公开(公告)号：US08173359B2

公开(公告)日：2012-05-08

申请号：US11810005

申请日：2007-06-04

申请人： Adrian Ponce ,  Gregory H. Bearman

发明人： Adrian Ponce ,  Gregory H. Bearman

IPC分类号： C12Q1/00 ,  C12Q1/06 ,  G01N33/20

CPC分类号： C12Q1/04 ,  G01N21/6408 ,  G01N33/54306 ,  G01N33/56911 ,  G01N33/84

摘要： A sample of unknown bacterial spores is added to a test strip. The sample of unknown bacterial spores is drawn to a first sample region on the test strip by capillary action. Species-specific antibodies are bound to the sample when the unknown bacterial spores match the species-specific antibodies, otherwise the sample is left unbound. DPA is released from the bacterial spores in the bound sample. The terbium ions are combined with the DPA to form a Tb-DPA complex. The combined terbium ions and DPA are excited to generate a luminescence characteristic of the combined terbium ions and DPA to detect the bacterial spores. A live/dead assay is performed by a release of the DPA for live spores and a release of DPA for all spores. The detection concentrations are compared to determine the fraction of live spores. Lifetime-gated measurements of bacterial spores to eliminate any fluorescence background from organic chromophores comprise labeling the bacterial spore contents with a long-lifetime lumophore and detecting the luminescence after a waiting period. Unattended monitoring of bacterial spores in the air comprises the steps of collecting bacterial spores carried in the air and repeatedly performing the Tb-DPA detection steps above. The invention is also apparatus for performing the various methods disclosed above.

摘要翻译： 将未知细菌孢子的样品加入到测试条中。 未知细菌孢子的样品通过毛细管作用被吸引到测试条上的第一样品区域。 当未知细菌孢子与物种特异性抗体匹配时，特异性抗体与样品结合，否则样品保持未结合。 DPA从结合样品中的细菌孢子中释放出来。 铽离子与DPA组合形成Tb-DPA复合物。 激发组合的铽离子和DPA以产生组合的铽离子和DPA的发光特性以检测细菌孢子。 通过释放活孢子的DPA和释放所有孢子的DPA来进行活/死测定。 比较检测浓度以确定活孢子的分数。 细菌孢子的终生门控测量以消除有机发色团中的任何荧光背景包括用长寿命的荧光团标记细菌孢子含量并在等待期后检测发光。 空气中细菌孢子的无人值守监测包括收集空气中携带的细菌孢子并重复进行上述Tb-DPA检测步骤的步骤。 本发明也是用于执行上述各种方法的装置。

公开(公告)号：US07651862B2

公开(公告)日：2010-01-26

申请号：US10469216

申请日：2002-02-26

申请人： Adrian Ponce ,  Dmitri A. Kossakovski ,  Gregory H. Bearman

发明人： Adrian Ponce ,  Dmitri A. Kossakovski ,  Gregory H. Bearman

IPC分类号： G01N21/17 ,  G01N21/84 ,  G02B6/10

CPC分类号： G01M3/047 ,  G01N21/774 ,  G01N2021/7716 ,  G01N2021/7723 ,  G01N2021/7773 ,  G01N2021/7793 ,  G02B6/02033 ,  G02B6/02104 ,  G02B6/03622

摘要： Optical time domain reflectometry caused by absorption of a volatile or analyte into the fiber optic cladding is used an optical nose. The fiber optics (14) are covered with a gas permeable film (44) which is patterned to leave millimeter wide gas permeable notches (48a-48d). The notches contain a sensing polymer that responds to different gases by expanding or contracting.

摘要翻译： 使用光学鼻子将挥发性或分析物吸收到光纤包层中引起的光学时域反射计。 光纤（14）被透气膜（44）覆盖，该膜被图案化以留下毫米宽的气体可透过的凹口（48a-48d）。 缺口包含通过膨胀或收缩来响应不同气体的感测聚合物。

公开(公告)号：US20080113384A1

公开(公告)日：2008-05-15

申请号：US11810005

申请日：2007-06-04

申请人： Adrian Ponce ,  Gregory H. Bearman

发明人： Adrian Ponce ,  Gregory H. Bearman

IPC分类号： C12M3/00 ,  G01N33/53

CPC分类号： C12Q1/04 ,  G01N21/6408 ,  G01N33/54306 ,  G01N33/56911 ,  G01N33/84

摘要： A sample of unknown bacterial spores is added to a test strip. The sample of unknown bacterial spores is drawn to a first sample region on the test strip by capillary action. Species-specific antibodies are bound to the sample when the unknown bacterial spores match the species-specific antibodies, otherwise the sample is left unbound. DPA is released from the bacterial spores in the bound sample. The terbium ions are combined with the DPA to form a Tb-DPA complex. The combined terbium ions and DPA are excited to generate a luminescence characteristic of the combined terbium ions and DPA to detect the bacterial spores. A live/dead assay is performed by a release of the DPA for live spores and a release of DPA for all spores. The detection concentrations are compared to determine the fraction of live spores. Lifetime-gated measurements of bacterial spores to eliminate any fluorescence background from organic chromophores comprise labeling the bacterial spore contents with a long-lifetime lumophore and detecting the luminescence after a waiting period. Unattended monitoring of bacterial spores in the air comprises the steps of collecting bacterial spores carried in the air and repeatedly performing the Tb-DPA detection steps above. The invention is also apparatus for performing the various methods disclosed above.

