription factor DNA interactions for drug and/or disease screening are provided. The method includes homogeneously mixing plasmonic metal nanoparticle probes with protein samples as an assay in multi-well plates. The plasmonic metal nanoparticle probes comprise a plurality of plasmonic metal nanoparticles and a specific DNA response element and the protein samples bind with the specific response elements to form an assembly of the plasmonic metal nanoparticle probes. The method further includes measuring particle size distribution of the assembly of the plasmonic metal nanoparticle probes in the solution by dynamic light scattering and determining one or more sequence-specific transcription factor DNA interactions from a curve of the particle size distribution determined from light scattered by the dynamic light scattering. The nanoprobe includes a plurality of plasmonic metal nanoparticles and a DNA linker. The DNA linker forms a link between the two plasmonic metal nanoparticles, the DNA linker including a double stranded region encoding a specific response element.

公开(公告)号：US20200063165A1

公开(公告)日：2020-02-27

申请号：US16430410

申请日：2019-06-03

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid GHADESSY ,  Jia Wei SLAU ,  Peter DROGE ,  Harshyaa MAKHIJA ,  Shree Harsh VIJAYA CHANDRA ,  Sabrina PETER

IPC: C12N15/90 ,  C12N9/22 ,  C12N9/12

Abstract: The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.

公开(公告)号：US20170327847A1

公开(公告)日：2017-11-16

申请号：US15502783

申请日：2015-08-11

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid GHADESSY ,  Jia Wei SIAU ,  Peter DROGE ,  Harshyaa MAKHIJA ,  Shree Harsh VIJAYA CHANDRA ,  Sabrina PETER

IPC: C12N15/90 ,  C12N9/12

CPC classification number: C12N15/907 ,  C12N9/1241 ,  C12N9/22 ,  C12N15/90 ,  C12N2795/10322

Abstract: The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.