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公开(公告)号：US11156619B2
公开(公告)日：2021-10-26
申请号：US16479373
申请日：2018-01-19
发明人： Liqiang Zhang , Xiangxing Kong , Yanqing Tian , Deirdre Meldrum , Fengyu Su
IPC分类号： G01N21/64 , G01N33/84 , C07D471/16 , C08F220/58 , C12Q1/02 , G01N31/22 , G01N33/50 , G01N21/77
摘要： Fluorescent pH sensors are provided. The fluorescent pH sensor comprises a copolymer for sensing pH and a polymerized form of N-(2-hydroxypropyl)methacrylamide (HPMA) or 2-hydroxyethyl methacrylate (HEMA). The probe for sensing pH has formula (I): wherein R1 and R2 are as defined herein. The fluorescent pH sensors may be used in determining the pH of a sample and detecting extracellular pH in a sample. Methods for preparing the fluorescent pH sensors and the probe for sensing pH are also provided.
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公开(公告)号：US10590155B2
公开(公告)日：2020-03-17
申请号：US16314753
申请日：2017-07-14
发明人： Xiangxing Kong , Yanqing Tian , Fengyu Su , Liqiang Zhang , Deirdre Meldrum
IPC分类号： C07F9/6596 , G01N33/50 , G01N33/58 , G01N33/68
摘要： Mitochondria-targeting potassium sensors and method(s) for making such sensors. The sensor shows a response to potassium and displays a 130-fold dynamic range of fluorescence intensity and high brightness. The sensors response to potassium concentrations was demonstrated to be unaffected by cellular pH value and/or concentrations of other ions. The sensors can be used for monitoring the mitochondrial potassium efflux/influx.
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公开(公告)号：US20230314427A1
公开(公告)日：2023-10-05
申请号：US17617802
申请日：2020-06-15
发明人： Ye Hu , Xiangxing Kong , Tanxi Cai , Qingbo Shu
IPC分类号： G01N33/569 , G01N33/68 , G01N33/543
CPC分类号： G01N33/569 , G01N33/6848 , G01N33/54326 , G01N2458/15 , G01N2446/00 , G01N2333/35
摘要： Provided herein are methods for detecting and identifying disease-specific biomarkers, such as target antigens associated with infection.
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公开(公告)号：US11364502B2
公开(公告)日：2022-06-21
申请号：US15774558
申请日：2016-11-14
发明人： Deirdre Meldrum , Laimonas Kelbauskas , Wacey Teller , Meryl Rodrigues , Hong Wang , Ganquan Song , Yanqing Tian , Fengyu Su , Xiangxing Kong , Liqiang Zhang
摘要： A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.
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5.发明申请公开(公告)号：US20200047182A1
公开(公告)日：2020-02-13
申请号：US15774558
申请日：2016-11-14
发明人： Deirdre Meldrum , Laimonas Kelbauskas , Wacey Teller , Meryl Rodrigues , Hong Wang , Ganquan Song , Yanqing Tian , Fengyu Su , Xiangxing Kong , Liqiang Zhang
IPC分类号： B01L3/00
摘要： A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.
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公开(公告)号：US20190153005A1
公开(公告)日：2019-05-23
申请号：US16314753
申请日：2017-07-14
发明人： Xiangxing Kong , Yanqing Tian , Fengyu Su , Liqiang Zhang , Deirdre Meldrum
IPC分类号： C07F9/6596 , G01N33/50 , G01N33/58 , G01N33/68
CPC分类号： C07F9/6596 , A61K31/69 , C07D498/02 , C07D498/08 , C07D498/18 , G01N33/5079 , G01N33/582 , G01N33/6872
摘要： Mitochondria-targeting potassium sensors and method(s) for making such sensors. The sensor shows a response to potassium and displays a 130-fold dynamic range of fluorescence intensity and high brightness. The sensors response to potassium concentrations was demonstrated to be unaffected by cellular pH value and/or concentrations of other ions. The sensors can be used for monitoring the mitochondrial potassium efflux/influx.
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