公开(公告)号：US5440018A

公开(公告)日：1995-08-08

申请号：US36387

申请日：1993-03-24

申请人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Fuluhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama

发明人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Fuluhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama

IPC分类号： A61K38/00 ,  C07K1/14 ,  C07K14/765 ,  C12N15/14 ,  A61K38/38 ,  C07K1/16 ,  C07K1/36

CPC分类号： C07K14/765 ,  A61K38/00

摘要： Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

摘要翻译： 通过基因操作技术获得的人血清白蛋白可以通过特定步骤的组合来纯化，其中将得自人血清白蛋白的宿主的培养上清液进行超滤，热处理，酸处理和另一次超滤，然后用 阳离子交换剂，疏水性色谱载体和阴离子交换剂，并通过盐析，从而获得纯的形式的人血清白蛋白，其基本上不含有配制成药物制剂的蛋白质和多糖污染物。 该方法使得可以有效地纯化重组人血清白蛋白并提供基本上纯的人血清白蛋白，其不含生产者宿主相关物质和其它污染物，并且没有着色。

公开(公告)号：US20050243236A1

公开(公告)日：2005-11-03

申请号：US11115283

申请日：2005-04-27

申请人： Akinori Sumi

发明人： Akinori Sumi

IPC分类号： G02F1/1335 ,  G02F1/1343 ,  G02F1/136 ,  G02F1/1368

CPC分类号： G02F1/133512 ,  G02F1/134363

摘要： A liquid crystal device includes a first substrate, a second substrate arranged oppositely to the first substrate, and a liquid crystal layer sandwiched between the first and second substrates. Preferably, the first substrate includes a gate wiring, a gate terminal part formed at an end of the first substrate and provided with a gate terminal to which a signal to the gate wiring is inputted, a source wiring intersecting with the gate wiring through an insulating film, a switching element connected to the source wiring, a pixel electrode connected to the source wiring through the switching element, and a common electrode arranged oppositely to the pixel electrode. Preferably, the second substrate includes a light shielding film formed on the side of the first substrate and arranged at a position corresponding to the gate wiring, and a cutting where the light shielding film is cut in a pixel near the gate terminal part.

摘要翻译： 液晶装置包括第一基板，与第一基板相对布置的第二基板和夹在第一和第二基板之间的液晶层。 优选地，第一基板包括栅极布线，栅极端子部分，形成在第一基板的端部处并且设置有栅极端子，栅极端子输入到栅极布线的信号;源极布线，其通过绝缘体与栅极布线相交 连接到源极布线的开关元件，通过开关元件连接到源极布线的像素电极，以及与像素电极相对布置的公共电极。 优选地，第二基板包括形成在第一基板侧并且布置在对应于栅极布线的位置处的遮光膜，以及在栅极端子部分附近的像素中切割遮光膜的切割。

公开(公告)号：US5369020A

公开(公告)日：1994-11-29

申请号：US031823

申请日：1993-03-16

申请人： Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Takao Ohmura ,  Kazumasa Yokoyama

发明人： Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Takao Ohmura ,  Kazumasa Yokoyama

IPC分类号： A61K38/16 ,  A61P7/00 ,  B01D15/00 ,  C07K14/765 ,  C12N1/15 ,  C12N1/19 ,  C12N15/09 ,  C12N15/14 ,  C12P21/00 ,  C12P21/02 ,  C12R1/84 ,  C12R1/865 ,  C12P1/02

CPC分类号： C12P21/02 ,  C07K14/765

摘要： A method for suppressing coloring of human serum albumin expressed by genetic engineering, which comprises culture and/or purification in the presence of an amine compound selected from the group consisting of alkylamines, diamines, guanidines, benzamidines, basic amino acids, and aminophenylacetic acids. According to the present invention, coloring of HSA expressed by genetic engineering can be suppressed to from one-half to one-tenth of that without treatment for coloring suppression. In addition, HSA can be recovered in high yields, and the treatment of the invention does not affect the inherent properties of HSA.

摘要翻译： 一种用于抑制由遗传工程表达的人血清白蛋白着色的方法，其包括在选自烷基胺，二胺，胍胺，苯甲脒，碱性氨基酸和氨基苯基乙酸的胺化合物存在下培养和/或纯化。 根据本发明，通过遗传工程表达的HSA的着色可以抑制在不进行着色抑制处理的情况下的一半到十分之一。 另外，HSA可以以高收率回收，本发明的处理不影响HSA的固有特性。

公开(公告)号：US20080117370A1

公开(公告)日：2008-05-22

申请号：US12015267

申请日：2008-01-16

申请人： Akinori Sumi ,  Yasuhiro Morii

发明人： Akinori Sumi ,  Yasuhiro Morii

IPC分类号： G02F1/1335

CPC分类号： G02F1/13394 ,  G02F1/134363 ,  G02F1/136209 ,  G02F1/136286 ,  G02F2001/136222

摘要： A liquid crystal display device has the first substrate and the second substrate. The first substrate includes a gate line, a source line crossing the gate line, a switching element connected to the source line, a liquid crystal drive electrode connected to the switching element and having a plurality of electrodes substantially parallel to each other, and a common electrode consisting of a plurality of comb-shaped electrodes arranged substantially parallel to and alternately with the liquid crystal drive electrode. The second substrate includes color filters arranged in an array arrangement and a black matrix provided between the color filters. A spacer between the substrates contacts the first substrate in the area where the gate line, the liquid crystal drive electrode, and the common electrode are arranged in the vicinity of each other.

