公开(公告)号：US20160312254A1

公开(公告)日：2016-10-27

申请号：US15097632

申请日：2016-04-13

申请人： AJINOMOTO CO., INC.

发明人： Christina Dmitrievna Hook ,  Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova ,  Tatiyana Sergeevna Sidorova

IPC分类号： C12P13/06 ,  C07K14/245

CPC分类号： C12P13/06 ,  C07K14/245

摘要： The present invention provides a method for producing L-isoleucine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress the cycA gene. The method also allows reducing the amount of byproduct amino acids that are produced during fementative production of L-isoleucine using Enterobacteriaceae bacterium having an L-isoleucine-producing ability.

摘要翻译： 本发明提供了使用已经被修饰为过表达cycA基因的肠杆菌科的细菌通过发酵生产L-异亮氨酸的方法。 该方法还可以减少使用具有L-异亮氨酸生产能力的肠杆菌科细菌产生L-异亮氨酸的产物中副产物氨基酸的量。

公开(公告)号：US09896704B2

公开(公告)日：2018-02-20

申请号：US15097632

申请日：2016-04-13

申请人： AJINOMOTO CO., INC.

发明人： Christina Dmitrievna Hook ,  Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova ,  Tatiyana Sergeevna Sidorova

IPC分类号： C12P13/06 ,  C07K14/245

CPC分类号： C12P13/06 ,  C07K14/245

摘要： The present invention provides a method for producing L-isoleucine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress the cycA gene. The method also allows reducing the amount of byproduct amino acids that are produced during fementative production of L-isoleucine using Enterobacteriaceae bacterium having an L-isoleucine-producing ability.

公开(公告)号：US11746345B2

公开(公告)日：2023-09-05

申请号：US15165606

申请日：2016-05-26

申请人： AJINOMOTO CO., INC.

发明人： Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova

IPC分类号： C12N9/00 ,  C12P13/08 ,  C12P13/04 ,  C12N15/52 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/22 ,  C12P13/24

CPC分类号： C12N9/93 ,  C12N15/52 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y603/02002

摘要： The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.

公开(公告)号：US09574216B2

公开(公告)日：2017-02-21

申请号：US14499655

申请日：2014-09-29

申请人： AJINOMOTO CO., INC.

发明人： Elena Viktorovna Sycheva ,  Valery Vasilievich Samsonov ,  Ekaterina Alekseevna Savrasova ,  Natalia Sergeevna Eremina ,  Natalia Vladimirovna Geraskina ,  Natalia Viktorovna Stoynova

IPC分类号： C12P13/08 ,  C12N15/63 ,  C12N15/70 ,  C12P13/06

CPC分类号： C12P13/08 ,  C12N15/63 ,  C12N15/70 ,  C12P13/06

摘要： The present invention describes a method for producing a useful metabolite using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain a gene(s) expression system including elements of the LysR-type protein-regulated transcriptional machinery modified in such a way that auto-inducible positive feedback regulation of the system is mediated by a coinducer. The method is suitable for producing branched-chain L-amino acids, particularly L-valine, L-isoleucine and L-leucine; and D-pantothenic acid.

摘要翻译： 本发明描述了使用肠杆菌科，特别是属于埃希氏杆菌属的细菌的细菌来生产有用的代谢物的方法，该方法已被修饰为含有包含LysR型蛋白质的元件的基因表达系统， 修改的调节转录机制使得该系统的自动诱导性正反馈调节由引导器介导。 该方法适用于生产支链L-氨基酸，特别是L-缬氨酸，L-异亮氨酸和L-亮氨酸; 和D-泛酸。

公开(公告)号：US20150017693A1

公开(公告)日：2015-01-15

申请号：US14499655

申请日：2014-09-29

申请人： AJINOMOTO CO., INC.

发明人： Elena Viktorovna Sycheva ,  Valery Vasilievich Samsonov ,  Ekaterina Alekseevna Savrasova ,  Natalia Sergeevna Eremina ,  Natalia Vladimirovna Geraskina ,  Natalia Viktorovna Stoynova

IPC分类号： C12P13/08 ,  C12N15/70

CPC分类号： C12P13/08 ,  C12N15/63 ,  C12N15/70 ,  C12P13/06

摘要： The present invention describes a method for producing a useful metabolite using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain a gene(s) expression system including elements of the LysR-type protein-regulated transcriptional machinery modified in such a way that auto-inducible positive feedback regulation of said system is mediated by a coinducer. The method is suitable for producing branched-chain L-amino acids, particularly L-valine, L-isoleucine and L-leucine; and D-pantothenic acid.