摘要翻译： 将未知细菌孢子的样品加入到测试条中。 未知细菌孢子的样品通过毛细管作用被吸引到测试条上的第一样品区域。 当未知细菌孢子与物种特异性抗体匹配时，特异性抗体与样品结合，否则样品保持未结合。 DPA从结合样品中的细菌孢子中释放出来。 铽离子与DPA组合形成Tb-DPA复合物。 激发组合的铽离子和DPA以产生组合的铽离子和DPA的发光特性以检测细菌孢子。 通过释放活孢子的DPA和释放所有孢子的DPA来进行活/死测定。 比较检测浓度以确定活孢子的分数。 细菌孢子的终生门控测量以消除有机发色团中的任何荧光背景包括用长寿命的荧光团标记细菌孢子含量并在等待期后检测发光。 空气中细菌孢子的无人值守监测包括收集空气中携带的细菌孢子并重复进行上述Tb-DPA检测步骤的步骤。 本发明也是用于执行上述各种方法的装置。

公开(公告)号：US09816126B2

公开(公告)日：2017-11-14

申请号：US12553938

申请日：2009-09-03

申请人： Adrian Ponce

发明人： Adrian Ponce

IPC分类号： C12Q1/22 ,  C12Q1/06 ,  G01N21/64 ,  C12Q1/04 ,  C12Q1/00 ,  C12M1/34

CPC分类号： C12Q1/22 ,  C12Q1/06 ,  G01N2021/6439

摘要： A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: a matrix including lanthanide ions is provided on the surface containing the bacterial spores; functionalized aromatic molecules are released from the bacterial spores on the surface; a complex of the lanthanide ion and the aromatic molecule is formed on the surface; the complex of the lanthanide ion and the aromatic molecule is excited to generate a characteristic luminescence of the complex on the surface; and the bacterial spores exhibiting the luminescence of the complex on the surface are detected and quantified.

公开(公告)号：US07611862B2

公开(公告)日：2009-11-03

申请号：US11332788

申请日：2006-01-12

申请人： Adrian Ponce

发明人： Adrian Ponce

IPC分类号： C12Q1/06 ,  C12Q1/04 ,  C12Q1/00 ,  C12M1/34

CPC分类号： C12Q1/06 ,  C12Q1/22 ,  G01N1/2813 ,  G01N21/6428 ,  G01N2001/2826 ,  G01N2001/2833 ,  G01N2021/6439

摘要： A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: bacterial spores are transferred from a place of origin to a test surface, the test surface comprises lanthanide ions. Aromatic molecules are released from the bacterial spores; a complex of the lanthanide ions and aromatic molecules is formed on the test surface, the complex is excited to generate a characteristic luminescence on the test surface; the luminescence on the test surface is detected and quantified.

摘要翻译： 一种用于检测和定量表面上的细菌孢子的方法和装置。 按照以下方法：细菌孢子从原产地转移到试验表面，试验表面包括镧系元素离子。 芳香分子从细菌孢子中释放出来; 在测试表面上形成镧系元素离子和芳族分子的复合物，激发复合物以在测试表面上产生特征发光; 检测和量化测试表面上的发光。

公开(公告)号：US07563615B2

公开(公告)日：2009-07-21

申请号：US11404382

申请日：2006-04-14

申请人： Adrian Ponce

发明人： Adrian Ponce

IPC分类号： C12M1/34

CPC分类号： C12M47/06 ,  G01N1/2214 ,  G01N35/00009

摘要： An apparatus and method for automated monitoring of airborne bacterial spores. The apparatus is provided with an air sampler, a surface for capturing airborne spores, a thermal lysis unit to release DPA from bacterial spores, a source of lanthanide ions, and a spectrometer for excitation and detection of the characteristic fluorescence of the aromatic molecules in bacterial spores complexed with lanthanide ions. In accordance with the method: computer-programmed steps allow for automation of the apparatus for the monitoring of airborne bacterial spores.

