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公开(公告)号：US20070123703A1
公开(公告)日：2007-05-31
申请号：US11668174
申请日：2007-01-29
申请人： Achim Krotz , Daniel Capaldi , Hans Gaus , Brett Turney
发明人： Achim Krotz , Daniel Capaldi , Hans Gaus , Brett Turney
IPC分类号： C07H21/04
CPC分类号： C07H21/00
摘要： A process of manufacturing oligonucleotides includes a 5′-deblocking step in which the 5-blocking group is removed with dichloroacetic acid that is essentially free of chloral. The process is useful for making oligonucleotides that are substantially free of chloral adducts.
摘要翻译： 制造寡核苷酸的方法包括5-去封闭步骤，其中5-封闭基团用基本上不含氯醛的二氯乙酸除去。 该方法可用于制备基本上不含氯醛加合物的寡核苷酸。
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公开(公告)号：US20050075490A1
公开(公告)日：2005-04-07
申请号：US10403692
申请日：2003-03-31
申请人： Achim Krotz , Daniel Capaldi , Hans Gaus , Brett Turney
发明人： Achim Krotz , Daniel Capaldi , Hans Gaus , Brett Turney
CPC分类号： C07H21/00
摘要： A process of manufacturing oligonucleotides includes a 5′-deblocking step in which the 5-blocking group is removed with dichloroacetic acid that is essentially free of chloral. The process is useful for making oligonucleotides that are substantially free of chloral adducts.
摘要翻译： 制造寡核苷酸的方法包括5-去封闭步骤，其中5-封闭基团用基本上不含氯醛的二氯乙酸除去。 该方法可用于制备基本上不含氯醛加合物的寡核苷酸。
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公开(公告)号：US07169916B2
公开(公告)日：2007-01-30
申请号：US10403692
申请日：2003-03-31
申请人： Achim Krotz , Daniel Capaldi , Hans Gaus , Brett Turney
发明人： Achim Krotz , Daniel Capaldi , Hans Gaus , Brett Turney
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： A process of manufacturing oligonucleotides includes a 5′-deblocking step in which the 5-blocking group is removed with dichloroacetic acid that is essentially free of chloral. The process is useful for making oligonucleotides that are substantially free of chloral adducts.
摘要翻译： 制造寡核苷酸的方法包括5-去封闭步骤，其中5-封闭基团用基本上不含氯醛的二氯乙酸除去。 该方法可用于制备基本上不含氯醛加合物的寡核苷酸。
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公开(公告)号：US07759480B2
公开(公告)日：2010-07-20
申请号：US11668174
申请日：2007-01-29
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： A process of manufacturing oligonucleotides includes a 5′-deblocking step in which the 5-blocking group is removed with dichloroacetic acid that is essentially free of chloral. The process is useful for making oligonucleotides that are substantially free of chloral adducts.
摘要翻译： 制造寡核苷酸的方法包括5-去封闭步骤，其中5-封闭基团用基本上不含氯醛的二氯乙酸除去。 该方法可用于制备基本上不含氯醛加合物的寡核苷酸。
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公开(公告)号：US20050137390A1
公开(公告)日：2005-06-23
申请号：US11024958
申请日：2004-12-28
申请人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
发明人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
摘要： Synthetic processes are provided wherein oligomeric compounds are prepared having phosphodiester, phosphorothioate, phosphorodithioate, or other covalent linkages. The oligomers have substantially reduced exocyclic adducts deriving from acrylonitrile or related contaminants.
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公开(公告)号：US20070265435A1
公开(公告)日：2007-11-15
申请号：US11696874
申请日：2007-04-05
申请人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
发明人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
IPC分类号： C07H21/00
摘要： Synthetic processes are provided wherein oligomeric compounds are prepared having phosphodiester, phosphorothioate, phosphorodithioate, or other covalent linkages. The oligomers have substantially reduced exocyclic adducts deriving from acrylonitrile or related contaminants.
摘要翻译： 提供合成方法，其中制备具有磷酸二酯，硫代磷酸酯，二硫代磷酸酯或其它共价键的低聚化合物。 低聚物具有显着减少的衍生自丙烯腈或相关污染物的环加成物。
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公开(公告)号：US20050182251A1
公开(公告)日：2005-08-18
申请号：US11025791
申请日：2004-12-28
申请人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
发明人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
摘要： Synthetic processes are provided wherein oligomeric compounds are prepared having phosphodiester, phosphorothioate, phosphorodithioate, or other covalent linkages. The oligomers have substantially reduced exocyclic adducts deriving from acrylonitrile or related contaminants.
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公开(公告)号：US20050137391A1
公开(公告)日：2005-06-23
申请号：US10940360
申请日：2004-09-14
申请人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
发明人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel Capaldi , Achim Krotz , Douglas Cole , Andrei Guzaev
摘要： Synthetic processes are provided wherein oligomeric compounds are prepared having phosphodiester, phosphorothioate, phosphorodithioate, or other covalent linkages. The oligomers have substantially reduced exocyclic adducts deriving from acrylonitrile or related contaminants.
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公开(公告)号：US07276599B2
公开(公告)日：2007-10-02
申请号：US10858917
申请日：2004-06-02
申请人： Max N. Moore , Mark Andrade , Recaldo Carty , Anthony Scozzari , Achim Krotz
发明人： Max N. Moore , Mark Andrade , Recaldo Carty , Anthony Scozzari , Achim Krotz
摘要： The invention provides for methods of manufacturing an oligonucleotide comprising a pentavalent phosphate triester. In particular, the method comprises providing a 5′ blocked-nucleoside, deblocking the 5′ blocked-nucleoside to form a 5′ OH-nucleoside, coupling the 5′ OH-nucleoside with a phosphoramidite to form and oligonucleotide comprising a trivalent phosphite triester; and oxidizing the oligonucleotide comprising a trivalent phosphite triester to the oligonucleotide comprising a pentavalent phosphate triester. In some embodiments, the wash between any of the steps above is with at least one solvent wash comprising a toluene.
摘要翻译： 本发明提供了制备包含五价磷酸三酯的寡核苷酸的方法。 特别地，该方法包括提供5'封闭核苷，解封5'封闭核苷以形成5'OH-核苷，将5'OH-核苷与亚磷酰胺偶联以形成含有三价亚磷酸三酯的寡核苷酸; 并将包含三价亚磷酸酯三酯的寡核苷酸氧化成包含五价磷酸酯三酯的寡核苷酸。 在一些实施方案中，上述任何步骤之间的洗涤是至少一种包含甲苯的溶剂洗涤液。
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公开(公告)号：US07186822B2
公开(公告)日：2007-03-06
申请号：US11025791
申请日：2004-12-28
申请人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel C. Capaldi , Achim Krotz , Douglas L. Cole , Andrei P. Guzaev
发明人： Vasulinga Ravikumar , Muthiah Manoharan , Daniel C. Capaldi , Achim Krotz , Douglas L. Cole , Andrei P. Guzaev
IPC分类号： C07H21/00
摘要： Synthetic processes are provided wherein oligomeric compounds are prepared having phosphodiester, phosphorothioate, phosphorodithioate, or other covalent linkages. The oligomers have substantially reduced exocyclic adducts deriving from acrylonitrile or related contaminants.
摘要翻译： 提供合成方法，其中制备具有磷酸二酯，硫代磷酸酯，二硫代磷酸酯或其它共价键的低聚化合物。 低聚物具有显着减少的衍生自丙烯腈或相关污染物的环加成物。
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