公开(公告)号：US20240174987A1

公开(公告)日：2024-05-30

申请号：US18525234

申请日：2023-11-30

Applicant: AMYRIS, INC.

Inventor： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC: C12N9/10 ,  A23L2/60 ,  A23L27/30 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017 ,  A23V2002/00

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20240060056A1

公开(公告)日：2024-02-22

申请号：US18267928

申请日：2021-12-16

Applicant: Amyris, Inc.

Inventor： Jessica WALTER ,  Wenzong LI ,  Christopher F. MUGLER ,  Victoria HSIAO

IPC: C12N9/10 ,  C12P19/18 ,  C12N1/16

CPC classification number: C12N9/1051 ,  C12P19/18 ,  C12N1/165 ,  C12Y204/01146 ,  C12R2001/85

Abstract: Provided herein are variant β-1,3-N-acetylglucosaminyltransferase polypeptides capable of producing lacto-n-neotetraose, yeast cells capable of producing one or more human milk oligosaccharides, and methods of making such cells. Also, provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.

公开(公告)号：US20210371892A1

公开(公告)日：2021-12-02

申请号：US17285888

申请日：2019-10-14

Applicant: AMYRIS, INC.

Inventor： Gale A. WICHMANN ,  Wenzong LI ,  Sean LUND ,  Shaina J. JACKSON ,  Chantal V. GARCIA DE GONZALO ,  Hailley WARBINGTON ,  Yi XIONG ,  Svetlana Alekseyevna BORISOVA

IPC: C12P19/56 ,  C12N9/02 ,  C12N9/10 ,  C12N1/18 ,  C12N9/88 ,  C12N9/90

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Stevia rebaudiana kaurenoic acid hydroxylase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20210355458A1

公开(公告)日：2021-11-18

申请号：US17382963

申请日：2021-07-22

Applicant: AMYRIS, INC.

Inventor： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20190185826A1

公开(公告)日：2019-06-20

申请号：US16323756

申请日：2017-08-11

Applicant: AMYRIS, INC.

Inventor： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  GARCIA DE GONZALO, Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC: C12N9/10 ,  C12N15/52 ,  C12P19/56 ,  C12P19/18 ,  A23L2/60 ,  A23L27/30

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.