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公开(公告)号：US5762997A
公开(公告)日：1998-06-09
申请号：US620161
申请日：1996-03-22
申请人： Akihito Hara , Toshiharu Higuchi , Toru Yakabe , Shigeo Kanda , Eiji Yamamoto
发明人： Akihito Hara , Toshiharu Higuchi , Toru Yakabe , Shigeo Kanda , Eiji Yamamoto
CPC分类号： H01J1/26 , H01J9/04 , H01J2209/012
摘要： A method of forming a coating on an electron emitting cathode, in which (1) a black coating is formed on the inner surface of a cathode sleeve constituting the electron emitting cathode, (2) the cathode sleeve is filled with a suspension as a coating material, and (3) a porous absorbent member is brought into contact with or near an opening portion of the cathode sleeve at the same time or after the cathode sleeve is filled with the suspension, thereby causing the porous absorbent member to absorb an unnecessary portion of the suspension. Thereafter, the cathode sleeve to which the coating material is adhered is heat-treated. As a result, a black coating having a uniform thickness is formed, on the inner surface of the cathode sleeve, as a sintered layer obtained by mixing tungsten having an average particle diameter in a range of 0.5 .mu.m (inclusive) to 2 .mu.m (inclusive) with alumina having an average particle diameter in a range of 0.1 .mu.m (inclusive) to 1 .mu.m (exclusive) at a weight ratio of the tungsten to the alumina in a range of (90:10) to (65:35).
摘要翻译： 一种在电子发射阴极上形成涂层的方法，其中（1）在构成电子发射阴极的阴极套管的内表面上形成黑色涂层，（2）阴极套管填充有作为涂层的悬浮液 材料，和（3）同时或在阴极套筒填充悬浮液之后，使多孔吸收体与阴极套筒的开口部分接触或接近，从而使多孔吸收件吸收不需要的部分 的暂停。 之后，对涂覆材料附着的阴极套进行热处理。 结果，在阴极套筒的内表面上形成具有均匀厚度的黑色涂层，作为通过混合平均粒径在0.5μm（包括）至2μm的范围内的钨而获得的烧结层 （90:10）〜（65：10）的范围内的平均粒径在0.1μm以上至1μm以下的氧化铝（不包括在内），钨与氧化铝的重量比为 35）。
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2.发明授权Cathode assembly incorporating a black layer formed from particles having a specified average particle size 失效包含由具有特定平均粒径的颗粒形成的黑色层的阴极组件
公开(公告)号：US5543682A
公开(公告)日：1996-08-06
申请号：US214280
申请日：1994-03-17
申请人： Akihito Hara , Toshiharu Higuchi , Toru Yakabe , Shigeo Kanda , Eiji Yamamoto
发明人： Akihito Hara , Toshiharu Higuchi , Toru Yakabe , Shigeo Kanda , Eiji Yamamoto
CPC分类号： H01J1/26 , H01J9/04 , H01J2209/012
摘要： A black coating having a uniform thickness is formed, on the inner surface of the cathode sleeve, as a sintered layer obtained by mixing tungsten having an average particle diameter in a range of 0.5 .mu.m (inclusive) to 2 .mu.m (inclusive) with alumina having an average particle diameter in a range of 0.1 .mu.m (inclusive) to 1 .mu.m (exclusive) at a weight ratio of the tungsten to the alumina in a range of (90:10) to (65:35).
摘要翻译： 在阴极套筒的内表面上形成具有均匀厚度的黑色涂层，作为将平均粒径在0.5μm以上至2μm（含）的钨与将 在（90:10）〜（65:35）的范围内，钨与氧化铝的重量比的平均粒径为0.1μm以上且1μm以下的氧化铝。
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3.发明授权
公开(公告)号：US5027029A
公开(公告)日：1991-06-25
申请号：US447904
申请日：1989-12-08
申请人： Toshiharu Higuchi , Sadao Matsumoto , Toru Yakabe , Sakae Kimura
发明人： Toshiharu Higuchi , Sadao Matsumoto , Toru Yakabe , Sakae Kimura
摘要： An indirectly heated type cathode assembly comprises a cathode sleeve having a heater within itself and having an emitter-impregnated type cathode disc fitted at one end, a plurality of straps connected at one end to a lower end portion of the cathode sleeve, and a cylinder holder whose upper end is connected to the other end of each strap, the holder being located outside the cathode sleeve such that it is spaced a predetermined distance apart from the cathode sleeve. A heat reflecting cylinder is located between the cathode sleeve and the holder of the indirectly heated type cathode assembly such that it is coaxial with the cathode sleeve and holder. The heat reflecting cylinder is supported by the holder and each strap extends such that it is not in contact with the heat reflecting cylinder. The strap is made of a Ta-W alloy or a Ta-W-Hf alloy. An electron gun structure comprises the indirectly heated type cathode assembly, a first grid placed in front of the indirectly heated type cathode assembly and an insulation support into which the first grid and the holder of the indirectly heated type cathode assembly are embedded partially and directly through a securing piece, respectively. The cathode disc is hidden, by a heat reflecting cylinder, from view at least that portion of the insulating support which is defined between an embedded spot of the first grid and that of the securing piece.