摘要翻译： 用于自动监测空气中细菌孢子的装置和方法。 该装置设置有空气取样器，用于捕获空气中孢子的表面，用于从细菌孢子释放DPA的热裂解单元，镧系元素离子源和用于激发和检测细菌中芳族分子的特征荧光的光谱仪 与镧系离子络合的孢子。 根据方法：计算机编程的步骤允许用于监测空气中细菌孢子的装置的自动化。

公开(公告)号：US07608419B2

公开(公告)日：2009-10-27

申请号：US10987202

申请日：2004-11-12

申请人： Adrian Ponce

发明人： Adrian Ponce

IPC分类号： C12Q1/06 ,  C12Q1/04 ,  C12Q1/00 ,  C12M1/34

CPC分类号： C12Q1/22 ,  C12Q1/06 ,  G01N2021/6439

摘要： A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: a matrix including lanthanide ions is provided on the surface containing the bacterial spores; functionalized aromatic molecules are released from the bacterial spores on the surface; a complex of the lanthanide ion and the aromatic molecule is formed on the surface; the complex of the lanthanide ion and the aromatic molecule is excited to generate a characteristic luminescence of the complex on the surface; and the bacterial spores exhibiting the luminescence of the complex on the surface are detected and quantified.

摘要翻译： 一种用于检测和定量表面上的细菌孢子的方法和装置。 根据该方法：在含有细菌孢子的表面上提供包含镧系离子的基质; 官能化芳香族分子从表面的细菌孢子中释放出来; 在表面上形成镧系元素离子和芳香族分子的络合物; 激发镧系离子和芳族分子的络合物以产生表面上络合物的特征发光; 检测并定量表现出表面上复合物发光的细菌孢子。

公开(公告)号：US20100075371A1

公开(公告)日：2010-03-25

申请号：US12553938

申请日：2009-09-03

申请人： Adrian Ponce

发明人： Adrian Ponce

IPC分类号： C12Q1/06 ,  C12M1/00 ,  G06K9/78

CPC分类号： C12Q1/22 ,  C12Q1/06 ,  G01N2021/6439

摘要： A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: a matrix including lanthanide ions is provided on the surface containing the bacterial spores; functionalized aromatic molecules are released from the bacterial spores on the surface; a complex of the lanthanide ion and the aromatic molecule is formed on the surface; the complex of the lanthanide ion and the aromatic molecule is excited to generate a characteristic luminescence of the complex on the surface; and the bacterial spores exhibiting the luminescence of the complex on the surface are detected and quantified.

摘要翻译： 一种用于检测和定量表面上的细菌孢子的方法和装置。 根据该方法：在含有细菌孢子的表面上提供包含镧系离子的基质; 官能化芳香族分子从表面的细菌孢子中释放出来; 在表面上形成镧系元素离子和芳香族分子的络合物; 激发镧系离子和芳族分子的络合物以产生表面上络合物的特征发光; 检测并定量表现出表面上复合物发光的细菌孢子。

公开(公告)号：US20060292664A1

公开(公告)日：2006-12-28

申请号：US11332788

申请日：2006-01-12

申请人： Adrian Ponce

发明人： Adrian Ponce

IPC分类号： C12Q1/04 ,  G06K9/00 ,  C12M1/34

CPC分类号： C12Q1/06 ,  C12Q1/22 ,  G01N1/2813 ,  G01N21/6428 ,  G01N2001/2826 ,  G01N2001/2833 ,  G01N2021/6439

摘要： A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: bacterial spores are transferred from a place of origin to a test surface, the test surface comprises lanthanide ions. Aromatic molecules are released from the bacterial spores; a complex of the lanthanide ions and aromatic molecules is formed on the test surface, the complex is excited to generate a characteristic luminescence on the test surface; the luminescence on the test surface is detected and quantified.

公开(公告)号：US20050136508A1

公开(公告)日：2005-06-23

申请号：US10987202

申请日：2004-11-12

申请人： Adrian Ponce

发明人： Adrian Ponce

IPC分类号： C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/22

CPC分类号： C12Q1/22 ,  C12Q1/06 ,  G01N2021/6439

摘要： A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: a matrix including lanthanide ions is provided on the surface containing the bacterial spores; functionalized aromatic molecules are released from the bacterial spores on the surface; a complex of the lanthanide ion and the aromatic molecule is formed on the surface; the complex of the lanthanide ion and the aromatic molecule is excited to generate a characteristic luminescence of the complex on the surface; and the bacterial spores exhibiting the luminescence of the complex on the surface are detected and quantified.

摘要翻译： 一种用于检测和定量表面上的细菌孢子的方法和装置。 根据该方法：在含有细菌孢子的表面上提供包含镧系离子的基质; 官能化芳香族分子从表面的细菌孢子中释放出来; 在表面上形成镧系元素离子和芳香族分子的络合物; 激发镧系离子和芳族分子的络合物以产生表面上络合物的特征发光; 检测并定量表现出表面上复合物发光的细菌孢子。