摘要翻译： 液晶显示装置具有第一基板和第二基板。 第一基板包括栅极线，与栅极线交叉的源极线，连接到源极线的开关元件，连接到开关元件并具有彼此大致平行的多个电极的液晶驱动电极，以及共同的 电极由与液晶驱动电极大致平行且交替配置的多个梳状电极构成。 第二基板包括以阵列布置布置的滤色器和设置在滤色器之间的黑矩阵。 衬底之间的间隔件在栅极线，液晶驱动电极和公共电极彼此附近的区域中接触第一衬底。

公开(公告)号：US07345731B2

公开(公告)日：2008-03-18

申请号：US10438971

申请日：2003-05-16

申请人： Akinori Sumi ,  Yasuhiro Morii

发明人： Akinori Sumi ,  Yasuhiro Morii

IPC分类号： G02F1/1339

CPC分类号： G02F1/13394 ,  G02F1/134363 ,  G02F1/136209 ,  G02F1/136286 ,  G02F2001/136222

摘要： A liquid crystal display device has the first substrate and the second substrate. The first substrate includes a gate line, a source line crossing the gate line, a switching element connected to the source line, a liquid crystal drive electrode connected to the switching element and having a plurality of electrodes substantially parallel to each other, and a common electrode consisting of a plurality of comb-shaped electrodes arranged substantially parallel to and alternately with the liquid crystal drive electrode. The second substrate includes color filters arranged in an array arrangement and a black matrix provided between the color filters. A spacer between the substrates contacts the first substrate in the area where the gate line, the liquid crystal drive electrode, and the common electrode are arranged in the vicinity of each other.

摘要翻译： 液晶显示装置具有第一基板和第二基板。 第一基板包括栅极线，与栅极线交叉的源极线，连接到源极线的开关元件，连接到开关元件并具有彼此大致平行的多个电极的液晶驱动电极，以及共同的 电极由与液晶驱动电极大致平行且交替配置的多个梳状电极构成。 第二基板包括以阵列布置布置的滤色器和设置在滤色器之间的黑矩阵。 衬底之间的间隔件在栅极线，液晶驱动电极和公共电极彼此附近的区域中接触第一衬底。

公开(公告)号：US5521287A

公开(公告)日：1996-05-28

申请号：US202130

申请日：1994-02-25

申请人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama ,  Nagatoshi Fujiwara

发明人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama ,  Nagatoshi Fujiwara

IPC分类号： A61K38/00 ,  C07K14/765 ,  A61K38/38 ,  C07K1/16 ,  C07K1/36

CPC分类号： C07K14/765 ,  A61K2039/545 ,  A61K38/00

摘要： Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. The thus obtained human serum albumin can further be purified by treating recombinant human serum albumin with a hydrophobic chromatography carrier at pH of 2 to 5 and a salt concentration of 0.4 to 1 and exposing the carrier to a pH of 6 to 8 and a salt concentration of 0.01 to 0.3 M, or treating the culture supernatant with boric acid or a salt thereof at pH 8 to 11 for 1 to 10 hours and recovering the supernatant. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

公开(公告)号：US5294699A

公开(公告)日：1994-03-15

申请号：US719443

申请日：1991-06-24

申请人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Fuluhata ,  Kaoru Kobayashi ,  Shinobu Kuwae ,  Hirotoshi Fukutsuka ,  Tomoshi Ohya ,  Hiroshi Morise

发明人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Fuluhata ,  Kaoru Kobayashi ,  Shinobu Kuwae ,  Hirotoshi Fukutsuka ,  Tomoshi Ohya ,  Hiroshi Morise

IPC分类号： A61K38/16 ,  A61K38/00 ,  B01D15/00 ,  B01D15/04 ,  C07K1/14 ,  C07K1/20 ,  C07K14/435 ,  C07K14/76 ,  C07K14/765 ,  C12N15/09 ,  C12N15/14 ,  C12N15/81 ,  C12P21/02 ,  C12R1/865 ,  C07K3/28 ,  A61K45/02

CPC分类号： C07K14/765 ,  C12N15/81 ,  A61K38/00 ,  C07K2319/02

摘要： A method of inhibiting the coloration of human serum albumin expressed by using the gene manipulation technology which method comprises separating coloring contaminants from said human serum albumin before said coloring contaminants bind to the human serum albumin.

