Method for producing a peptidase
    4.
    发明授权
    Method for producing a peptidase 失效
    肽酶的制备方法

    公开(公告)号:US4228241A

    公开(公告)日:1980-10-14

    申请号:US889877

    申请日:1978-03-24

    摘要: A method is provided for producing a peptidase capable of substantially completely hydrolyzing protein into its constituent amino acids which comprises culturing a strain of filamentous fungus, belonging to one of the species Aspergillus oryzae and Aspergillus soyae and characterized in that said strain is capable of producing said peptidase, in a nutrient culture medium containing at least one substrate selected from the group consisting of a fatty acid having 14, 16, 18 or 20 carbon atoms and a derivative of said fatty acid, and recovering said peptidase from said culture medium. It is particularly effective to use a combination of a sugar ester of a fatty acid and a vegetable oil as substrates in a liquid culture medium.

    摘要翻译: 提供了一种生产能够将蛋白质基本上完全水解成其组成氨基酸的肽酶的方法,其包括培养属于米曲霉和大豆曲霉之一的丝状真菌菌株,其特征在于所述菌株能够产生所述 肽酶,在含有选自具有14,16,18或20个碳原子的脂肪酸和所述脂肪酸的衍生物的至少一种底物的营养培养基中,并从所述培养基中回收所述肽酶。 在液体培养基中使用脂肪酸的糖酯和植物油的组合物作为底物是特别有效的。