公开(公告)号：US20210230234A1

公开(公告)日：2021-07-29

申请号：US17221371

申请日：2021-04-02

Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

Inventor： Penelope R. CHUA ,  Hanxiao JIANG ,  Adam Leon MEADOWS

IPC: C07K14/395 ,  C12P19/62 ,  C12P5/02 ,  C12P7/64 ,  C12P21/00 ,  C12N1/16 ,  C12N15/52 ,  C12N15/63 ,  C12N15/65 ,  C12P21/02

Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

公开(公告)号：US20150093797A1

公开(公告)日：2015-04-02

申请号：US14474976

申请日：2014-09-02

Applicant: AMYRIS, INC.

Inventor： Timothy Stevens GARDNER ,  Kristy Michelle HAWKINS ,  Adam Leon MEADOWS ,  Annie Ening TSONG ,  Yoseph TSEGAYE

IPC: C12P5/00 ,  C12N15/81

CPC classification number: C12P5/007 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1288 ,  C12N15/52 ,  C12N15/81 ,  C12P7/42 ,  C12P23/00 ,  C12Y101/01034 ,  C12Y102/0101 ,  C12Y207/08

Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.

Abstract translation: 本文提供了在宿主细胞中异源产生乙酰辅酶A衍生的类异戊二烯的组合物和方法。 在一些实施方案中，宿主细胞被遗传修饰以包含编码乙醛脱氢酶，乙酰化（ADA，E.C.1.2.1.10）和包含使用NADH的HMG-CoA还原酶的MEV途径的异源核苷酸序列。 在一些实施方案中，宿主细胞被遗传修饰以包含编码包含乙酰乙酰辅酶A合成酶的ADA和MEV途径的异源核苷酸序列。 在一些实施方案中，经遗传修饰的宿主细胞还包含一个或多个编码磷酸酮醇酶和磷酸转乙酰酶的异源核苷酸序列。 在一些实施方案中，遗传修饰的宿主细胞还包括天然PDH旁路的功能性破坏。 本文描述的组合物和方法提供了用于异源产生乙酰辅酶A衍生的类异戊二烯的能量效率尚未的氧化还原平衡途径。

公开(公告)号：US20130236942A1

公开(公告)日：2013-09-12

申请号：US13752293

申请日：2013-01-28

Applicant: AMYRIS, INC.

Inventor： Timothy Stevens GARDNER ,  Kristy Michelle HAWKINS ,  Adam Leon MEADOWS ,  Annie Ening TSONG ,  Yoseph TSEGAYE

IPC: C12P5/00 ,  C12N15/81

CPC classification number: C12P5/007 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1288 ,  C12N15/52 ,  C12N15/81 ,  C12P7/42 ,  C12P23/00 ,  C12Y101/01034 ,  C12Y102/0101 ,  C12Y207/08

Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.

公开(公告)号：US20180186841A1

公开(公告)日：2018-07-05

申请号：US15738555

申请日：2016-06-24

Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

Inventor： Penelope R. CHUA ,  Hanxiao JIANG ,  Adam Leon MEADOWS

IPC: C07K14/395 ,  C12P5/02

Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

公开(公告)号：US20180171341A1

公开(公告)日：2018-06-21

申请号：US15738918

申请日：2016-06-24

Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

Inventor： Penelope R. CHUA ,  Hanxiao JIANG ,  Adam Leon MEADOWS

IPC: C12N15/63 ,  C12P21/02 ,  C12P5/02 ,  C12P7/64 ,  C12N1/16 ,  C12N15/52 ,  C12P19/62 ,  C12N15/65

CPC classification number: C07K14/395 ,  C07K2319/95 ,  C12N1/16 ,  C12N15/52 ,  C12N15/635 ,  C12N15/65 ,  C12N2330/51 ,  C12P5/02 ,  C12P5/026 ,  C12P7/64 ,  C12P19/62 ,  C12P21/00 ,  C12P21/02 ,  C12Y503/03002

Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.