公开(公告)号：US20190210027A1

公开(公告)日：2019-07-11

申请号：US15739318

申请日：2016-06-29

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Cody YOUNGBULL ,  Andrew HATCH ,  Tathagata RAY ,  Andrew LARSEN ,  Matthew UNDERHILL

IPC: B01L7/00 ,  G01N1/10 ,  C12Q1/686 ,  B01L3/00 ,  G01N35/10

CPC classification number: B01L7/52 ,  B01L3/50851 ,  C12Q1/686 ,  G01N1/10 ,  G01N35/1095 ,  G01N2035/00465 ,  G01N2035/1032 ,  G01N2035/1034

Abstract: Systems and methods for continuous flow polymerase chain reaction (PCR) are provided. The system comprises an injector, a mixer, a coalescer, a droplet generator, a detector, a digital PCR system, and a controller. The injector takes in a sample, partitions the sample into sample aliquots with the help of an immiscible oil phase, dispenses waste, and sends the sample aliquot to the mixer. The mixer mixes the sample aliquot with a PCR master mix and diluting water, dispenses waste, and sends the sample mixture (separated by an immiscible oil) to the coalescer. The coalescer coalesces the sample mixture with primers dispensed from a cassette, dispenses waste, and sends the reaction mixture (separated by an immiscible oil) to the droplet generator. The droplet generator converts the sample mixture into an emulsion where aqueous droplets of the reaction mixture are maintained inside of an immiscible oil phase and dispenses droplets to the digital PCR system. The digital PCR system amplifies target DNAs in the droplets. The detector detects target DNAs in the droplets. The controller controls the system to run automatically and continuously.

公开(公告)号：US20240342721A1

公开(公告)日：2024-10-17

申请号：US18597605

申请日：2024-03-06

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Cody YOUNGBULL ,  Andrew HATCH ,  Andrew LARSEN ,  Tathagata Ray ,  Matthew UNDERHILL

IPC: B01L3/00 ,  B01F33/302 ,  B01F33/3033 ,  B01L7/00 ,  C12Q1/6848 ,  C12Q1/686

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  C12Q1/6848 ,  B01F33/3021 ,  B01F33/3033 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/16 ,  B01L2300/161 ,  B01L2300/18 ,  B01L2400/0415 ,  C12Q1/686

Abstract: Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.

公开(公告)号：US20230201835A1

公开(公告)日：2023-06-29

申请号：US18111342

申请日：2023-02-17

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Cody YOUNGBULL ,  Andrew HATCH ,  Andrew LARSEN ,  Tathagata RAY ,  Matthew UNDERHILL

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6848

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  C12Q1/6848 ,  C12Q1/686

Abstract: Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.

公开(公告)号：US20230211347A1

公开(公告)日：2023-07-06

申请号：US18114868

申请日：2023-02-27

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Cody YOUNGBULL ,  Andrew HATCH ,  Tathagata RAY ,  Andrew LARSEN ,  Matthew UNDERHILL

IPC: B01L7/00 ,  G01N35/10 ,  B01L3/00 ,  C12Q1/686 ,  G01N1/10

CPC classification number: B01L7/52 ,  G01N35/1095 ,  B01L3/50851 ,  C12Q1/686 ,  G01N1/10 ,  G01N2035/00465

Abstract: Systems and methods for continuous flow polymerase chain reaction (PCR) are provided. The system comprises an injector, a mixer, a coalescer, a droplet generator, a detector, a digital PCR system, and a controller. The injector takes in a sample, partitions the sample into sample aliquots with the help of an immiscible oil phase, dispenses waste, and sends the sample aliquot to the mixer. The mixer mixes the sample aliquot with a PCR master mix and diluting water, dispenses waste, and sends the sample mixture (separated by an immiscible oil) to the coalescer. The coalescer coalesces the sample mixture with primers dispensed from a cassette, dispenses waste, and sends the reaction mixture (separated by an immiscible oil) to the droplet generator. The droplet generator converts the sample mixture into an emulsion where aqueous droplets of the reaction mixture are maintained inside of an immiscible oil phase and dispenses droplets to the digital PCR system. The digital PCR system amplifies target DNAs in the droplets. The detector detects target DNAs in the droplets. The controller controls the system to run automatically and continuously.

公开(公告)号：US20220288591A1

公开(公告)日：2022-09-15

申请号：US17827614

申请日：2022-05-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Cody YOUNGBULL ,  Andrew HATCH ,  Andrew LARSEN ,  Tathagata RAY ,  Matthew UNDERHILL

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6848

Abstract: Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.

公开(公告)号：US20220193681A1

公开(公告)日：2022-06-23

申请号：US17389253

申请日：2021-07-29

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Cody YOUNGBULL ,  Andrew HATCH ,  Tathagata RAY ,  Andrew LARSEN ,  Matthew UNDERHILL

IPC: B01L7/00 ,  G01N35/10 ,  B01L3/00 ,  C12Q1/686 ,  G01N1/10

Abstract: Systems and methods for continuous flow polymerase chain reaction (PCR) are provided. The system comprises an injector, a mixer, a coalescer, a droplet generator, a detector, a digital PCR system, and a controller. The injector takes in a sample, partitions the sample into sample aliquots with the help of an immiscible oil phase, dispenses waste, and sends the sample aliquot to the mixer. The mixer mixes the sample aliquot with a PCR master mix and diluting water, dispenses waste, and sends the sample mixture (separated by an immiscible oil) to the coalescer. The coalescer coalesces the sample mixture with primers dispensed from a cassette, dispenses waste, and sends the reaction mixture (separated by an immiscible oil) to the droplet generator. The droplet generator converts the sample mixture into an emulsion where aqueous droplets of the reaction mixture are maintained inside of an immiscible oil phase and dispenses droplets to the digital PCR system. The digital PCR system amplifies target DNAs in the droplets. The detector detects target DNAs in the droplets. The controller controls the system to run automatically and continuously.