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4.发明申请Method of synthesizing cdna and method of synthesizing rna chains, and nucleotide-immobilized carrier 有权合成cdna的方法和合成rna链的方法以及核苷酸固定载体公开(公告)号：US20100016566A1
公开(公告)日：2010-01-21
申请号：US11990641
申请日：2006-08-09
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/686 , C12Q2521/107 , C12Q2565/537
摘要： A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.
摘要翻译： 一种使用不溶性载体合成cDNA链的方法，所述不溶性载体在其表面上具有含有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元的聚合物物质和具有衍生自羧酸的基团的第二单元， 与羰基结合的电子吸引取代基，其包括固定多核苷酸用于DNA延伸; 将含有RNA片段，核苷酸单体和逆转录酶或具有聚合酶活性的酶的溶液与不溶性载体的表面接触; 并且使用溶液中包含的RNA片段作为模板，使固定在载体表面上的DNA延伸的多核苷酸伸长，从而形成单链cDNA。
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公开(公告)号：US07968290B2
公开(公告)日：2011-06-28
申请号：US11920559
申请日：2006-05-16
CPC分类号： C12N15/1096 , C12Q1/6834 , C12Q2565/537 , C12Q2527/107
摘要： A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration.
摘要翻译： 一种检测基因的方法，包括将DNA延伸引物固定在具有表面的不溶性载体上，所述不溶性载体具有含有具有磷酸胆碱基团的第一单元的聚合物物质和具有吸电子取代基的羧酸衍生基团的第二单元 与羰基结合; 用引物退火模板DNA片段或RNA片段以进行DNA延伸，以便在引入酶的同时延长DNA引物，从而通过其酶作用使着色试剂着色; 并基于着色程度来判断该基因的DNA片段或RNA片段是否存在。
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公开(公告)号：US20090137406A1
公开(公告)日：2009-05-28
申请号：US12227904
申请日：2007-06-01
申请人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
发明人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
CPC分类号： C12Q1/6837
摘要： The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein.
摘要翻译： 本发明提供一种RNA检测方法，其从含有目标样品的反应体系中检测源自目标样品的靶RNA链，其表面上具有含有第一单元的表面，该第一单元含有来自 构成磷脂的亲水部分的磷酸酯和具有由与羰基结合的吸电子取代基组成的羧酸衍生物基团的第二单元，同时具有至少一个反应空间，所述反应空间具有固定化的 固定在其中的核酸引物。
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7.发明授权Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier 有权合成cDNA的方法和合成RNA链的方法以及核苷酸固定载体公开(公告)号：US08822670B2
公开(公告)日：2014-09-02
申请号：US11990641
申请日：2006-08-09
IPC分类号： C07H21/00
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/686 , C12Q2521/107 , C12Q2565/537
摘要： A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.
摘要翻译： 一种使用不溶性载体合成cDNA链的方法，所述不溶性载体在其表面上具有含有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元的聚合物物质和具有衍生自羧酸的基团的第二单元， 与羰基结合的电子吸引取代基，其包括固定多核苷酸用于DNA延伸; 将含有RNA片段，核苷酸单体和逆转录酶或具有聚合酶活性的酶的溶液与不溶性载体的表面接触; 并且使用溶液中包含的RNA片段作为模板，使固定在载体表面上的DNA延伸的多核苷酸伸长，从而形成单链cDNA。
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公开(公告)号：US08088580B2
公开(公告)日：2012-01-03
申请号：US12227904
申请日：2007-06-01
申请人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
发明人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
CPC分类号： C12Q1/6837
摘要： The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein.
摘要翻译： 本发明提供一种RNA检测方法，其从含有目标样品的反应体系中检测源自目标样品的靶RNA链，其表面上具有含有第一单元的表面，该第一单元含有来自 构成磷脂的亲水部分的磷酸酯和具有由与羰基结合的吸电子取代基组成的羧酸衍生物基团的第二单元，同时具有至少一个反应空间，所述反应空间具有固定化的 固定在其中的核酸引物。
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公开(公告)号：US20090042190A1
公开(公告)日：2009-02-12
申请号：US11920559
申请日：2006-05-16
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6834 , C12Q2565/537 , C12Q2527/107
摘要： A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration.
摘要翻译： 一种检测基因的方法，包括将DNA延伸引物固定在具有表面的不溶性载体上，所述不溶性载体具有含有具有磷酸胆碱基团的第一单元的聚合物物质和具有吸电子取代基的羧酸衍生基团的第二单元 与羰基结合; 用引物退火模板DNA片段或RNA片段以进行DNA延伸，以便在引入酶的同时延长DNA引物，从而通过其酶作用使着色试剂着色; 并基于着色程度来判断该基因的DNA片段或RNA片段是否存在。
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