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公开(公告)号：US20210230234A1
公开(公告)日：2021-07-29
申请号：US17221371
申请日：2021-04-02
IPC分类号： C07K14/395 , C12P19/62 , C12P5/02 , C12P7/64 , C12P21/00 , C12N1/16 , C12N15/52 , C12N15/63 , C12N15/65 , C12P21/02
摘要： The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
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公开(公告)号：US20200270643A1
公开(公告)日：2020-08-27
申请号：US16733191
申请日：2020-01-02
发明人： Penelope R. CHUA , Adam MEADOWS
摘要： The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
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公开(公告)号：US20160177341A1
公开(公告)日：2016-06-23
申请号：US14910240
申请日：2013-08-07
发明人： Penelope R. CHUA , Adam MEADOWS
摘要： The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
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4.发明申请公开(公告)号：US20220307059A1
公开(公告)日：2022-09-29
申请号：US17616843
申请日：2020-06-05
申请人： AMYRIS, INC.
发明人： Penelope R. CHUA , Joshua Adam LERMAN , Thomas Jon SCHERBART , Chandresh THAKKER , Annie Ening TSONG , Hanxiao JIANG
摘要： Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.
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公开(公告)号：US20180186841A1
公开(公告)日：2018-07-05
申请号：US15738555
申请日：2016-06-24
IPC分类号： C07K14/395 , C12P5/02
摘要： The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
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公开(公告)号：US20180171341A1
公开(公告)日：2018-06-21
申请号：US15738918
申请日：2016-06-24
CPC分类号： C07K14/395 , C07K2319/95 , C12N1/16 , C12N15/52 , C12N15/635 , C12N15/65 , C12N2330/51 , C12P5/02 , C12P5/026 , C12P7/64 , C12P19/62 , C12P21/00 , C12P21/02 , C12Y503/03002
摘要： The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
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