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    Methods for identifying inhibitors of chlorophyll synthase
    1.
    发明申请
    Methods for identifying inhibitors of chlorophyll synthase 审中-公开
    鉴定叶绿素合酶抑制剂的方法

    公开(公告)号：US20050130118A1

    公开(公告)日：2005-06-16

    申请号：US10737014

    申请日：2003-12-16

    申请人： Abubakr Aslamkhan ,  Lining Guo ,  Rao Mulpuri ,  Neil Hoffman ,  Susanne Kjemtrup ,  Jocelyn Kearney ,  Cory Christensen ,  Keith Davis ,  Adel Zayed ,  Robert Ascenzi ,  Douglas Boyes

    发明人： Abubakr Aslamkhan ,  Lining Guo ,  Rao Mulpuri ,  Neil Hoffman ,  Susanne Kjemtrup ,  Jocelyn Kearney ,  Cory Christensen ,  Keith Davis ,  Adel Zayed ,  Robert Ascenzi ,  Douglas Boyes

    IPC分类号： C12Q1/00 ,  G01N33/573

    CPC分类号： G01N33/573

    摘要： The present inventors have discovered that antisense suppression of a chlorophyll synthase (CS) gene results in plants exhibiting one or more of chlorosis, reduced growth, and altered development. Thus, the present inventors have discovered that the protein encoded by the CS gene is essential for normal plant growth and development, and is useful as a target for the identification of compounds as antibiotics and herbicides, especially herbicides. The present invention is directed to methods for identifying inhibitors of a CS enzyme by incubating a CS polypeptide with a chlorophyllide and a phopholipid substrate in the presence and absence of a test compound under conditions suitable for the CS enzyme activity, adding a solution to the incubation reactions comprising a water immiscible organic solvent, a water-soluble alcohol, and a water-soluble dye that absorbs in the range of one or both the excitation and emission wavelength ranges of the chlorophyllide substrate, and measuring the fluorescence of the incubation reactions at from about 650 to 750 nm, using from about 425 to 445 nm as excitation wavelength, wherein a decrease in the fluorescence in the presence of the test compound indicates that the compound is a CS inhibitor.

    摘要翻译： 本发明人已经发现，叶绿素合酶（CS）基因的反义抑制导致植物表现出一种或多种褪绿，生长减少和改变发育。 因此，本发明人已经发现，由CS基因编码的蛋白质对于正常的植物生长和发育是必需的，并且可用作鉴定作为抗生素和除草剂，特别是除草剂的化合物的靶标。 本发明涉及通过在适合于CS酶活性的条件下，在存在和不存在测试化合物的情况下，将CS多肽与叶绿素和磷脂底物一起温育来鉴定CS酶抑制剂的方法，向培养基中加入溶液 包括与水不溶混的有机溶剂，水溶性醇和在所述叶绿素底物的激发和发射波长范围中的一个或两个的范围内吸收的水溶性染料的反应，以及测量从 约650至750nm，使用约425至445nm作为激发波长，其中在测试化合物存在下荧光的降低表明该化合物是CS抑制剂。

    HYDROPONIC APPARATUS AND METHODS OF USE
    6.
    发明申请
    HYDROPONIC APPARATUS AND METHODS OF USE 审中-公开
    水力装置及其使用方法

    公开(公告)号：US20120277117A1

    公开(公告)日：2012-11-01

    申请号：US13203516

    申请日：2010-02-26

    申请人： Adel Zayed ,  Michael L. Jones ,  Chris Tierney ,  Matthew Miller

    发明人： Adel Zayed ,  Michael L. Jones ,  Chris Tierney ,  Matthew Miller

    IPC分类号： C40B60/12 ,  C40B30/06

    CPC分类号： A01G7/00 ,  A01G31/02 ,  Y02P60/216

    摘要： Hydroponic apparatus and methods for the high-throughput screening plants are disclosed. In one aspect, a method for the high-throughput screening of plants is disclosed. The method comprises germinating a plurality of plants in a hydroponic apparatus; selecting one or more plants having substantially uniform qualities from the plurality of germinated plants to form a population of plants; growing the population of selected plants in a controlled environment; and screening one or more plants in the population at least once during a growing period to determine the presence or absence of one or more predetermined characteristics

    摘要翻译： 公开了用于高通量筛选装置的水培装置和方法。 一方面，公开了一种用于植物高通量筛选的方法。 该方法包括在水培装置中发芽多个植物; 从所述多个发芽植物中选择具有基本均匀质量的一种或多种植物以形成植物群; 在受控环境中种植选定植物的种群; 以及在生长期间至少一次筛选所述群体中的一种或多种植物以确定是否存在一种或多种预定特征

    Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants
    8.
    发明授权
    Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants 失效
    调节草酸合酶和草酸转化酶表达或活性的方法和组合物

    公开(公告)号：US06465217B1

    公开(公告)日：2002-10-15

    申请号：US09610040

    申请日：2000-07-05

    申请人： Douglas C. Boyes ,  Keith R. Davis ,  Jeffrey P. Woessner ,  Jörn Görlach ,  Carol M. Hamilton ,  Neil E. Hoffman ,  Andreas S. Klöti ,  Adel Zayed ,  Robert A. Ascenzi

