公开(公告)号：US20190153496A1

公开(公告)日：2019-05-23

申请号：US16261311

申请日：2019-01-29

Applicant: AMGEN INC.

Inventor： Shivani GUPTA ,  Sohye KANG

IPC: C12P21/00 ,  C07K16/00 ,  C12N9/10 ,  C07K14/705 ,  C12N15/85

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

公开(公告)号：US20200277642A1

公开(公告)日：2020-09-03

申请号：US16875832

申请日：2020-05-15

Applicant: AMGEN INC.

Inventor： Shivani GUPTA ,  Sohye KANG

IPC: C12P21/00 ,  C12N9/10 ,  C12N15/85 ,  C07K14/705 ,  C07K16/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

公开(公告)号：US20200071738A1

公开(公告)日：2020-03-05

申请号：US16676340

申请日：2019-11-06

Applicant: Amgen Inc.

Inventor： Sohye KANG ,  Chung HUANG, JR. ,  Hedieh BARKHORDARIAN ,  Pavel BONDARENKO ,  Zhongqi ZHANG

IPC: C12P21/00 ,  C07K16/00 ,  C07K14/52 ,  C12N5/071 ,  C12N1/38

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US20170166942A1

公开(公告)日：2017-06-15

申请号：US15115615

申请日：2014-12-11

Applicant: AMGEN INC.

Inventor： Shivani GUPTA ,  Sohye KANG

IPC: C12P21/00 ,  C07K14/705 ,  C07K16/00 ,  C12N9/10

CPC classification number: C12P21/005 ,  C07K14/705 ,  C07K16/00 ,  C07K2317/41 ,  C12N9/1051 ,  C12Y204/01101 ,  C12Y204/01143

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

公开(公告)号：US20190010532A1

公开(公告)日：2019-01-10

申请号：US16130879

申请日：2018-09-13

Applicant: AMGEN INC.

Inventor： Shivani GUPTA ,  Sohye KANG

IPC: C12P21/00 ,  C07K16/00 ,  C07K14/705 ,  C12N9/10

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

公开(公告)号：US20160333385A1

公开(公告)日：2016-11-17

申请号：US15111470

申请日：2014-12-09

Applicant: AMGEN INC.

Inventor： Sohye KANG ,  Chung HUANG, Jr. ,  Hedieh BARKHORDARIAN ,  Pavel BONDARENKO ,  Zhongqi ZHANG

IPC: C12P21/00 ,  C12N5/071 ,  C07K14/52 ,  C07K16/00

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

Abstract translation: 本发明涉及通过调节细胞培养过程中的鸟氨酸代谢来操纵重组糖蛋白的高甘露糖糖型含量的方法。