摘要翻译： 通过使用基因操作技术抑制人血清白蛋白的着色的方法，该方法包括在所述着色污染物与人血清白蛋白结合之前从所述人血清白蛋白中分离着色污染物。

公开(公告)号：US07428034B2

公开(公告)日：2008-09-23

申请号：US12015267

申请日：2008-01-16

申请人： Akinori Sumi ,  Yasuhiro Morii

发明人： Akinori Sumi ,  Yasuhiro Morii

IPC分类号： G02F1/1339

CPC分类号： G02F1/13394 ,  G02F1/134363 ,  G02F1/136209 ,  G02F1/136286 ,  G02F2001/136222

摘要： A liquid crystal display device has the first substrate and the second substrate. The first substrate includes a gate line, a source line crossing the gate line, a switching element connected to the source line, a liquid crystal drive electrode connected to the switching element and having a plurality of electrodes substantially parallel to each other, and a common electrode consisting of a plurality of comb-shaped electrodes arranged substantially parallel to and alternately with the liquid crystal drive electrode. The second substrate includes color filters arranged in an array arrangement and a black matrix provided between the color filters. A spacer between the substrates contacts the first substrate in the area where the gate line, the liquid crystal drive electrode, and the common electrode are arranged in the vicinity of each other.

公开(公告)号：US20050046779A1

公开(公告)日：2005-03-03

申请号：US10438971

申请日：2003-05-16

申请人： Akinori Sumi ,  Yasuhiro Morii

发明人： Akinori Sumi ,  Yasuhiro Morii

IPC分类号： G02F1/1339 ,  G02F1/1343 ,  G02F1/1362

CPC分类号： G02F1/13394 ,  G02F1/134363 ,  G02F1/136209 ,  G02F1/136286 ,  G02F2001/136222

摘要： A liquid crystal display device has the first substrate and the second substrate. The first substrate includes a gate line, a source line crossing the gate line, a switching element connected to the source line, a liquid crystal drive electrode connected to the switching element and having a plurality of electrodes substantially parallel to each other, and a common electrode consisting of a plurality of comb-shaped electrodes arranged substantially parallel to and alternately with the liquid crystal drive electrode. The second substrate includes color filters arranged in an array arrangement and a black matrix provided between the color filters. A spacer between the substrates contacts the first substrate in the area where the gate line, the liquid crystal drive electrode, and the common electrode are arranged in the vicinity of each other.

摘要翻译： 液晶显示装置具有第一基板和第二基板。 第一基板包括栅极线，与栅极线交叉的源极线，连接到源极线的开关元件，连接到开关元件并具有彼此大致平行的多个电极的液晶驱动电极，以及共同的 电极由与液晶驱动电极大致平行且交替配置的多个梳状电极构成。 第二基板包括以阵列布置布置的滤色器和设置在滤色器之间的黑矩阵。 衬底之间的间隔件在栅极线，液晶驱动电极和公共电极彼此附近的区域中接触第一衬底。

公开(公告)号：US06480256B2

公开(公告)日：2002-11-12

申请号：US09729673

申请日：2000-12-05

申请人： Akinori Sumi ,  Ichiro Takasaki ,  Miyuki Hirosue

发明人： Akinori Sumi ,  Ichiro Takasaki ,  Miyuki Hirosue

IPC分类号： G02F11333

CPC分类号： G02F1/136204 ,  G02F1/1345

摘要： In a process for chamfering substrates constituting a liquid crystal display device, it is made possible to prevent a static electricity from generating in a chamfering amount mark used in order to perform a highly accurate chamfering, and improve a chamfering accuracy. There is provided a structure in which a chamfering amount mark 7 formed in an end portion of a 1st substrate is formed so as to be electrically connected to a short wiring 10, and a connection between the chamfering amount mark 7 and extension wirings 9 which are another conductive wirings is performed, thereby discharging the static electricity to another conductive pattern. Incidentally, there is adopted a structure in which plural mark patterns 8b constituting the chamfering amount mark 7 are intended to be electrically connected by a connecting wiring 8a and, additionally, the connecting wiring 8a is extended to a short wiring 10 side.

摘要翻译： 在构成液晶显示装置的基板的倒角加工方法中，为了进行高精度的倒角，能够防止以所使用的倒角量标记产生静电，提高倒角精度。 提供一种结构，其中形成在第一基板的端部中的倒角量标记7形成为电连接到短布线10，并且倒角量标记7和延伸布线9之间的连接是 执行另一导电布线，从而将静电放电到另一导电图案。 顺便提及，采用构成倒角量标记7的多个标记图案8b通过连接布线8a电连接的结构，另外，连接布线8a延伸到短布线10侧。