    发明人： Douglas C. Boyes ,  Keith R. Davis ,  Jeffrey P. Woessner ,  Jörn Görlach ,  Carol M. Hamilton ,  Neil E. Hoffman ,  Andreas S. Klöti ,  Adel Zayed ,  Robert A. Ascenzi

    IPC分类号： C12N2106

    CPC分类号： G01N33/68 ,  G01N2500/00

    摘要： The present inventors have discovered that chorismate mutase and chorismate synthase are essential for plant growth. Specifically, the inhibition of chorismate mutase or chorismate synthase gene expression in plant seedlings results in severe chlorosis, reduced growth and developmental abnormalities. Thus, in one aspect the invention provides compositions for the modulation of plant growth or development comprising chorismate synthase and chorismate mutase antisense and sense polynucleotides, dsRNA and ribozymes, and related expression cassettes and vectors. The compositions of the invention are particularly useful for the modulation and inhibition of plant growth. The invention further provides plants, plant cells, and seeds containing the polynucleotides of the invention. The inventors have proven that chorismate synthase and chorismate mutase can be used as targets for the identification of herbicides. Thus, the invention provides methods for the identification of chemicals that modulate chorismate synthase and chorismate mutase biochemical reactions. The methods of the invention are useful for the identification of herbicides and for the inhibition of plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of chorismate synthase or chorismate mutase expression or function. Such compounds can be used to promote plant growth and development.

    摘要翻译： 本发明人已经发现，草酸修饰酶和绒毛膜合成酶对于植物生长是必需的。 具体来说，植物幼苗中的chorismate mutase或chorismate synthase gene表达的抑制导致严重的萎黄症，生长发育减退和发育异常。 因此，在一个方面，本发明提供了用于调节植物生长或发育的组合物，其包含绒毛膜合成酶和辅助组氨酸变位酶反义和有义多核苷酸，dsRNA和核酶以及相关的表达盒和载体。 本发明的组合物特别可用于调节和抑制植物生长。 本发明还提供含有本发明的多核苷酸的植物，植物细胞和种子。 本发明人已经证明，可以使用草酸合成酶和氯酸盐变位酶作为鉴定除草剂的靶标。 因此，本发明提供了用于鉴定调节绒毛膜合成酶和氯酸盐变位酶生化反应的化学物质的方法。 本发明的方法可用于鉴定除草剂和用于抑制植物的生长和发育。 此外，本发明的方法可用于鉴定刺激基底膜合成酶或调节酸变位酶表达或功能的表达或功能的化合物。 这些化合物可用于促进植物的生长和发育。

    Methods for the identification of modulators of magnesium chelatase expression or activity in plants
    9.
    发明授权
    Methods for the identification of modulators of magnesium chelatase expression or activity in plants 失效
    鉴定镁螯合酶表达或植物活性调节剂的方法

    公开(公告)号：US06582900B1

    公开(公告)日：2003-06-24

    申请号：US09659310

    申请日：2000-09-12

    申请人： Jeffrey P. Woessner ,  Adel Zayed ,  Jörn Görlach ,  Douglas C. Boyes ,  Keith R. Davis ,  Carol M. Hamilton ,  Neil E. Hoffman ,  Andreas S. Kloti ,  Robert A. Ascenzi

    发明人： Jeffrey P. Woessner ,  Adel Zayed ,  Jörn Görlach ,  Douglas C. Boyes ,  Keith R. Davis ,  Carol M. Hamilton ,  Neil E. Hoffman ,  Andreas S. Kloti ,  Robert A. Ascenzi

    IPC分类号： C12Q100

    CPC分类号： C12Q1/25 ,  A01N61/00 ,  G01N2500/00

    摘要： The present inventors have discovered that Mg-chelatase is essential for the growth of Arabidopsis. Specifically, the inhibition of Mg-chelatase CHL H gene expression in Arabidopsis seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, Arabidopsis Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate Arabidopsis Mg-chelatase expression or activity, comprising: contacting a compound with a Arabidopsis Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of Mg-chelatase expression or function. Such compounds can be used to promote or manipulate plant growth and development.

    摘要翻译： 本发明人已经发现，Mg螯合酶对于拟南芥的生长是必需的。 具体来说，拟南芥幼苗中Mg-螯合酶CHL H基因表达的抑制导致不同程度的褪绿（黄化），显着降低生长和发育异常。 因此，拟南芥Mg螯合酶可用作鉴定除草剂的靶标。 因此，本发明提供了鉴定调节拟南芥Mg-螯合酶表达或活性的化合物的方法，其包括：使化合物与拟南芥Mg-螯合酶或其亚单位接触，并检测其中是否存在结合 所述化合物和所述Mg-螯合酶，或检测Mg螯合酶表达或活性的变化。本发明的方法可用于鉴定可以调节植物生长和发育的除草剂和其它化合物。 此外，本发明的方法可用于鉴定刺激Mg-螯合酶表达或功能的表达或功能的化合物。 这些化合物可用于促进或操纵植物的生长和发育。