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    METHODS FOR STABILIZING PRODUCTION OF ACETYL-COENZYME A DERIVED COMPOUNDS
    2.
    发明申请
    METHODS FOR STABILIZING PRODUCTION OF ACETYL-COENZYME A DERIVED COMPOUNDS 有权

    公开(公告)号：US20210155939A1

    公开(公告)日：2021-05-27

    申请号：US17159797

    申请日：2021-01-27

    Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

    Inventor： Hanxiao JIANG ,  Adam MEADOWS

    IPC: C12N15/52 ,  C12P5/00 ,  C12N1/20 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90

    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

    ABC TRANSPORTERS FOR THE HIGH EFFICIENCY PRODUCTION OF REBAUDIOSIDES
    4.
    发明申请
    ABC TRANSPORTERS FOR THE HIGH EFFICIENCY PRODUCTION OF REBAUDIOSIDES 有权

    公开(公告)号：US20220106619A1

    公开(公告)日：2022-04-07

    申请号：US17425634

    申请日：2020-01-23

    Applicant: AMYRIS, INC.

    Inventor： Gale A. WICHMANN ,  Sean LUND ,  Joshua LERMAN ,  Hanxiao JIANG ,  Yi XIONG

    IPC: C12P19/56 ,  C12N9/10 ,  C12N9/90 ,  C12N9/02 ,  C12N9/88 ,  C12P7/40 ,  C12P5/00

    Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleic acid expression cassette that expresses an ABC-transporter capable of transporting steviol glycosides to the extracellular space or to the luminal space of an intracellular organelle. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

    MALTOSE DEPENDENT DEGRONS, MALTOSE-RESPONSIVE PROMOTERS, STABILIZATION CONSTRUCTS, AND THEIR USE IN PRODUCTION OF NON-CATABOLIC COMPOUNDS
    8.
    发明申请
    MALTOSE DEPENDENT DEGRONS, MALTOSE-RESPONSIVE PROMOTERS, STABILIZATION CONSTRUCTS, AND THEIR USE IN PRODUCTION OF NON-CATABOLIC COMPOUNDS 审中-公开

    公开(公告)号：US20180186841A1

    公开(公告)日：2018-07-05

    申请号：US15738555

    申请日：2016-06-24

    Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

    Inventor： Penelope R. CHUA ,  Hanxiao JIANG ,  Adam Leon MEADOWS

    IPC: C07K14/395 ,  C12P5/02

    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

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