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    METHOD FOR THE EXTRACTION OF ONE OR SEVERAL PROTEINS IN MILK
    1.
    发明申请
    METHOD FOR THE EXTRACTION OF ONE OR SEVERAL PROTEINS IN MILK 审中-公开
    在奶中提取一种或几种蛋白质的方法

    公开(公告)号：US20100047428A1

    公开(公告)日：2010-02-25

    申请号：US12300495

    申请日：2007-05-31

    申请人： Alain Lejars ,  Michel Nogre ,  Michael Tellier

    发明人： Alain Lejars ,  Michel Nogre ,  Michael Tellier

    IPC分类号： A23C7/04 ,  C07K14/745 ,  A23C9/20

    CPC分类号： C07K14/745 ,  C07K1/303 ,  C07K1/36 ,  C12N9/644 ,  C12Y304/21022

    摘要： The invention is related to a process for extracting at least one protein present in milk, said protein exhibiting an affinity for the complexed or non-complexed calcium ions of said milk, comprising the following steps consisting of: a) releasing the protein by precipitation of calcium compounds obtained by contacting the milk with a soluble salt, the anion of which is selected for its capability to form said insoluble calcium compounds in such a medium, in order to obtain in this way a protein-enriched liquid phase, b) separating the protein-enriched liquid phase from the precipitate of calcium compounds, said liquid phase being, moreover, separated in a lipidic phase and in a non-lipidic aqueous phase comprising the protein, and c) recovering the non-lipidic aqueous phase comprising the protein.

    摘要翻译： 本发明涉及一种用于提取乳中存在的至少一种蛋白质的方法，所述蛋白质对所述乳的络合或非络合钙离子表现出亲和力，包括以下步骤：a）通过沉淀来释放蛋白质 通过使牛奶与可溶性盐接触获得的钙化合物，其可选择的阴离子是为了在这种介质中形成所述不溶性钙化合物的能力，以便以这种方式获得富含蛋白质的液相，b）将 此外，在脂质相和包含蛋白质的非脂质水相中分离所述液相，和c）回收包含蛋白质的非脂质水相。

    METHOD FOR EXTRACTING A PROTEIN FROM MILK
    2.
    发明申请
    METHOD FOR EXTRACTING A PROTEIN FROM MILK 有权
    从乳汁中提取蛋白质的方法

    公开(公告)号：US20090281283A1

    公开(公告)日：2009-11-12

    申请号：US12520826

    申请日：2008-01-02

    申请人： Alain Lejars ,  Michel Nogre ,  Monique Ollivier

    发明人： Alain Lejars ,  Michel Nogre ,  Monique Ollivier

    IPC分类号： A23J1/00 ,  A23C9/148 ,  C07K14/745

    CPC分类号： C12N9/6437 ,  A23J1/20 ,  A61D19/04 ,  B01D15/327 ,  B01D15/36 ,  B01D15/3847 ,  C07K1/18 ,  C07K14/745

    摘要： The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing said protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and a ionic characteristic in pH conditions enabling the protein to be retained on said substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluded fraction by removing the milk proteins from said eluded fraction; and e) recovering said protein.

    摘要翻译： 本发明涉及一种从牛奶中提取蛋白质的方法，其具有至少一种疏水口袋和对牛奶的天然pH值的负电荷，其包括以下步骤：a）牛奶的脱脂和脱脂; b）将含有所述蛋白质的脱脂和脱脂部分在含有在pH条件下具有疏水特性和离子特征的配体的色谱底物上通过，使蛋白质保留在所述底物上，pH高于4.6; c）蛋白质的洗脱; d）通过从所述脱落级分中除去乳蛋白来纯化所述脱落级分; 和e）回收所述蛋白质。

    METHOD FOR EXTRACTING A PROTEIN FROM MILK
    3.
    发明申请
    METHOD FOR EXTRACTING A PROTEIN FROM MILK 审中-公开
    从乳汁中提取蛋白质的方法

    公开(公告)号：US20130295646A1

    公开(公告)日：2013-11-07

    申请号：US13910517

    申请日：2013-06-05

    申请人： Alain LEJARS ,  Michel Nogre ,  Monique Ollivier

    发明人： Alain LEJARS ,  Michel Nogre ,  Monique Ollivier

    IPC分类号： C12N9/64 ,  C07K14/745 ,  A61D19/04 ,  C07K1/18

    CPC分类号： C12N9/6437 ,  A23J1/20 ,  A61D19/04 ,  B01D15/327 ,  B01D15/36 ,  B01D15/3847 ,  C07K1/18 ,  C07K14/745

    摘要： The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing the protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and an ionic characteristic in pH conditions enabling the protein to be retained on the substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluted fraction by removing the milk proteins from the eluted fraction; and e) recovering the protein.

    摘要翻译： 本发明涉及一种从牛奶中提取蛋白质的方法，其具有至少一种疏水口袋和对牛奶的天然pH值的负电荷，其包括以下步骤：a）牛奶的脱脂和脱脂; b）将含有蛋白质的脱脂和脱脂级分在含有在pH条件下具有疏水特性和离子特征的配体的色谱底物上通过，使蛋白质保留在底物上，pH高于4.6; c）蛋白质的洗脱; d）通过从洗脱的级分中除去乳蛋白来纯化洗脱级分; 和e）回收蛋白质。

    Method for extracting a protein from milk
    4.
    发明授权
    Method for extracting a protein from milk 有权
    从牛奶中提取蛋白质的方法

    公开(公告)号：US08492524B2

    公开(公告)日：2013-07-23

    申请号：US12520826

    申请日：2008-01-02

    申请人： Alain Lejars ,  Michel Nogre ,  Monique Ollivier

    发明人： Alain Lejars ,  Michel Nogre ,  Monique Ollivier

    IPC分类号： C07K14/745 ,  A23C9/148 ,  A23J1/20

    CPC分类号： C12N9/6437 ,  A23J1/20 ,  A61D19/04 ,  B01D15/327 ,  B01D15/36 ,  B01D15/3847 ,  C07K1/18 ,  C07K14/745

    摘要： The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing the protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and an ionic characteristic in pH conditions enabling the protein to be retained on the substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluted fraction by removing the milk proteins from the eluted fraction; and e) recovering the protein.

    摘要翻译： 本发明涉及一种从牛奶中提取蛋白质的方法，其具有至少一种疏水口袋和对牛奶的天然pH值的负电荷，其包括以下步骤：a）牛奶的脱脂和脱脂; b）将含有蛋白质的脱脂和脱脂级分在含有在pH条件下具有疏水特性和离子特征的配体的色谱底物上通过，使蛋白质保留在底物上，pH高于4.6; c）蛋白质的洗脱; d）通过从洗脱的级分中除去乳蛋白来纯化洗脱级分; 和e）回收蛋白质。