公开(公告)号：US20220106619A1

公开(公告)日：2022-04-07

申请号：US17425634

申请日：2020-01-23

Applicant: AMYRIS, INC.

Inventor： Gale A. WICHMANN ,  Sean LUND ,  Joshua LERMAN ,  Hanxiao JIANG ,  Yi XIONG

IPC: C12P19/56 ,  C12N9/10 ,  C12N9/90 ,  C12N9/02 ,  C12N9/88 ,  C12P7/40 ,  C12P5/00

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleic acid expression cassette that expresses an ABC-transporter capable of transporting steviol glycosides to the extracellular space or to the luminal space of an intracellular organelle. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20210371892A1

公开(公告)日：2021-12-02

申请号：US17285888

申请日：2019-10-14

Applicant: AMYRIS, INC.

Inventor： Gale A. WICHMANN ,  Wenzong LI ,  Sean LUND ,  Shaina J. JACKSON ,  Chantal V. GARCIA DE GONZALO ,  Hailley WARBINGTON ,  Yi XIONG ,  Svetlana Alekseyevna BORISOVA

IPC: C12P19/56 ,  C12N9/02 ,  C12N9/10 ,  C12N1/18 ,  C12N9/88 ,  C12N9/90

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Stevia rebaudiana kaurenoic acid hydroxylase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.