公开(公告)号：US20240366783A1

公开(公告)日：2024-11-07

申请号：US18666145

申请日：2024-05-16

Applicant: AJINOMOTO CO., INC.

Inventor： Kei YAMADA ,  Yutaka MATSUDA ,  Tomohiro FUJII ,  Natsuki SHIKIDA ,  Reiko YUJI ,  Kazutaka SHIMBO ,  Yuji ITO

IPC: A61K47/68 ,  C07K16/00 ,  C07K19/00

Abstract: The present invention provides a technique enabling modification of a soluble protein and in particular regioselective modification of a soluble protein. More specifically, the present invention provides a compound having an affinity substance to a soluble protein, a cleavable portion, and a reactive group represented by the following Formula (I): A-L-B-R  (I) wherein A is an affinity substance to a soluble protein; L is a cleavable linker which is a divalent group comprising a cleavable portion; B is (a) a divalent group comprising a bioorthogonal functional group or (b) a divalent group comprising no bioorthogonal functional group; and R is a reactive group to the soluble protein; or a salt thereof.

公开(公告)号：US20210139549A1

公开(公告)日：2021-05-13

申请号：US17119786

申请日：2020-12-11

Applicant: AJINOMOTO CO., INC.

Inventor： Kei YAMADA ,  Tomohiro FUJII ,  Natsuki SHIKIDA ,  Kazutaka SHIMBO

IPC: C07K14/47 ,  C07K16/00

Abstract: Compounds having an affinity substance to an antibody, a cleavable portion, and a reactive group, represented by the following Formula (I): A-L-B-R  (I) wherein A is the affinity substance an antibody, L is a cleavable linker which is a divalent group comprising the cleavable portion, B is (a) a divalent group comprising a bioorthogonal functional group or (b) a divalent group comprising no bioorthogonal functional group, and R is the reactive group to the antibody, in which the affinity substance to an antibody is a certain peptide, or a salt thereof are useful for the modification of an antibody, particularly the regioselective modification of an antibody.

公开(公告)号：US20240058473A1

公开(公告)日：2024-02-22

申请号：US18467134

申请日：2023-09-14

Applicant: Ajinomoto Co., Inc.

Inventor： Kei YAMADA ,  Yutaka MATSUDA ,  Ippei INOUE ,  Natsuki SHIKIDA ,  Kazutaka SHIMBO

IPC: A61K47/69 ,  A61K47/68

CPC classification number: A61K47/6949 ,  A61K47/6835 ,  A61K47/6889

Abstract: The present invention provides a highly controlled complex comprising an antibody and ferritin particles. More specifically, the present invention provides the inventions of a complex or salt thereof comprising (A) one IgG antibody and (B) two human ferritin particles comprising one or more human ferritin H chains linked thereto; and methods for producing the same.

公开(公告)号：US20230279063A1

公开(公告)日：2023-09-07

申请号：US18062906

申请日：2022-12-07

Applicant: Ajinomoto Co., Inc.

Inventor： Kei YAMADA ,  Ippei INOUE ,  Natsuki SHIKIDA ,  Kazutaka SHIMBO

IPC: C07K14/47 ,  A61K47/64

CPC classification number: C07K14/47 ,  A61K47/64

Abstract: Controlling the number of modifying groups introduced into a surface of ferritin affords a modified ferritin containing a human ferritin H chain, in which the human ferritin H chain contains a modifying group that is covalently bonded specifically to a cysteine residue at position 91 and/or position 103 according to a reference position of a natural human ferritin H chain.

公开(公告)号：US20210300972A1

公开(公告)日：2021-09-30

申请号：US17241585

申请日：2021-04-27

Applicant: Ajinomoto Co., Inc.

Inventor： Kei YAMADA ,  Yoshihiko MATSUDA ,  Kazutoshi TAKAHASHI ,  Natsuki SHIKIDA ,  Kazutaka SHIMBO ,  Hayato NAGANO

IPC: C07K14/31 ,  C07K16/32

Abstract: Compounds having an affinity substance to an antibody, a cleavable portion, and a reactive group, or a salt thereof, are represented by the following Formula (I): A-L-B—R  (I) wherein A is an affinity substance to an antibody, L is a cleavable linker that is a divalent group comprising a cleavable portion, B is (a) a divalent group comprising a bioorthogonal functional group or (b) a divalent group comprising no bioorthogonal functional group, and R is a reactive group to the antibody, wherein the affinity substance to an antibody is a polypeptide comprising a glutamine residue (Q) at an N-terminal.

公开(公告)号：US20210139541A1

公开(公告)日：2021-05-13

申请号：US17119495

申请日：2020-12-11

Applicant: Ajinomoto Co., Inc.

Inventor： Yutaka MATSUDA ,  Noriko HATADA ,  Kei YAMADA ,  Natsuki SHIKIDA ,  Kazutaka SHIMBO ,  Tomohiro FUJII ,  Shigeo HIRASAWA

IPC: C07K14/00 ,  C07K16/00 ,  C07K1/06

Abstract: Compounds having an affinity substance to an antibody and a bioorthogonal functional group, represented by the following Formula (I): A-L-E-B  (I) wherein A is an affinity substance to an antibody, L is a divalent group comprising a leaving group, E is a divalent group comprising an electrophilic group (i) coupled with the leaving group and (ii) having ability to react with a nucleophilic group in the antibody, B is a bioorthogonal functional group, and the leaving group has ability to be cleaved and eliminated from E by a reaction between the nucleophilic group and the electrophilic group, or a salt thereof, and the like are useful for labelling antibodies.