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Patent Agency Ranking
公开(公告)号：US11413616B2
公开(公告)日：2022-08-16
申请号：US16413416
申请日：2019-05-15
Inventor： Cody Youngbull , Andrew Hatch , Andrew Larsen , Tathagata Ray , Matthew Underhill
IPC: B01L3/00 , B01L7/00 , C12Q1/6848 , C12Q1/686 , B01F33/302 , B01F33/3033
Abstract: Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.
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2.发明授权公开(公告)号：US12070754B2
公开(公告)日：2024-08-27
申请号：US18114868
申请日：2023-02-27
Inventor： Cody Youngbull , Andrew Hatch , Tathagata Ray , Andrew Larsen , Matthew Underhill
CPC classification number: B01L7/52 , B01L3/50851 , C12Q1/686 , G01N1/10 , G01N35/1095 , B01F33/3021 , B01L2200/0673 , G01N2035/00465 , G01N2035/1032 , G01N2035/1034
Abstract: Systems and methods for continuous flow polymerase chain reaction (PCR) are provided. The system comprises an injector, a mixer, a coalescer, a droplet generator, a detector, a digital PCR system, and a controller. The injector takes in a sample, partitions the sample into sample aliquots with the help of an immiscible oil phase, dispenses waste, and sends the sample aliquot to the mixer. The mixer mixes the sample aliquot with a PCR master mix and diluting water, dispenses waste, and sends the sample mixture (separated by an immiscible oil) to the coalescer. The coalescer coalesces the sample mixture with primers dispensed from a cassette, dispenses waste, and sends the reaction mixture (separated by an immiscible oil) to the droplet generator. The droplet generator converts the sample mixture into an emulsion where aqueous droplets of the reaction mixture are maintained inside of an immiscible oil phase and dispenses droplets to the digital PCR system. The digital PCR system amplifies target DNAs in the droplets. The detector detects target DNAs in the droplets. The controller controls the system to run automatically and continuously.
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3.发明授权公开(公告)号：US11618030B2
公开(公告)日：2023-04-04
申请号：US17389253
申请日：2021-07-29
Inventor： Cody Youngbull , Andrew Hatch , Tathagata Ray , Andrew Larsen , Matthew Underhill
Abstract: Systems and methods for continuous flow polymerase chain reaction (PCR) are provided. The system comprises an injector, a mixer, a coalescer, a droplet generator, a detector, a digital PCR system, and a controller. The injector takes in a sample, partitions the sample into sample aliquots with the help of an immiscible oil phase, dispenses waste, and sends the sample aliquot to the mixer. The mixer mixes the sample aliquot with a PCR master mix and diluting water, dispenses waste, and sends the sample mixture (separated by an immiscible oil) to the coalescer. The coalescer coalesces the sample mixture with primers dispensed from a cassette, dispenses waste, and sends the reaction mixture (separated by an immiscible oil) to the droplet generator. The droplet generator converts the sample mixture into an emulsion where aqueous droplets of the reaction mixture are maintained inside of an immiscible oil phase and dispenses droplets to the digital PCR system. The digital PCR system amplifies target DNAs in the droplets. The detector detects target DNAs in the droplets. The controller controls the system to run automatically and continuously.
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公开(公告)号：US11607689B2
公开(公告)日：2023-03-21
申请号：US17827614
申请日：2022-05-27
Inventor： Cody Youngbull , Andrew Hatch , Andrew Larsen , Tathagata Ray , Matthew Underhill
IPC: B01L3/00 , B01L7/00 , C12Q1/6848 , C12Q1/686 , B01F33/302 , B01F33/3033
Abstract: Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.
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5.发明授权公开(公告)号：US11123740B2
公开(公告)日：2021-09-21
申请号：US15739318
申请日：2016-06-29
Inventor： Cody Youngbull , Andrew Hatch , Tathagata Ray , Andrew Larsen , Matthew Underhill
Abstract: Systems and methods for continuous flow polymerase chain reaction (PCR) are provided. The system comprises an injector, a mixer, a coalescer, a droplet generator, a detector, a digital PCR system, and a controller. The injector takes in a sample, partitions the sample into sample aliquots with the help of an immiscible oil phase, dispenses waste, and sends the sample aliquot to the mixer. The mixer mixes the sample aliquot with a PCR master mix and diluting water, dispenses waste, and sends the sample mixture (separated by an immiscible oil) to the coalescer. The coalescer coalesces the sample mixture with primers dispensed from a cassette, dispenses waste, and sends the reaction mixture (separated by an immiscible oil) to the droplet generator. The droplet generator converts the sample mixture into an emulsion where aqueous droplets of the reaction mixture are maintained inside of an immiscible oil phase and dispenses droplets to the digital PCR system. The digital PCR system amplifies target DNAs in the droplets. The detector detects target DNAs in the droplets. The controller controls the system to run automatically and continuously.
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