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1.发明申请公开(公告)号：US20150307899A1
公开(公告)日：2015-10-29
申请号：US14649500
申请日：2013-12-06
IPC分类号： C12N15/86 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07H21/04 , C07K14/005 , C12N7/00 , C12N15/85 , C12N2502/00 , C12N2510/00 , C12N2710/14022 , C12N2710/14023 , C12N2710/14034 , C12N2710/14041 , C12N2710/14122 , C12N2710/14131 , C12N2710/14143 , C12N2710/20022 , C12N2710/20034 , C12N2710/20051 , C12N2750/10022 , C12N2750/10034 , C12N2750/10051 , C12N2770/16022 , C12N2770/16034 , C12N2770/16051
摘要： The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.
摘要翻译： 本发明可以包括在生物技术领域中，其涵盖了通过新颖的表达盒在昆虫细胞或昆虫幼虫中作为生物物质改进重组蛋白的生产。 该表达盒包括核酸序列，例如启动子，作为增强子的同源区（hr）和编码转录调节子的序列，例如杆状病毒Ac-ie-01 cDNA或其任何组合，其能够提高质量和 重组蛋白的生产效率。 此外，本发明还涉及包含本发明的上述核酸序列的载体本身，用那些序列或载体感染，转化或转染的细胞或昆虫，以及通过使用上述序列产生重组蛋白的方法， 载体，细胞或昆虫。
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公开(公告)号：US09879280B2
公开(公告)日：2018-01-30
申请号：US14649500
申请日：2013-12-06
IPC分类号： C07H21/04 , C12N5/00 , C12N7/00 , C12N15/00 , C12P21/06 , C12N15/86 , C07K14/005 , C12N15/85
CPC分类号： C12N15/86 , C07H21/04 , C07K14/005 , C12N7/00 , C12N15/85 , C12N2502/00 , C12N2510/00 , C12N2710/14022 , C12N2710/14023 , C12N2710/14034 , C12N2710/14041 , C12N2710/14122 , C12N2710/14131 , C12N2710/14143 , C12N2710/20022 , C12N2710/20034 , C12N2710/20051 , C12N2750/10022 , C12N2750/10034 , C12N2750/10051 , C12N2770/16022 , C12N2770/16034 , C12N2770/16051
摘要： The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.
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