摘要翻译： 本发明描述了使用肠杆菌科，特别是属于埃希氏杆菌属的细菌的细菌来生产有用的代谢物的方法，该方法已被修饰为含有包含LysR型蛋白质的元件的基因表达系统， 调节的转录机制以使得所述系统的自动诱导性正反馈调节由制裁者介导的方式修改。 该方法适用于生产支链L-氨基酸，特别是L-缬氨酸，L-异亮氨酸和L-亮氨酸; 和D-泛酸。

公开(公告)号：US20220213515A1

公开(公告)日：2022-07-07

申请号：US17703519

申请日：2022-03-24

申请人： AJINOMOTO CO., INC.

发明人： Natalia Viktorovna Stoynova ,  Elena Viktorovna Sycheva ,  Anna Alexandrovna Ublinskaya ,  Valery Vasilievich Samsonov ,  Alexandr Dmitrievich Kivero

IPC分类号： C12P7/52 ,  C12N9/10 ,  C12N9/88 ,  C12N9/04

摘要： The present invention provides a method for producing 2-methyl-butyric acid by fermentation using a bacterium belonging to the order Enterobacterales which has been modified to attenuate expression of a tyrB gene encoding a protein having tyrosine aminotransferase activity. The method also allows for production of a byproduct substance of 2-methyl-butyric acid during fermentation of the Enterobacterales bacterium having 2-methyl-butyric acid-producing ability.

公开(公告)号：US20160348089A1

公开(公告)日：2016-12-01

申请号：US15165606

申请日：2016-05-26

申请人： AJINOMOTO CO., INC.

发明人： Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova

IPC分类号： C12N9/00 ,  C12P13/24 ,  C12P13/08 ,  C12N15/52 ,  C12P13/12 ,  C12P13/14 ,  C12P13/10 ,  C12P13/22 ,  C12P13/06

CPC分类号： C12N9/93 ,  C12N15/52 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y603/02002

摘要： The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.

摘要翻译： 本发明提供了使用肠杆菌科的细菌，特别是属于埃希氏杆菌属的细菌的发酵，通过发酵来生产L-氨基酸的支链L-氨基酸的方法，所述细菌已被修饰以减弱表达 gshA基因。

公开(公告)号：US20160340681A1

公开(公告)日：2016-11-24

申请号：US15233258

申请日：2016-08-10

申请人： AJINOMOTO CO., INC.

发明人： Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova ,  Vera Georgievna Doroshenko

IPC分类号： C12N15/70 ,  C12P13/22 ,  C12P13/24 ,  C12P13/12 ,  C12P13/14 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10

CPC分类号： C12N15/70 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24

摘要： The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.

摘要翻译： 本发明提供了通过使用肠杆菌科，特别是已经修饰为过表达yajL基因的属于埃希氏杆菌属的细菌的细菌进行发酵来生产L-氨基酸或其盐的方法。

公开(公告)号：US09493777B1

公开(公告)日：2016-11-15

申请号：US15233258

申请日：2016-08-10

申请人： AJINOMOTO CO., INC.

发明人： Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova ,  Vera Georgievna Doroshenko

IPC分类号： C12P13/22 ,  C12N15/70 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/06 ,  C12P13/24

CPC分类号： C12N15/70 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24

摘要： The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.

摘要翻译： 本发明提供了通过使用肠杆菌科，特别是已经修饰为过表达yajL基因的属于埃希氏杆菌属的细菌的细菌进行发酵来生产L-氨基酸或其盐的方法。

公开(公告)号：US09175319B2

公开(公告)日：2015-11-03

申请号：US14259365

申请日：2014-04-23

申请人： AJINOMOTO CO., INC.

发明人： Natalia Viktorovna Stoynova ,  Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Evgeniya Aleksandrovna Polyakova

IPC分类号： C12P13/04 ,  C12P13/22 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/24

CPC分类号： C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/22 ,  C12P13/222 ,  C12P13/225 ,  C12P13/227 ,  C12P13/24

摘要： The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.

摘要翻译： 本发明提供一种通过发酵使用肠杆菌科，特别是属于埃希氏杆菌属的细菌的细菌来生产L-氨基酸的方法，其已经被修饰以减弱yjjK基因的表达。